Cloning, expression, and biochemical characterization of a novel NADP + -dependent 7α-hydroxysteroid dehydrogenase from Clostridium difficile and its application for the oxidation of bile acids

2017 ◽  
Vol 99 ◽  
pp. 16-24 ◽  
Author(s):  
Daniel Bakonyi ◽  
Werner Hummel
Keyword(s):  
Clostridium Difficile ◽  
Bile Acids ◽  
Biochemical Characterization ◽  
Hydroxysteroid Dehydrogenase

scholarly journals Structural and biochemical characterization of 20β-hydroxysteroid dehydrogenase fromBifidobacterium adolescentisstrain L2-32

2019 ◽  
Vol 294 (32) ◽  
pp. 12040-12053 ◽  
Author(s):  
Heidi L. Doden ◽  
Rebecca M. Pollet ◽  
Sean M. Mythen ◽  
Zdzislaw Wawrzak ◽  
Saravanan Devendran ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Hydroxysteroid Dehydrogenase

scholarly journals Biochemical Characterization of MarR and TetR Proteins from Clostridium difficile

2018 ◽  
Vol 32 (S1) ◽  
Author(s):  
Rei Takahashi ◽  
Bhumika Panchal ◽  
Da Eun Kim ◽  
Steven Wilkinson
Keyword(s):  
Clostridium Difficile ◽  
Biochemical Characterization

Biochemical Characterization of 17β-Hydroxysteroid Dehydrogenase in Human Meningioma

1984 ◽  
Vol 7 (4) ◽  
pp. 389-394
Author(s):  
P. M. Martin ◽  
A. M. Rieg ◽  
S. Fournier ◽  
H. Magdelenat ◽  
J. Hassoun ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Hydroxysteroid Dehydrogenase ◽  
Human Meningioma

Plant Pathology Diagnosed by X-Ray Microanalysis

1987 ◽  
Vol 45 ◽  
pp. 974-975
Author(s):  
J. H. Resau ◽  
N. Howell ◽  
S. H. Chang
Keyword(s):  
Electron Microscopy ◽  
Plant Pathology ◽  
Biochemical Characterization ◽  
Nutrient Deficiency ◽  
Green Leaf ◽  
Parasitic Infestation ◽  
X Ray

Spinach grown in Texas developed “yellow spotting” on the peripheral portions of the leaves. The exact cause of the discoloration could not be determined as there was no evidence of viral or parasitic infestation of the plants and biochemical characterization of the plants did not indicate any significant differences between the yellow and green leaf portions of the spinach. The present study was undertaken using electron microscopy (EM) to determine if a micro-nutrient deficiency was the cause for the discoloration.Green leaf spinach was collected from the field and sent by express mail to the EM laboratory. The yellow and equivalent green portions of the leaves were isolated and dried in a Denton evaporator at 10-5 Torr for 24 hrs. The leaf specimens were then examined using a JEOL 100 CX analytical microscope. TEM specimens were prepared according to the methods of Trump et al.

Interaction effect of rootstocks on gas exchange parameters, biochemical changes and nutrientstatus in Sauvignon Blanc winegrapes

2014 ◽  
Vol 3 (3) ◽  
pp. 218-225
Author(s):  
R. G. Somkuwar ◽  
M. A. Bhange ◽  
A. K. Upadhyay ◽  
S. D. Ramteke
Keyword(s):  
Biochemical Characterization ◽  
Reducing Sugar ◽  
Wine Grape ◽  
Gas Exchange Parameters ◽  
Food Material ◽  
Total Carbohydrates ◽  
Salt Creek ◽  
Grape Varieties ◽  
Photosynthetic Activities

SauvignonBlanc wine grape was characterized for their various morphological, physiological and biochemical parameters grafted on different rootstocks. Significant differences were recorded for all the parameters studied. The studies on vegetative parameters revealed that the rootstock influences the vegetative growth thereby increasing the photosynthetic activities of a vine. The highest photosynthesis rate was recorded in 140-Ru grafted vine followed by Fercal whereas the lowest in Salt Creek rootstock grafted vines.The rootstock influenced the changes in biochemical constituents in the grafted vine thereby helping the plant to store enough food material. Significant differences were recorded for total carbohydrates, proteins, total phenols and reducing sugar. The vines grafted on1103-Pshowed highest carbohydrates and starch followed by 140-Ru,while the least amount of carbohydrates were recorded in 110-R and Salt Creek grafted vines respectively.Among the different rootstock graft combinations, Fercal showed highest amount of reducing sugar, proteins and phenols, followed by 1103-P and SO4, however, the lowest amount of reducing sugar, proteins and phenols were recorded with 110-R grafted vines.The vines grafted on different rootstocks showed changes in nutrient uptake. Considering this, the physico-biochemical characterization of grafted vine may help to identify particularrootstocks combination that could influence a desired trait in commercial wine grape varieties after grafting.

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