scholarly journals Comparative study of Cu uptake and early transcriptome responses in the green microalga Chlamydomonas reinhardtii and the macrophyte Elodea nuttallii

2019 ◽  
Vol 250 ◽  
pp. 331-337 ◽  
Author(s):  
Rebecca Beauvais-Flück ◽  
Vera I. Slaveykova ◽  
Claudia Cosio
Keyword(s):  
Comparative Study ◽  
Chlamydomonas Reinhardtii ◽  
Elodea Nuttallii ◽  
Green Microalga ◽  
Cu Uptake

scholarly journals Introns mediate post-transcriptional enhancement of nuclear gene expression in the green microalga Chlamydomonas reinhardtii

PLoS Genetics ◽  
2020 ◽  
Vol 16 (7) ◽  
pp. e1008944 ◽  
Author(s):  
Thomas Baier ◽  
Nick Jacobebbinghaus ◽  
Alexander Einhaus ◽  
Kyle J. Lauersen ◽  
Olaf Kruse
Keyword(s):  
Gene Expression ◽  
Chlamydomonas Reinhardtii ◽  
Nuclear Gene ◽  
Nuclear Gene Expression ◽  
Green Microalga

scholarly journals Comparative study of phototactic and photophobic receptor chromophore properties in Chlamydomonas reinhardtii

1993 ◽  
Vol 65 (1) ◽  
pp. 508-518 ◽  
Author(s):  
D.N. Zacks ◽  
F. Derguini ◽  
K. Nakanishi ◽  
J.L. Spudich
Keyword(s):  
Comparative Study ◽  
Chlamydomonas Reinhardtii

Functional analysis of three type-2 DGAT homologue genes for triacylglycerol production in the green microalga Chlamydomonas reinhardtii

2012 ◽  
Vol 162 (1) ◽  
pp. 13-20 ◽  
Author(s):  
M. La Russa ◽  
C. Bogen ◽  
A. Uhmeyer ◽  
A. Doebbe ◽  
E. Filippone ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Chlamydomonas Reinhardtii ◽  
Green Microalga

scholarly journals Orfamide-A-mediated bacterial-algal interactions involve specific Ca2+ signalling pathways

2021 ◽  
Author(s):  
Yu Hou ◽  
Yuko Bando ◽  
David Carrasco Flores ◽  
Vivien Susann Hotter ◽  
Bastian Schiweck ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Trp Channels ◽  
Signalling Pathways ◽  
Antagonistic Bacterium ◽  
Cyclic Lipopeptide ◽  
Green Microalga

The antagonistic bacterium Pseudomonas protegens secretes the cyclic lipopeptide orfamide A, which triggers a Ca2+ signal, causing the deflagellation of the green microalga Chlamydomonas reinhardtii. By investigating targeted synthetic orfamide A variants and inhibitors, we found that at least two Ca2+-signalling pathways and TRP channels are involved in this response.

scholarly journals The Path to Triacylglyceride Obesity in the sta6 Strain of Chlamydomonas reinhardtii

2014 ◽  
Vol 13 (5) ◽  
pp. 591-613 ◽  
Author(s):  
Ursula Goodenough ◽  
Ian Blaby ◽  
David Casero ◽  
Sean D. Gallaher ◽  
Carrie Goodson ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Growth Conditions ◽  
Culture Conditions ◽  
Pentose Phosphate ◽  
Small Subset ◽  
Content Type ◽  
Green Microalga ◽  
Genes Encoding ◽  
Encoding Genes ◽  
Tag Accumulation

ABSTRACT When the sta6 (starch-null) strain of the green microalga Chlamydomonas reinhardtii is nitrogen starved in acetate and then “boosted” after 2 days with additional acetate, the cells become “obese” after 8 days, with triacylglyceride (TAG)-filled lipid bodies filling their cytoplasm and chloroplasts. To assess the transcriptional correlates of this response, the sta6 strain and the starch-forming cw15 strain were subjected to RNA-Seq analysis during the 2 days prior and 2 days after the boost, and the data were compared with published reports using other strains and growth conditions. During the 2 h after the boost, ∼425 genes are upregulated ≥2-fold and ∼875 genes are downregulated ≥2-fold in each strain. Expression of a small subset of “sensitive” genes, encoding enzymes involved in the glyoxylate and Calvin-Benson cycles, gluconeogenesis, and the pentose phosphate pathway, is responsive to culture conditions and genetic background as well as to boosting. Four genes—encoding a diacylglycerol acyltransferase ( DGTT2 ), a glycerol-3-P dehydrogenase ( GPD3 ), and two candidate lipases (Cre03.g155250 and Cre17.g735600)—are selectively upregulated in the sta6 strain. Although the bulk rate of acetate depletion from the medium is not boost enhanced, three candidate acetate permease-encoding genes in the GPR1/FUN34/YaaH superfamily are boost upregulated, and 13 of the “sensitive” genes are strongly responsive to the cell's acetate status. A cohort of 64 autophagy-related genes is downregulated by the boost. Our results indicate that the boost serves both to avert an autophagy program and to prolong the operation of key pathways that shuttle carbon from acetate into storage lipid, the combined outcome being enhanced TAG accumulation, notably in the sta6 strain.

scholarly journals Effect of overexpression of LPAAT and GPD1 on lipid synthesis and composition in green microalga Chlamydomonas reinhardtii

2017 ◽  
Vol 30 (3) ◽  
pp. 1711-1719 ◽  
Author(s):  
Chaogang Wang ◽  
Yi Li ◽  
Jun Lu ◽  
Xu Deng ◽  
Hui Li ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Lipid Synthesis ◽  
Green Microalga
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