In vitro toxicity of antimicrobials on RTG-2 and RTL-W1 fish cell lines

2005 ◽  
Vol 20 (1) ◽  
pp. 125-134 ◽  
Author(s):  
Mar Babín ◽  
Sara Boleas ◽  
José Vicente Tarazona
Keyword(s):  
Cell Lines ◽  
In Vitro Toxicity ◽  
Fish Cell ◽  
Fish Cell Lines

scholarly journals Isolation, Identification, and Genomic Analysis of a Novel Reovirus from Healthy Grass Carp and Its Dynamic Proliferation In Vitro and In Vivo

Viruses ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 690
Author(s):  
Ke Zhang ◽  
Wenzhi Liu ◽  
Yiqun Li ◽  
Yong Zhou ◽  
Yan Meng ◽  
...  
Keyword(s):  
Cell Lines ◽  
Grass Carp ◽  
Amino Acid Sequences ◽  
Gibel Carp ◽  
Fish Cell ◽  
Fish Cell Lines ◽  
Total Size ◽  
Sequence Comparisons ◽  
Grass Carp Reovirus

A new grass carp reovirus (GCRV), healthy grass carp reovirus (HGCRV), was isolated from grass carp in 2019. Its complete genome sequence was determined and contained 11 dsRNAs with a total size of 23,688 bp and 57.2 mol% G+C content, encoding 12 proteins. All segments had conserved 5' and 3' termini. Sequence comparisons showed that HGCRV was closely related to GCRV-873 (GCRV-I; 69.57–96.71% protein sequence identity) but shared only 22.65–45.85% and 23.37–43.39% identities with GCRV-HZ08 and Hubei grass carp disease reovirus (HGDRV), respectively. RNA-dependent RNA-polymerase (RdRp) protein-based phylogenetic analysis showed that HGCRV clustered with Aquareovirus-C (AqRV-C) prior to joining a branch common with other aquareoviruses. Further analysis using VP6 amino acid sequences from Chinese GCRV strains showed that HGCRV was in the same evolutionary cluster as GCRV-I. Thus, HGCRV could be a new GCRV isolate of GCRV-I but is distantly related to other known GCRVs. Grass carp infected with HGCRV did not exhibit signs of hemorrhage. Interestingly, the isolate induced a typical cytopathic effect in fish cell lines, such as infected cell shrank, apoptosis, and plague-like syncytia. Further analysis showed that HGCRV could proliferate in grass carp liver (L28824), gibel carp brain (GiCB), and other fish cell lines, reaching a titer of up to 7.5 × 104 copies/μL.

Erratum to “In vitro cytotoxicity testing of three zinc metal salts using established fish cell lines” [Toxicology in Vitro 18 (2004) 365–376]

2004 ◽  
Vol 18 (5) ◽  
pp. 731
Author(s):  
S Nı́ Shúilleabháin ◽  
C Mothersill ◽  
D Sheehan ◽  
N.M O'Brien ◽  
J O'Halloran ◽  
...  
Keyword(s):  
Cell Lines ◽  
In Vitro Cytotoxicity ◽  
Metal Salts ◽  
Fish Cell ◽  
Fish Cell Lines ◽  
Zinc Metal ◽  
Cytotoxicity Testing

scholarly journals Role and relevance of fish cell lines in advanced in vitro research

2022 ◽  
Author(s):  
M. Goswami ◽  
B. S. Yashwanth ◽  
Vance Trudeau ◽  
W. S. Lakra
Keyword(s):  
Cell Lines ◽  
Fish Cell ◽  
Fish Cell Lines

In vitro cytotoxicity testing of three zinc metal salts using established fish cell lines

2004 ◽  
Vol 18 (3) ◽  
pp. 365-376 ◽  
Author(s):  
S Nı́ Shúilleabháin ◽  
C Mothersill ◽  
D Sheehan ◽  
N.M O’Brien ◽  
J O’ Halloran ◽  
...  
Keyword(s):  
Cell Lines ◽  
In Vitro Cytotoxicity ◽  
Metal Salts ◽  
Fish Cell ◽  
Fish Cell Lines ◽  
Zinc Metal ◽  
Cytotoxicity Testing

scholarly journals In vitro assessment of the antimicrobial efficacy of chitosan nanoparticles against major fish pathogens and their cytotoxicity to fish cell lines

2020 ◽  
Vol 43 (9) ◽  
pp. 1049-1063 ◽  
Author(s):  
Fatma Ahmed ◽  
Faiza M. Soliman ◽  
Mohamed A. Adly ◽  
Hamdy A.M. Soliman ◽  
Mansour El‐Matbouli ◽  
...  
Keyword(s):  
Cell Lines ◽  
Chitosan Nanoparticles ◽  
Antimicrobial Efficacy ◽  
Fish Cell ◽  
Fish Cell Lines ◽  
Fish Pathogens ◽  
In Vitro Assessment

Comparative evaluation of the cytotoxicity sensitivity of six fish cell lines to four heavy metals in vitro

2008 ◽  
Vol 22 (1) ◽  
pp. 164-170 ◽  
Author(s):  
Fengxia Tan ◽  
Min Wang ◽  
Weimin Wang ◽  
Yuanan Lu
Keyword(s):  
Heavy Metals ◽  
Cell Lines ◽  
Comparative Evaluation ◽  
Fish Cell ◽  
Fish Cell Lines
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