Oxidative degradation of polyaromatic hydrocarbons catalyzed by blue laccase from Pleurotus ostreatus D1 in the presence of synthetic mediators

2006 ◽  
Vol 39 (6) ◽  
pp. 1242-1249 ◽  
Author(s):  
Natalia N. Pozdnyakova ◽  
Janina Rodakiewicz-Nowak ◽  
Olga V. Turkovskaya ◽  
Jerzy Haber
Keyword(s):  
Pleurotus Ostreatus ◽  
Polyaromatic Hydrocarbons ◽  
Oxidative Degradation ◽  
Pleurotus Ostreatus D1

Yellow laccase from the fungus Pleurotus ostreatus D1: Purification and characterization

2006 ◽  
Vol 42 (1) ◽  
pp. 56-61 ◽  
Author(s):  
N. N. Pozdnyakova ◽  
O. V. Turkovskaya ◽  
E. N. Yudina ◽  
Ya. Rodakiewicz-Nowak
Keyword(s):  
Pleurotus Ostreatus ◽  
Purification And Characterization ◽  
Yellow Laccase ◽  
Pleurotus Ostreatus D1

scholarly journals Novel Chloroflexi Genomes From The Deepest Ocean Reveal Metabolic Strategies For The Adaptation To Deep-Sea Habitats

2021 ◽  
Author(s):  
Rulong Liu ◽  
Xing Wei ◽  
Li Wang ◽  
Junwei Cao ◽  
Weizhi Song ◽  
...  
Keyword(s):  
Deep Sea ◽  
Current Knowledge ◽  
Polyaromatic Hydrocarbons ◽  
Oxidative Degradation ◽  
Deep Ocean ◽  
Metabolic Reconstruction ◽  
Regulatory Modules ◽  
Metabolic Plasticity ◽  
Wide Range ◽  
Metabolic Strategies

Abstract Background: The deep-sea harbors the majority of the microbial biomass in the Ocean, and it is a key site for organic matter (OM) remineralization and storage in the biosphere. Microbial metabolism in the deep ocean is greatly controlled by the generally depleted but periodically fluctuating supply of OM. Currently, little is known about metabolic potentials of dominant deep-sea microbes to cope with the variable OM inputs, especially for those living in the hadal trenches - the deepest part of the ocean. Results: In this study, we report the first extensive examination of the metabolic potentials of hadal sediment Chloroflexi, a dominant phylum in hadal trenches and the global deep ocean. Sixty-two metagenome-assembled-genomes (MAGs) were reconstructed from nine metagenomic datasets derived from sediments of the Mariana Trench. These MAGs represent six novel species, four novel genera, one novel family and one novel order within the classes Anaerolineae and Dehalococcoidia. Fragment recruitment showed that these MAGs are globally distributed in deep-sea waters and surface sediments, and transcriptomic analysis indicated their in-situ activities. Metabolic reconstruction showed that hadal Chloroflexi mainly had a heterotrophic lifestyle, with the potential to degrade a wide range of organic carbon, sulfur, and halogenated compounds. Our results revealed for the first time that hadal Chloroflexi harbor pathways for the complete hydrolytic or oxidative degradation of various recalcitrant OM, including aromatic compounds (e.g. benzoate), polyaromatic hydrocarbons (e.g. fluorene), polychlorobiphenyl (e.g. 4-chlorobiphenyl) and organochlorine compounds (e.g. chloroalkanes, chlorocyclohexane). Moreover, these organisms showed the potential to synthesize energy storage compounds (e.g. trehalose), and had regulatory modules to respond to changes in nutrient conditions. These metabolic traits lead us to postulate that the Chloroflexi may follow a “feast and famine” metabolic strategy, allowing them to efficiently consume labile OM and store the energy under OM rich conditions, and to survive under OM limitations by utilizing stored energy and degrading recalcitrant OM. Conclusion: This study expands the current knowledge on metabolic strategies in deep-ocean Chlorolfexi, and highlights their significance in deep-sea carbon, sulfur and halogen cycles. The metabolic plasticity likely provides Chloroflexi with advantages for the survival under variable and heterogenic OM inputs in the deep ocean.

Catalytic properties of yellow laccase from Pleurotus ostreatus D1

2004 ◽  
Vol 30 (1) ◽  
pp. 19-24 ◽  
Author(s):  
N.N Pozdnyakova ◽  
J Rodakiewicz-Nowak ◽  
O.V Turkovskaya
Keyword(s):  
Pleurotus Ostreatus ◽  
Catalytic Properties ◽  
Yellow Laccase ◽  
Pleurotus Ostreatus D1

Influence of cultivation conditions on pyrene degradation by the fungus Pleurotus Ostreatus D1

2009 ◽  
Vol 26 (2) ◽  
pp. 205-211 ◽  
Author(s):  
Natalia N. Pozdnyakova ◽  
Svetlana V. Nikiforova ◽  
Oleg E. Makarov ◽  
Marina P. Chernyshova ◽  
Kirill E. Pankin ◽  
...  
Keyword(s):  
Pleurotus Ostreatus ◽  
Cultivation Conditions ◽  
Pyrene Degradation ◽  
Pleurotus Ostreatus D1

Emulsifying Agent Production During PAHs Degradation by the White Rot Fungus Pleurotus Ostreatus D1

2009 ◽  
Vol 58 (6) ◽  
pp. 554-558 ◽  
Author(s):  
Svetlana V. Nikiforova ◽  
Natalia N. Pozdnyakova ◽  
Olga V. Turkovskaya
Keyword(s):  
Pleurotus Ostreatus ◽  
White Rot ◽  
White Rot Fungus ◽  
Emulsifying Agent ◽  
Pleurotus Ostreatus D1

Chrysene bioconversion by the white rot fungus Pleurotus ostreatus D1

Microbiology ◽  
2010 ◽  
Vol 79 (4) ◽  
pp. 456-460 ◽  
Author(s):  
S. V. Nikiforova ◽  
N. N. Pozdnyakova ◽  
O. E. Makarov ◽  
M. P. Chernyshova ◽  
O. V. Turkovskaya
Keyword(s):  
Pleurotus Ostreatus ◽  
White Rot ◽  
White Rot Fungus ◽  
Pleurotus Ostreatus D1

Influence of PAHs on ligninolytic enzymes of the fungus Pleurotus ostreatus D1

2010 ◽  
Vol 5 (1) ◽  
pp. 83-94 ◽  
Author(s):  
Natalia Pozdnyakova ◽  
Svetlana Nikiforova ◽  
Olga Turkovskaya
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Pleurotus Ostreatus ◽  
Degradation Products ◽  
Ligninolytic Enzymes ◽  
Versatile Peroxidase ◽  
Cultivation Medium ◽  
Polycyclic Aromatic ◽  
The Difference ◽  
Pleurotus Ostreatus D1

AbstractPolycyclic aromatic hydrocarbons (PAHs), their derivatives, and their degradation products were assayed for the ability to enhance activities of ligninolytic enzymes (laccase and versatile peroxidase) of the fungus Pleurotus ostreatus D1. The activities of both laccase and versatile peroxidase were induced by the PAHs, their derivatives, and their degradation products. Laccase was produced mostly in the first 7–10 days, whereas the production of versatile peroxidase began after 5–7 days of cultivation. Non-denaturing PAGE showed the presence of additional forms of laccase and versatile peroxidase in the presence of the xenobiotics in the cultivation medium. The difference in the production time for these enzymes may reflect that laccases are involved in the first stages of PAHs degradation and that versatile peroxidase can be necessary for oxidation of some degradation products. This is the first report on versatile peroxidase induction by PAHs and their derivatives.

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