Diphlorethohydroxycarmalol attenuated cell damage against UVB radiation via enhancing antioxidant effects and absorbing UVB ray in human HaCaT keratinocytes

2013 ◽  
Vol 36 (2) ◽  
pp. 680-688 ◽  
Author(s):  
Mei Jing Piao ◽  
Kyoung Ah Kang ◽  
Ki Cheon Kim ◽  
Sungwook Chae ◽  
Gi Ok Kim ◽  
...  
Keyword(s):  
Cell Damage ◽  
Uvb Radiation ◽  
Hacat Keratinocytes ◽  
Antioxidant Effects

scholarly journals Empetrum nigrumvar.japonicumExtract Suppresses Ultraviolet B-Induced Cell Damage via Absorption of Radiation and Inhibition of Oxidative Stress

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Ki Cheon Kim ◽  
Daeshin Kim ◽  
Sang Cheol Kim ◽  
Eunsun Jung ◽  
Deokhoon Park ◽  
...  
Keyword(s):  
Apoptotic Cell ◽  
Cell Damage ◽  
Human Keratinocytes ◽  
Ultraviolet B ◽  
Cellular Damage ◽  
Peroxidation Of Lipids ◽  
Uvb Radiation ◽  
Hacat Keratinocytes ◽  
Dna Strands ◽  
Cellular Components

This study focused on the protective actions ofEmpetrum nigrumagainst ultraviolet B (UVB) radiation in human HaCaT keratinocytes. An ethyl acetate extract ofE. nigrum(ENE) increased cell viability decreased by exposure to UVB rays. ENE also absorbed UVB radiation and scavenged UVB-induced intracellular reactive oxygen species (ROS) in HaCaT keratinocytes. In addition, ENE shielded HaCaT keratinocytes from damage to cellular components (e.g., peroxidation of lipids, modification of proteins, and breakage of DNA strands) following UVB irradiation. Furthermore, ENE protected against UVB-induced apoptotic cell death, as determined by a reduction in the numbers of apoptotic bodies and sub-G1hypodiploid cells, as well as by the recovery of mitochondrial membrane potential. The results of the current study therefore suggest that ENE safeguards human keratinocytes against UVB-induced cellular damage via the absorption of UVB ray and scavenging of UVB-generated ROS.

scholarly journals Rosmarinic Acid Attenuates Cell Damage against UVB Radiation-Induced Oxidative Stress via Enhancing Antioxidant Effects in Human HaCaT Cells

2016 ◽  
Vol 24 (1) ◽  
pp. 75-84 ◽  
Author(s):  
Pattage Madushan Dilhara Jayatissa Fernando ◽  
Mei Jing Piao ◽  
Kyoung Ah Kang ◽  
Yea Seong Ryu ◽  
Susara Ruwan Kumara Madduma Hewage ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rosmarinic Acid ◽  
Cell Damage ◽  
Hacat Cells ◽  
Uvb Radiation ◽  
Radiation Induced ◽  
Antioxidant Effects

scholarly journals Conifers Phytochemicals: A Valuable Forest with Therapeutic Potential

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 3005
Author(s):  
Kanchan Bhardwaj ◽  
Ana Sanches Silva ◽  
Maria Atanassova ◽  
Rohit Sharma ◽  
Eugenie Nepovimova ◽  
...  
Keyword(s):  
Experimental Data ◽  
Potential Role ◽  
Therapeutic Potential ◽  
Fungal Infections ◽  
Cell Damage ◽  
Cancer Growth ◽  
Naturally Occurring ◽  
Antioxidant Effects

Conifers have long been recognized for their therapeutic potential in different disorders. Alkaloids, terpenes and polyphenols are the most abundant naturally occurring phytochemicals in these plants. Here, we provide an overview of the phytochemistry and related commercial products obtained from conifers. The pharmacological actions of different phytochemicals present in conifers against bacterial and fungal infections, cancer, diabetes and cardiovascular diseases are also reviewed. Data obtained from experimental and clinical studies performed to date clearly underline that such compounds exert promising antioxidant effects, being able to inhibit cell damage, cancer growth, inflammation and the onset of neurodegenerative diseases. Therefore, an attempt has been made with the intent to highlight the importance of conifer-derived extracts for pharmacological purposes, with the support of relevant in vitro and in vivo experimental data. In short, this review comprehends the information published to date related to conifers’ phytochemicals and illustrates their potential role as drugs.

scholarly journals Antioxidant Effects and Cytoprotective Potentials of Herbal Tea against H2O2-Induced Oxidative Damage by Activating Heme Oxygenase1 Pathway

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Ke-Xin Zhang ◽  
Jian-Bin Tan ◽  
Cheng-Liang Xie ◽  
Rong-Bo Zheng ◽  
Xiao-Dan Huang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Protective Effect ◽  
Functional Foods ◽  
Cell Damage ◽  
Damage Model ◽  
Enrichment Analysis ◽  
Protective Effects ◽  
Herbal Tea ◽  
Antioxidant Effects

