CO2 reduction and organic compounds production by photosynthetic bacteria with surface displayed carbonic anhydrase and inducible expression of phosphoenolpyruvate carboxylase

2017 ◽  
Vol 96 ◽  
pp. 103-110 ◽  
Author(s):  
Ju-Yong Park ◽  
Yang-Hoon Kim ◽  
Jiho Min
Keyword(s):  
Carbonic Anhydrase ◽  
Organic Compounds ◽  
Phosphoenolpyruvate Carboxylase ◽  
Photosynthetic Bacteria ◽  
Co2 Reduction ◽  
Inducible Expression

Inhibition Profiling of Urease and Carbonic Anhydrase II by High- Throughput Screening and Molecular Docking Studies of Structurally Diverse Organic Compounds

2020 ◽  
Vol 17 ◽  
Author(s):  
Majid Ali ◽  
Syed Majid Bukhari ◽  
Asma Zaidi ◽  
Farhan A. Khan ◽  
Umer Rashid ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Carbonic Anhydrase ◽  
Metal Complexes ◽  
Organic Compounds ◽  
High Throughput ◽  
High Throughput Screening ◽  
Docking Studies ◽  
Carbonic Anhydrase Ii ◽  
Molecular Docking Studies ◽  
Ic50 Values

Background:: Structurally diverse organic compounds and available drugs were screened against urease and carbonic anhydrase II in a formulation acceptable for high-throughput screening. Objective: The study was conducted to find out potential inhibitors of urease and carbonic anhydrase II. Methods:: Quantification of the possible HITs was carried out by determining their IC50 values. Results and Discussion:: of several screened compounds including derivatives of oxadiazole, coumarins, chromane-2, 4- diones and metal complexes of cysteine-omeprazole showed promising inhibitory activities with IC50 ranging from 47 μM to 412 μM against the urease. The interactions of active compounds with active sites of enzymes were investigated through molecular docking studies which revealed that (R)-1-(4-amino-4-(5-(thiophen-2-yl)-1,3,4-oxadiazol-2-yl) butyl) guanidine possessing IC50 of 47 μM, interacts with one of the nickel metal atom of urease besides further interactions as predictable hydrogen bonds with KCX490, Asp633, His492, His407 and His409 along with Ala440 and 636. Bi-ligand metal complexes of 4-aminoantipyrine based Schiff bases showed activation of urease with AC50 ranging from 68 μM to 112 μM. Almost 21 compounds with varying functional groups including pyrimidines, oxadiazoles, imidazoles, hydrazides and tin based compounds were active carbonic anhydrase II inhibitors presenting 98 μM to 390 μM IC50 values. Several N-substituted sulfonamide derivatives were inactive against carbonic anhydrase II. Conclusion:: Among all the screened compounds, highly active inhibitor of carbonic anhydrase II was (4-(3- hydroxyphenyl)-6-phenyl-2-thioxo-1,2,3,4-tetrahydropyrimidin-5-yl)phenyl) methanone with IC50 of 98.0 μM. This particular compound showed metallic interaction with Zn ion of carbonic anhydrase II through hydroxyl group of phenyl ring.

scholarly journals Hypoxia-inducible expression of the mouse carbonic anhydrase IX demonstrated by new monoclonal antibodies

2007 ◽  
Author(s):  
Miriam Zatovicova ◽  
Silvia Pastorekova ◽  
Jaromir Pastorek ◽  
Seppo Parkkila ◽  
Alzbeta Hulikova ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Carbonic Anhydrase ◽  
Inducible Expression ◽  
Carbonic Anhydrase Ix

Carbon dioxide, the substrate for phosphoenolpyruvate carboxylase from leaves of maize

1969 ◽  
Vol 47 (9) ◽  
pp. 1455-1458 ◽  
Author(s):  
E. R. Waygood ◽  
Regis Mache ◽  
C. K. Tan
Keyword(s):  
Carbon Dioxide ◽  
Zea Mays ◽  
Carbonic Anhydrase ◽  
Phosphoenolpyruvate Carboxylase ◽  
Spinacea Oleracea ◽  
Natural Photosynthesis

