Effects of crude oil on phospholipid fatty acid compositions of marine hydrocarbon degraders: estimation of the bacterial membrane fluidity

2005 ◽  
Vol 97 (3) ◽  
pp. 300-311 ◽  
Author(s):  
N. Mazzella ◽  
A.D. Syakti ◽  
J. Molinet ◽  
M. Gilewicz ◽  
P. Doumenq ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Crude Oil ◽  
Membrane Fluidity ◽  
Phospholipid Fatty Acid ◽  
Bacterial Membrane ◽  
Fatty Acid Compositions ◽  
Hydrocarbon Degraders

Diet-induced alterations in the discoid shape and phospholipid fatty acid compositions of rat erythrocytes

Lipids ◽  
1979 ◽  
Vol 14 (1) ◽  
pp. 30-38 ◽  
Author(s):  
G. Ananda Rao ◽  
Kathleen Siler ◽  
Edward C. Larkin
Keyword(s):  
Fatty Acid ◽  
Phospholipid Fatty Acid ◽  
Rat Erythrocytes ◽  
Fatty Acid Compositions

scholarly journals Effects of Constant and Diel Cyclic Temperatures on The Liver and Intestinal Phospholipid Fatty Acid Composition in Rainbow Trout Oncorhynchus Mykiss During Seawater Acclimation

2021 ◽  
Author(s):  
Jian Ge ◽  
Yangen Zhou ◽  
Ming Huang ◽  
Qinfeng Gao ◽  
Yunwei Dong ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Rainbow Trout ◽  
Membrane Fluidity ◽  
Phospholipid Fatty Acid ◽  
Principal Component ◽  
Average Chain Length ◽  
Phospholipid Fatty Acid Composition ◽  
Cyclic Temperature ◽  
Seawater Acclimation ◽  
Growth Trial

Abstract Background: Rainbow trout is an important economic fish in aquaculture and a model species in environmental physiology. Despite of earlier research on the seawater adaptability of rainbow trout at different temperature regimes, the influence on liver and intestine in this species is still unknown. Two trials were conducted to investigate the effects of constant and diel cyclic temperatures on phospholipid fatty acid (PLFA) composition in the liver and intestine of rainbow trout during seawater acclimation.Results: At the end of the growth trial 1, fish at 9 and 12.5°C showed significantly higher ratios of unsaturated to saturated (U/S) and unsaturation index (UI) than those at 16°C in liver and intestine phospholipids. After day 1 of seawater acclimation, the U/S, UI, and average chain length (ACL) of liver and intestine phospholipids in fish at 16°C significantly increased. Two weeks after seawater acclimation, liver and intestinal PLFA composition adapted to salinity change. In trial 2, significantly higher U/S, UI, and ACL were found in intestinal phospholipids at 13±2°C. On the first day after seawater acclimation, UI and ACL in liver phospholipids significantly increased in 13°C, while fish in 13±2°C showed significantly decreased U/S, UI, and ACL in the intestine. At the end of the growth trial 2, liver PLFA compositions were stable, whereas intestinal PLFA in 13 and 13±1°C showed significantly decreased U/S, UI, and ACL. A two-way analysis of variance and principal component analysis revealed significant effects of different constant temperatures, seawater acclimation, and their interaction on the liver and intestinal phospholipids, a significant effect of diel cyclic temperature on intestinal phospholipids, and the effects of seawater acclimation and its interaction with diel cyclic temperature on liver phospholipids.Conclusion: Temperatures of 9 and 12.5°C could elevate membrane fluidity and thickness in the liver and intestine of rainbow trout in freshwater, whereas no significant effects were found with diel temperature variations. After seawater acclimation, constant and diel cyclic temperatures significantly influenced the liver and intestine's membrane fluidity and thickness. Compared with constant temperature, diel temperature variation (13±2°C) can enhance the adaptability of rainbow trout during seawater acclimation.

Community dynamics of a mixed-bacterial culture growing on petroleum hydrocarbons in batch culture

2000 ◽  
Vol 46 (5) ◽  
pp. 441-450 ◽  
Author(s):  
Jonathan D Van Hamme ◽  
Joseph A Odumeru ◽  
Owen P Ward
Keyword(s):  
Community Structure ◽  
Fatty Acid ◽  
Crude Oil ◽  
Batch Culture ◽  
Petroleum Hydrocarbons ◽  
Community Dynamics ◽  
Heterotrophic Bacteria ◽  
Phospholipid Fatty Acid ◽  
Fatty Acid Analysis ◽  
Phospholipid Fatty Acid Analysis

The effects of various hydrocarbon substrates, and a chemical surfactant capable of enhancing crude-oil biodegradation, on the community structure of a mixed-bacterial inoculum were examined in batch culture. Of 1000 TSA-culturable isolates, 68.6% were identified at the genus level or better by phospholipid fatty acid analysis over 7-day time course experiments. Cultures were exposed to 20 g/L Bow River crude oil with and without 0.625 g/L Igepal CO-630 (a nonylphenol ethoxylate surfactant), 5 g/L saturates, 5 g/L aromatics, or 125 g/L refinery sludge. A group of six genera dominated the cultures: Acinetobacter, Alcaligenes, Ochrobactrum, Pseudomonas/Flavimonas, Stenotrophomonas, and Yersinia. Species from four of the genera were shown to be capable of hydrocarbon degradation, and counts of hydrocarbon degrading and total heterotrophic bacteria over time were nearly identical. Pseudomonas/Flavimonas and Stenotrophomonas normally dominated during the early portions of cultures, although the lag phase of Stenotrophomonas appears to have been increased by surfactant addition. Acinetobacter calcoaceticus was the most frequently isolated microorganism during exposure to the saturate fraction of crude oil. Regardless of substrate, the culture medium supported a greater variety of organisms during the latter portions of cultures. Understanding the community structure and dynamics of mixed bacterial cultures involved in treatment of heterogeneous waste substrates may assist in process development and optimization studies.Key words: mixed culture, phospholipid fatty acid analysis, petroleum hydrocarbons, community structure.

Sign in / Sign up

Export Citation Format

Share Document