Herbal tea with antioxidant ingredients has gained increasing attention in the field of functional foods due to their amelioration potential in aging-related diseases. Wanglaoji herbal tea (WHT) is a kind of traditional beverage made from herbal materials. This study was performed to investigate its antioxidant activity and identify its protective effect on a H2O2-induced cell damage model. In this study, we identified six kinds of phenolic acids with antioxidant activity in WHT, among which rosmarinic acid had the highest content and the highest contribution ratio to the antioxidant activity of WHT. Moreover, compared with the H2O2-induced damage group, the WHT treatment group can significantly increase the viability of cells and decrease the ratio of senescence-associated β-galactosidase-positive cells, intracellular malondialdehyde levels, and the percentage of G1 phase. Furthermore, enrichment analysis of differentially expressed genes revealed that heme oxygenase1 (HMOX1) was a key gene for protective effect of WHT on oxidative stress-induced cell damage. Thus, WHT exerted protective effects not only by scavenging reactive oxygen species but also by inducing the expression of cytoprotective genes by activating the HMOX1 pathway, which showed that WHT had a potential of promoting health by reducing oxidative stress-induced cell damage.

scholarly journals HDL Inhibited Atherosclerosis Induced by Radiation Injury

2020 ◽  
Author(s):  
Jin Xie ◽  
Ke Zhu ◽  
Qingya Wang ◽  
Pei Zhao ◽  
Lihua Pan ◽  
...  
Keyword(s):  
Western Blotting ◽  
Cell Damage ◽  
Blood Lipid ◽  
Underlying Mechanism ◽  
Protective Role ◽  
Uvb Radiation ◽  
Aortic Endothelial Cells ◽  
Akt Phosphorylation ◽  
Apoptosis Protein ◽  
Apoptosis Process

Abstract Background- HDL inhibits atherosclerosis development from radiation damage, nonetheless, the underlying mechanism is yet to be defined.Methods-This study used radiation patients along with cultured mouse aortic endothelial cells (MAECs) to investigate the process. Firstly, 158 patients from the oncology department of Jingzhou hospital who received radiation after neck cancers participated, and their arterial function was monitored by B ultrasound. Similarly, HDL and other blood lipid indexes were also tested. Then, MAECs were isolated and cultured and passed through MTT assay to test the HDL protective role on UVB radiation along with western blotting to test some apoptosis protein expression and possible molecules.Results-Firstly, those patients with high HDL levels were less likely to develop atherosclerosis, with statistical differences. We observed that MAECs treated with UVB were damaged significantly however HDL reversed the cell damage in a dose-depended manner. In the meantime, the apoptosis process was assessed and found that HDL inhibited the apoptosis caused by UVB. Western blotting results showed that HDL enhanced phosphatidylinositol 3-kinase (PI3K) in addition to Akt phosphorylation in MAECs.Conclusion-These results suggest that HDL protected UVB-mediated apoptosis by activation of a mechanism involving PI3K/Akt signaling pathway.

scholarly journals MiR-664 Protects Against UVB Radiation-Induced HaCaT Cell Damage via Downregulating ARMC8

Dose-Response ◽  
2020 ◽  
Vol 18 (2) ◽  
pp. 155932582092923
Author(s):  
Chen Zhang ◽  
Xiongxiong Xie ◽  
Yawen Yuan ◽  
Yimeng Wang ◽  
Meijuan Zhou ◽  
...  
Keyword(s):  
Western Blot ◽  
Messenger Rna ◽  
Molecular Mechanisms ◽  
Cell Damage ◽  
Ultraviolet B ◽  
Cell Counting ◽  
Hacat Cells ◽  
Uvb Radiation ◽  
Related Factors ◽  
Radiation Induced

Background: MiR-664 has been demonstrated to play an important role in dermal diseases. However, the functions of miR-664 in ultraviolet B (UVB) radiation-induced keratinocytes damage remain to be elucidated. Objective: The present study aimed to investigate the molecular mechanisms under the UVB-induced keratinocytes damage and provide translational insights for future therapeutics and UVB protection. Methods: HaCaT cells were transfected with miR-664, either alone or combined with UVB irradiation. Levels of messenger RNA and protein were tested by quantitative real-time polymerase chain reaction and Western blot analyses. Cell proliferation, percentage of apoptotic cells, and expression levels of apoptosis-related factors were measured by Cell Counting Kit-8 assay, flow cytometry assay, and Western blot analysis, respectively. Results: We found that a significant increase in miR-664 was observed in UVB-induced HaCaT cells. Overexpressed miR-664 promoted cell vitalities and suppressed apoptosis of UVB-induced HaCaT cells. Additionally, the loss/gain of armadillo-repeat-containing protein 8 (ARMC8) rescued/blocked the effects of miR-664 on the proliferation of UVB-induced HaCaT cells. Conclusions: Our data demonstrate that miR-664 functions as a protective regulator in UVB-induced HaCaT cells via regulating ARMC8.

scholarly journals Changes in Lipid Profile of Keratinocytes from Rat Skin Exposed to Chronic UVA or UVB Radiation and Topical Application of Cannabidiol