Using a spectrophotometric technique it has been shown that CO2 is the substrate for phosphoenolpyruvate carboxylase (EC 4.1.1.31) from leaves of Zea mays. The Km (CO2) is approximately 7.5 × 10−6 M, which is the order of the half saturation value for CO2 in natural photosynthesis. Studies indicate also that carbonic anhydrase is localized in chloroplasts, isolated by the 'laceration technique' from leaves of Spinacea oleracea, but is absent from leaves of Zea mays. It is speculated that carbonic anhydrase catalyses a 'trap' for the CO2 escaping into the environment via photorespiration.

Conversion of carbon dioxide to oxaloacetate using integrated carbonic anhydrase and phosphoenolpyruvate carboxylase

2013 ◽  
Vol 36 (12) ◽  
pp. 1923-1928 ◽  
Author(s):  
Kwang Suk Chang ◽  
Hancheol Jeon ◽  
Man Bock Gu ◽  
Seung Pil Pack ◽  
EonSeon Jin
Keyword(s):  
Carbon Dioxide ◽  
Carbonic Anhydrase ◽  
Phosphoenolpyruvate Carboxylase

Immunolocalization of carbonic anhydrase and phosphoenolpyruvate carboxylase in developing seeds of Medicago sativa

2004 ◽  
Vol 42 (3) ◽  
pp. 181-186 ◽  
Author(s):  
Georgios Aivalakis ◽  
Maria Dimou ◽  
Emmanouil Flemetakis ◽  
Fotini Plati ◽  
Panagiotis Katinakis ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Medicago Sativa ◽  
Phosphoenolpyruvate Carboxylase ◽  
Developing Seeds

scholarly journals QSAR STUDIES OF SULPHONAMIDES AS CARBONIC ANHYDRASE II INHIBITORS USING TOPOLOGICAL INDICES

2019 ◽  
pp. 1-4
Author(s):  
Prabhanand Patel
Keyword(s):  
Carbonic Anhydrase ◽  
Organic Compounds ◽  
Parametric Model ◽  
Topological Indices ◽  
Carbonic Anhydrase Ii ◽  
Statistical Parameters ◽  
Qsar Studies

In this paper topological indices have been used in modeling log KI activity of 47 organic compounds acts as carbonic anhydrase II inhibitors. The 7-parametric model gave excellent results which contains mixed parameters. The results are discussed using different statistical parameters.

Siliceous Materials for Immobilization of Photoheterotrophic Bacteria

2011 ◽  
Vol 1326 ◽  
Author(s):  
Mikolaj Stodolny ◽  
Michal Thiel ◽  
Marcin Wlodarczak ◽  
Marek Laniecki
Keyword(s):  
Hydrochloric Acid ◽  
Surface Area ◽  
Organic Compounds ◽  
Photosynthetic Bacteria ◽  
Pore Diameter ◽  
Potato Starch ◽  
Sol Gel ◽  
Tetraethyl Orthosilicate ◽  
Commercial Potato ◽  
Vis Spectroscopy

ABSTRACTPreparation of transparent silica as potential carrier for photocatalysts and photosynthetic bacteria is presented. SiO2 was obtained by sol-gel techniques using tetraethyl orthosilicate as precursor. Glycol and glycerol were used as solvents. The increase of porosity of the studied materials was reached by application of soluble and commercial potato starch as a filler. Calcinations at 825 K for 6 hours removed all organic compounds. The rate of gelation was enhanced applying hydrochloric acid. Different materials were obtained in a series of syntheses with different concentrations of individual components. Transparency of the obtained materials varied between 50 and 97% (in range of λ 350 - 800 nm). Surface area varied between 300 and 400 m2/g and pore diameter was 5 – 18 nm. Samples were studied with SEM, nitrogen sorption at 77 K and UV-Vis spectroscopy. The obtained transparent materials with occluded TiO2 or CaTiO3 were tested in photocatalytic splitting of water.

Sign in / Sign up

Export Citation Format

Share Document