Electrospun polyvinyl alcohol/bovine serum albumin biocomposite membranes for horseradish peroxidase immobilization

2016 ◽  
Vol 93-94 ◽  
pp. 1-10 ◽  
Author(s):  
Ramin Fazel ◽  
Seyed-Fakhreddin Torabi ◽  
Pooya Naseri-Nosar ◽  
Salehe Ghasempur ◽  
Seyed-Omid Ranaei-Siadat ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Horseradish Peroxidase ◽  
Polyvinyl Alcohol ◽  
Serum Albumin ◽  
Bovine Serum

scholarly journals Heterologous screening of hybridomas for the development of broad-specific monoclonal antibodies against deoxynivalenol and its analogues

2014 ◽  
Vol 7 (3) ◽  
pp. 257-265 ◽  
Author(s):  
Y. Guo ◽  
M. Sanders ◽  
A. Galvita ◽  
A. Heyerick ◽  
D. Deforce ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Bovine Serum Albumin ◽  
Horseradish Peroxidase ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Simultaneous Detection ◽  
Cross Reactivity ◽  
Efficient Approach ◽  
Selection For ◽  
Similar Affinity

Hapten heterology was introduced into the steps of hybridoma selection for the development of monoclonal antibodies (MAbs) against deoxynivalenol (DON). Firstly, a novel heterologous DON hapten was synthesised and covalently coupled to proteins (i.e. bovine serum albumin (BSA), ovalbumin and horseradish peroxidase) using the linkage of cyanuric chloride (CC). After immunisation, antisera from different DON immunogens were checked for the presence of useful antibodies. Next, both homologous and heterologous enzyme-linked immunosorbent assays were conducted to screen for hybridomas. It was found that heterologous screening could significantly reduce the proportion of false positives and appeared to be an efficient approach for selecting hybridomas of interest. This strategy resulted in two kinds of broad-selective MAbs against DON and its analogues. They were quite distinct from other reported DON-antibodies in their cross-reactivity profiles. A unique MAb 13H1 derived from DON-CC-BSA immunogen could recognise DON and its analogues in the order of HT-2 toxin ≯ 15-acetyl-DON ≯ DON ≯ nivalenol, with IC50 ranging from 1.14 to 7.69 μg/ml. Another preferable MAb 10H10 generated from DON-BSA immunogen manifested relatively similar affinity to DON, 3-acetyl-DON and 15-acetyl-DON, with IC50 values of 22, 15 and 34 ng/ml, respectively. This is the first broad-specific MAb against DON and its two acetylated forms and thus it can be used for simultaneous detection of the three mycotoxins.

INFLUENCE OF IODOTHYRONINE CONJUGATES OF BOVINE SERUM ALBUMIN AND HORSERADISH PEROXIDASE ON ENZYME IMMUNOSORBENT ASSAY OF THYROID HORMONES

2013 ◽  
Vol 35 (2) ◽  
pp. 139-156
Author(s):  
G. Lakshmi Kumari ◽  
Sachin Kumar ◽  
Satish Gupta ◽  
Anuradha Saini ◽  
Sudesh K. Sharma ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Horseradish Peroxidase ◽  
Thyroid Hormones ◽  
Serum Albumin ◽  
Immunosorbent Assay ◽  
Bovine Serum

Effects of amino acid supplements and replacement of polyvinyl alcohol with bovine serum albumin in porcine zygote medium

2006 ◽  
Vol 18 (7) ◽  
pp. 789 ◽  
Author(s):  
Chie Suzuki ◽  
Koji Yoshioka
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Bovine Serum Albumin ◽  
Polyvinyl Alcohol ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Essential Amino Acids ◽  
Total Cell ◽  
Cell Numbers

The effects of glutamine, hypotaurine, taurine and premixed solutions of essential amino acids (EAA) and non-essential amino acids (NEAA) on in vitro development of porcine zygotes were evaluated. The effects of refreshing the medium and replacing polyvinyl alcohol (PVA) with bovine serum albumin (BSA) on embryonic development were also investigated. Porcine zygotes produced by in vitro maturation (IVM) and in vitro fertilisation (IVF) were cultured in porcine zygote medium (PZM), as the basal culture medium, for 5 days after IVF. The total number of cells in blastocysts was significantly increased by the addition of 2 mm glutamine to PZM, as was blastocyst yields after supplementation with 0.25 to 4 mm glutamine. Addition of 1.25 to 10 mm hypotaurine to PZM significantly increased blastocyst yields. Addition of 5 mm taurine to PZM significantly increased blastocyst yield, whereas taurine had no effect on blastocyst yield in cultures already containing 5 mm hypotaurine. Adding 1× EAA significantly increased the rate of blastocyst formation compared with no or 2× EAA, whereas 2× NEAA significantly increased the total cell numbers in blastocysts compared with no NEAA. Refreshing the medium at Day 3 had no effect on blastocyst yields, whereas medium change significantly reduced the total cell numbers in blastocysts. Adjusting the amino acid concentrations of a chemically defined medium can improve the developmental competence of porcine embryo.

Influence of Blocking Agents on Non-Specific Background of Polystyrene Microbeads in Serum Immunoassay

2013 ◽  
Vol 641-642 ◽  
pp. 858-861 ◽  
Author(s):  
Zhi Min Ren ◽  
Xi Nie ◽  
Sheng Shu Ai
Keyword(s):  
Bovine Serum Albumin ◽  
Polyvinyl Alcohol ◽  
Serum Albumin ◽  
Human Serum ◽  
Phosphate Buffer ◽  
Bovine Serum ◽  
Room Temperature ◽  
Tween 20 ◽  
Blocking Agents

In this paper, we used bovine serum albumin and polymer as the blocking agents and investigated the effect of blocking agents on non-specific background of polystyrene microbead that used the human serum immunoassay.The results showed that the nonspecific background is lower by using polymer blocking agents. The best blocking condition was that microbeads were blocked by PVXT (0.5% polyvinyl alcohol PVA, 0.8% polyvinylpyrrolidone, 0.05% Tween-20, PBS phosphate buffer, pH7.0) for two hours at room temperature.

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