Hydroxylation of multi-walled carbon nanotubes: Enhanced biocompatibility through reduction of oxidative stress initiated cell membrane damage, cell cycle arrestment and extrinsic apoptotic pathway

2016 ◽  
Vol 47 ◽  
pp. 124-130 ◽  
Author(s):  
Zhenbao Liu ◽  
Yanfei Liu ◽  
Dongming Peng
Keyword(s):  
Oxidative Stress ◽  
Cell Cycle ◽  
Carbon Nanotubes ◽  
Cell Membrane ◽  
Membrane Damage ◽  
Apoptotic Pathway ◽  
Damage Cell ◽  
Cell Membrane Damage ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

scholarly journals Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice

2020 ◽  
Vol 7 ◽  
Author(s):  
Omid Farshad ◽  
Reza Heidari ◽  
Mohammad Javad Zamiri ◽  
Socorro Retana-Márquez ◽  
Meghdad Khalili ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Carbon Nanotubes ◽  
Sperm Count ◽  
Weight Coefficient ◽  
Reproductive Organs ◽  
Significant Decrement ◽  
Total Antioxidant ◽  
Multi Walled Carbon Nanotubes ◽  
Carbon Based Nanomaterials ◽  
Walled Carbon Nanotubes

Carbon-based nanomaterials possess a remarkably high potential for biomedical applications due to their physical properties; however, their detrimental effects on reproduction are also concerned. Several reports indicate the toxicity of carbon nanotubes (CNT); nevertheless, their impact on intracellular organelles in the male reproductive organs has not been fully elucidated. Herein, we report on the reprotoxicity of single-walled (SWCNT) and multi-walled carbon nanotubes (MWCN) on several intracellular events and histological criteria in pubertal male BALB/c mice orally treated with 0, 10, and 50 mg/kg/day doses for 5 weeks. Biomarkers of oxidative stress and mitochondrial functionality, histopathological alterations, and epididymal sperm characteristics were determined. Oral administration of CNTs at 10 and 50 mg/kg evoked a significant decrement in weight coefficient, sperm viability and motility, hypo-osmotic swelling (HOS) test, sperm count, mitochondrial dehydrogenase activity, ATP content, total antioxidant capacity, and GSH/GSSH ratio in the testis and epididymal spermatozoa. On the other hand, percent abnormal sperm, testicular and sperm TBARS contents, protein carbonylation, ROS formation, oxidized glutathione level, and sperm mitochondrial depolarization were considerably increased. Significant histopathological and stereological alterations in the testis occurred in the groups challenged with CNTs. The current findings indicated that oxidative stress and mitochondrial impairment might substantially impact CNTs-induced reproductive system injury and sperm toxicity. The results can also be used to establish environmental standards for CNT consumption by mammals, produce new chemicals for controlling the rodent populations, and develop therapeutic approaches against CNTs-associated reproductive anomalies in the males exposed daily to these nanoparticles.

scholarly journals Short term exposure to multi-walled carbon nanotubes induce oxidative stress and DNA damage in Xenopus laevis tadpoles

2014 ◽  
Vol 107 ◽  
pp. 22-29 ◽  
Author(s):  
Rayenne Saria ◽  
Florence Mouchet ◽  
Annie Perrault ◽  
Emmanuel Flahaut ◽  
Christophe Laplanche ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Carbon Nanotubes ◽  
Dna Damage ◽  
Xenopus Laevis ◽  
Short Term ◽  
Short Term Exposure ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

Mechanisms of Multi-walled Carbon Nanotubes–Induced Oxidative Stress and Genotoxicity in Mouse Fibroblast Cells

2015 ◽  
Vol 34 (3) ◽  
pp. 258-265 ◽  
Author(s):  
Saud Alarifi ◽  
Daoud Ali
Keyword(s):  
Oxidative Stress ◽  
Carbon Nanotubes ◽  
Underlying Mechanism ◽  
Neutral Red ◽  
Mouse Fibroblast ◽  
Fibroblast Cells ◽  
L929 Cells ◽  
Multi Walled Carbon Nanotubes ◽  
Dna Strand ◽  
Walled Carbon Nanotubes

The extensive production and wide application of carbon nanotubes have made investigations of its toxic potentials necessary. In the present study, we explored the underlying mechanism through which multi-walled carbon nanotubes (MWCNTs) induce toxicity in mouse fibroblast cells (L929). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and neutral red uptake viability assays were used to examine mechanisms of cytotoxicity. Dose and time-dependent cytotoxicity was observed in L929 cells. The MWCNTs significantly increased the generation of reactive oxygen species, lipid peroxidation, superoxide dismutase, and decreased glutathione. It was observed that the MWCNTs induced caspase 3 activity. The highest DNA strand breakage was detected by comet assay at 300 µg/mL of MWCNTs. Thus, the data indicate that MWCNTs induced cytotoxicity and apoptosis in L929 cells via oxidative stress.

Protective effect of diosmetin on in vitro cell membrane damage and oxidative stress in cultured rat hepatocytes

Toxicology ◽  
1992 ◽  
Vol 73 (2) ◽  
pp. 179-189 ◽  
Author(s):  
Pia Villa ◽  
Dario Cova ◽  
Laura De Francesco ◽  
Amalia Guaitani ◽  
Giuseppina Palladini ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Membrane ◽  
Protective Effect ◽  
Membrane Damage ◽  
Rat Hepatocytes ◽  
Cell Membrane Damage ◽  
Cultured Rat Hepatocytes ◽  
And Oxidative Stress

scholarly journals Internalization, cytotoxicity, oxidative stress and inflammation of multi-walled carbon nanotubes in human endothelial cells: influence of pre-incubation with bovine serum albumin

RSC Advances ◽  
2018 ◽  
Vol 8 (17) ◽  
pp. 9253-9260 ◽  
Author(s):  
Jimin Long ◽  
Xianqiang Li ◽  
Yang Kang ◽  
Yanhuai Ding ◽  
Zhipeng Gu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Carbon Nanotubes ◽  
Endothelial Cells ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Human Blood ◽  
Bovine Serum ◽  
Human Endothelial Cells ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

When entering circulation, multi-walled carbon nanotubes (MWCNTs) will inevitably adsorb proteins, which can consequently influence their toxicity to cells lining human blood vessels.

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