Antioxidants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1178
Author(s):  
Wojciech Łuczaj ◽  
Maria do Rosário Domingues ◽  
Pedro Domingues ◽  
Elżbieta Skrzydlewska
Keyword(s):  
Oxidative Modification ◽  
Environmental Risk Factor ◽  
Uvb Radiation ◽  
Pla2 Activity ◽  
Rat Skin ◽  
Down Regulation ◽  
The Status ◽  
Antioxidant Effects ◽  
Phospholipid Profile

UV radiation is a well-established environmental risk factor known to cause oxidative stress and disrupt the metabolism of keratinocyte phospholipids. Cannabidiol (CBD) is a phytocannabinoid with anti-inflammatory and antioxidant effects. In this study, we examined changes in the keratinocyte phospholipid profile from nude rat skin exposed to UVA and UVB radiation that was also treated topically with CBD. UVA and UVB radiation promoted up-regulation of phosphatidylcholines (PC), lysophosphatidylcholines (LPC), phosphatidylethanolamines (PE) and down-regulation of sphingomyelin (SM) levels and enhanced the activity of phospholipase A2 (PLA2) and sphingomyelinase (SMase). Application of CBD to the skin of control rats led to down-regulation of SM and up-regulation of SMase activity. After CBD treatment of rats irradiated with UVA or UVB, SM was up-regulated and down-regulated, respectively, while ceramide (CER) levels and SMase activity were down-regulated and up-regulated, respectively. CBD applied to the skin of UV-irradiated rats down-regulated LPC, up-regulated PE and phosphatidylserines (PS) and reduced PLA2 activity. In conclusion, up-regulation of PS may suggest that CBD inhibits their oxidative modification, while changes in the content of PE and SM may indicate a role of CBD in promoting autophagy and improving the status of the transepidermal barrier.

scholarly journals Phototoxicity of nano titanium dioxides in HaCaT keratinocytes—Generation of reactive oxygen species and cell damage

2012 ◽  
Vol 263 (1) ◽  
pp. 81-88 ◽  
Author(s):  
Jun-Jie Yin ◽  
Jun Liu ◽  
Marilyn Ehrenshaft ◽  
Joan E. Roberts ◽  
Peter P. Fu ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Damage ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Hacat Keratinocytes ◽  
Titanium Dioxides

scholarly journals Pharmacological activation of TRPV4 produces immediate cell damage and induction of apoptosis in human melanoma cells and HaCaT keratinocytes

PLoS ONE ◽  
2018 ◽  
Vol 13 (1) ◽  
pp. e0190307 ◽  
Author(s):  
Aida Olivan-Viguera ◽  
Angel Luis Garcia-Otin ◽  
Javier Lozano-Gerona ◽  
Edgar Abarca-Lachen ◽  
Ana J. Garcia-Malinis ◽  
...  
Keyword(s):  
Cell Damage ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Hacat Keratinocytes ◽  
Human Melanoma Cells ◽  
Induction Of Apoptosis

scholarly journals The Antioxidant Effects of Calligonum Extract on Oxidative Stress in Spermatogonial Stem Cells Culture

2021 ◽  
Author(s):  
Shirin Barati ◽  
Mansoureh Movahedin
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Cell Damage ◽  
Antioxidant Property ◽  
Spermatogonial Stem Cells ◽  
Vital Role ◽  
Colonization Rate ◽  
Frap Assay ◽  
Cell Proliferation And Differentiation ◽  
Antioxidant Effects

Background: Spermatogenesis is a programmed route of germ cell proliferation and differentiation that could produce abundant numbers of spermatozoa. The antioxidants play a vital role in decreasing ROS production in cells, therefore plants extract administration with antioxidant property can prevent of cell damage. In the present study, antioxidant effects of Calligonum extract on proliferation and colonization rate of spermatogonial cells was assessed. Materials and Methods: After isolation and culture of spermatogonial stem cells (SSCs) from neonatal mice (4-5 day old) and identification by PLZF and Oct4 markers, the therapeutic effect of Calligonum comosum extract was measured on the cells treated with H2O2. Induced oxidative stress cells were treated with 10 μg/ml extract for 3 weeks. ROS levels were assessed by flow cytometry, proliferation by cell count and TAC by FRAP assay. Also, the apoptosis rate was measured with P53 and Bax genes by real time PCR method. Results: After three weeks of treatment, the Calligonum group showed significantly lower ROS level relative to the H2O2 group. Antioxidants levels were in Calligonum group significantly higher than the H2O2 group (P≤0.05). There was a strong inverse relationship between the two groups. Proliferation and colonization rate were in Calligonum + H2O2 group significantly higher than H2O2 group alone (P≤0.05). Finally, the results of the study indicated that P53 and Bax expression decreased in Calligonum + H2O2 group compared to H2O2 group. Conclusion: The results of present study expressed that Calligonum as a plant with antioxidant effect could reduce the level ofROS, and increase proliferation and colonization rate and TAC. In other hand, 30 μM doses of H2O2increased oxidative stress and apoptosiswhile decreased proliferation of SSCs.

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