Effects of endocrine disrupting chemicals on estrogen receptor alpha and heat shock protein 60 gene expression in primary cultures of loggerhead sea turtle (Caretta caretta) erythrocytes

2017 ◽  
Vol 158 ◽  
pp. 616-624 ◽  
Author(s):  
Paolo Cocci ◽  
Martina Capriotti ◽  
Gilberto Mosconi ◽  
Francesco Alessandro Palermo
Keyword(s):  
Gene Expression ◽  
Estrogen Receptor ◽  
Estrogen Receptor Alpha ◽  
Primary Cultures ◽  
Endocrine Disrupting Chemicals ◽  
Caretta Caretta ◽  
Sea Turtle ◽  
Heat Shock Protein 60 ◽  
Loggerhead Sea Turtle ◽  
Endocrine Disrupting

scholarly journals Differentiation of PC12 cells expressing estrogen receptor alpha: A new bioassay for endocrine-disrupting chemicals evaluation

Chemosphere ◽  
2014 ◽  
Vol 112 ◽  
pp. 240-247 ◽  
Author(s):  
Denis Habauzit ◽  
François Ferrière ◽  
Nadine Botherel ◽  
Gilles Flouriot ◽  
Farzad Pakdel ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Pc12 Cells ◽  
Estrogen Receptor Alpha ◽  
Endocrine Disrupting Chemicals ◽  
Receptor Alpha ◽  
Endocrine Disrupting ◽  
New Bioassay

scholarly journals Tissue and Temperature-Specific RNA-Seq Analysis Reveals Genomic Versatility and Adaptive Potential in Wild Sea Turtle Hatchlings (Caretta caretta)

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3013
Author(s):  
Julie C. Chow ◽  
Nia Kyritsis ◽  
Micah Mills ◽  
Matthew H. Godfrey ◽  
Craig A. Harms ◽  
...  
Keyword(s):  
Gene Expression ◽  
Differentially Expressed Genes ◽  
Sexual Development ◽  
Caretta Caretta ◽  
Sea Turtle ◽  
Differentially Expressed ◽  
Specific Gene ◽  
Rna Seq ◽  
Loggerhead Sea Turtle ◽  
Male And Female

Background: Digital transcriptomics is rapidly emerging as a powerful new technology for modelling the environmental dynamics of the adaptive landscape in diverse lineages. This is particularly valuable in taxa such as turtles and tortoises (order Testudines) which contain a large fraction of endangered species at risk due to anthropogenic impacts on the environment, including pollution, overharvest, habitat degradation, and climate change. Sea turtles (family Cheloniidae) in particular invite a genomics-enabled approach to investigating their remarkable portfolio of adaptive evolution. The sex of the endangered loggerhead sea turtle (Caretta caretta) is subject to temperature-dependent sex determination (TSD), a mechanism by which exposure to temperatures during embryonic development irreversibly determines sex. Higher temperatures produce mainly female turtles and lower temperatures produce mainly male turtles. Incubation temperature can have long term effects on the immunity, migratory ability, and ultimately longevity of hatchlings. We perform RNA-seq differential expression analysis to investigate tissue- and temperature-specific gene expression within brain (n = 7) and gonadal (n = 4) tissue of male and female loggerhead hatchlings. Results: We assemble tissue- and temperature-specific transcriptomes and identify differentially expressed genes relevant to sexual development and life history traits of broad adaptive interest to turtles and other amniotic species. We summarize interactions among differentially expressed genes by producing network visualizations, and highlight shared biological pathways related to migration, immunity, and longevity reported in the avian and reptile literature. Conclusions: The measurement of tissue- and temperature-specific global gene expression of an endangered, flagship species such as the loggerhead sea turtle (Caretta caretta) reveals the genomic basis for potential resiliency and is crucial to future management and conservation strategies with attention to changing climates. Brain and gonadal tissue collected from experimentally reared loggerhead male and female hatchlings comprise an exceedingly rare dataset that permits the identification of genes enriched in functions related to sexual development, immunity, longevity, and migratory behavior and will serve as a large, new genomic resource for the investigation of genotype–phenotype relationships in amniotes.

Aeromonas induced polyostotic osteomyelitis in a juvenile loggerhead sea turtle Caretta caretta

2018 ◽  
Vol 132 (1) ◽  
pp. 79-84 ◽  
Author(s):  
A Pace ◽  
L Meomartino ◽  
A Affuso ◽  
G Mennonna ◽  
S Hochscheid ◽  
...  
Keyword(s):  
Caretta Caretta ◽  
Sea Turtle ◽  
Loggerhead Sea Turtle

scholarly journals Repression of ESR1 through Actions of Estrogen Receptor Alpha and Sin3A at the Proximal Promoter

2009 ◽  
Vol 29 (18) ◽  
pp. 4949-4958 ◽  
Author(s):  
Stephanie J. Ellison-Zelski ◽  
Natalia M. Solodin ◽  
Elaine T. Alarid
Keyword(s):  
Gene Expression ◽  
Estrogen Receptor ◽  
Transcription Factor ◽  
Rna Polymerase Ii ◽  
Transcriptional Repression ◽  
Estrogen Receptor Alpha ◽  
Proximal Promoter ◽  
Receptor Alpha ◽  
Expression Of Genes ◽  
Activation Of Transcription

ABSTRACT Gene expression results from the coordinated actions of transcription factor proteins and coregulators. Estrogen receptor alpha (ERα) is a ligand-activated transcription factor that can both activate and repress the expression of genes. Activation of transcription by estrogen-bound ERα has been studied in detail, as has antagonist-induced repression, such as that which occurs by tamoxifen. How estrogen-bound ERα represses gene transcription remains unclear. In this report, we identify a new mechanism of estrogen-induced transcriptional repression by using the ERα gene, ESR1. Upon estrogen treatment, ERα is recruited to two sites on ESR1, one distal (ENH1) and the other at the proximal (A) promoter. Coactivator proteins, namely, p300 and AIB1, are found at both ERα-binding sites. However, recruitment of the Sin3A repressor, loss of RNA polymerase II, and changes in histone modifications occur only at the A promoter. Reduction of Sin3A expression by RNA interference specifically inhibits estrogen-induced repression of ESR1. Furthermore, an estrogen-responsive interaction between Sin3A and ERα is identified. These data support a model of repression wherein actions of ERα and Sin3A at the proximal promoter can overcome activating signals at distal or proximal sites and ultimately decrease gene expression.

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