Kinetics of nutrient enhanced crude oil degradation by Pseudomonas aeruginosa AKS1 and Bacillus sp. AKS2 isolated from Guwahati refinery, India

2016 ◽  
Vol 216 ◽  
pp. 548-558 ◽  
Author(s):  
Bobby Chettri ◽  
Arghya Mukherjee ◽  
James S. Langpoklakpam ◽  
Dhrubajyoti Chattopadhyay ◽  
Arvind K. Singh
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Crude Oil ◽  
Oil Degradation ◽  
Bacillus Sp ◽  
Crude Oil Degradation ◽  
Kinetics Of

scholarly journals Efficiency and Kinetics of Assam Crude Oil Degradation by Pseudomonas Aeruginosa AKS1 and Bacillus Sp. AKS2

2021 ◽  
Author(s):  
Bobby Chettri ◽  
Ningombam Anjana Singha ◽  
Arvind Kumar Singh
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Crude Oil ◽  
Liquid Medium ◽  
Half Life ◽  
Liquid Culture ◽  
Oil Degradation ◽  
Biodegradation Rate ◽  
Emulsification Index ◽  
Crude Oil Degradation ◽  
Kinetics Of

Abstract We report kinetics of Assam crude oil degradation by Pseudomonas aeruginosa AKS1 and Bacillus sp. AKS2, both isolated from Assam refinery sediments. The isolates exhibited appreciable degrees of hydrophobicity, emulsification index and biosurfactant production. Crude oil degradation efficiency of isolates was assessed in (1) liquid medium amended with 1% v/v crude oil and (2) microcosm sediments (125 mg crude oil/ 10 g sand). In liquid culture, the biodegradation rate (k) and half-life (t1/2) values were found to be 0.0383 day -1 and 18.09 days for P. aeruginosa AKS1, and 0.0204 day -1 and 33.97 days in case of Bacillus sp. AKS2. In microcosm sand sediments, the estimated biodegradation rate (k) and half-life (t 1/2) values were 0.0138 day -1 and 50 days for P. aeruginosa AKS1, and 0.0113 day -1 and 61.34 days in case of Bacillus sp. AKS2. The level of nutrient treatment in microcosm sand sediment was 125 µg N & 62.5 µg P/g sediment in case of P. aeruginosa AKS1 and 375 µg N & 37.5 µg P/g sediment in case of Bacillus sp. AKS2. In microcosms without inorganic nutrients, biodegradation rate (k) and half-life (t1/2) values were found to be 0.0069 day -1 and 100 days for P. aeruginosa AKS1 and for Bacillus sp. AKS2, the respective values were found to be 0.0046 day -1 and 150.68 days. Our data provides important information for predictive hydrocarbon degradation in liquid medium and contaminated sediments.

Transcriptome analysis of crude oil degrading Pseudomonas aeruginosa strains for identification of potential genes involved in crude oil degradation

Gene ◽  
2020 ◽  
Vol 755 ◽  
pp. 144909 ◽  
Author(s):  
Dhrubajyoti Das ◽  
Gabriella T. Mawlong ◽  
Yogita N. Sarki ◽  
Anil Kumar Singh ◽  
Channakeshavaiah Chikkaputtaiah ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Crude Oil ◽  
Transcriptome Analysis ◽  
Oil Degradation ◽  
Crude Oil Degradation

scholarly journals Variability in Pseudomonas aeruginosa Lipopolysaccharide Expression during Crude Oil Degradation

2002 ◽  
Vol 68 (10) ◽  
pp. 5096-5103 ◽  
Author(s):  
R. Sean Norman ◽  
Roberto Frontera-Suau ◽  
Pamela J. Morris
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Cell Surface ◽  
Crude Oil ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Cell Surface Hydrophobicity ◽  
Colony Morphology ◽  
Oil Degradation ◽  
O Antigen ◽  
Crude Oil Degradation

ABSTRACT Bacterial utilization of crude oil components, such as the n-alkanes, requires complex cell surface adaptation to allow adherence to oil. To better understand microbial cell surface adaptation to growth on crude oil, the cell surface characteristics of two Pseudomonas aeruginosa strains, U1 and U3, both isolated from the same crude oil-degrading microbial community enriched on Bonny Light crude oil (BLC), were compared. Analysis of growth rates demonstrated an increased lag time for U1 cells compared to U3 cells. Amendment with EDTA inhibited U1 and U3 growth and degradation of the n-alkane component of BLC, suggesting a link between cell surface structure and crude oil degradation. U1 cells demonstrated a smooth-to-rough colony morphology transition when grown on BLC, while U3 cells exhibited rough colony morphology at the outset. Combining high-resolution atomic force microscopy of the cell surface and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of extracted lipopolysaccharides (LPS), we demonstrate that isolates grown on BLC have reduced O-antigen expression compared with that of glucose-grown cells. The loss of O-antigen resulted in shorter LPS molecules, increased cell surface hydrophobicity, and increased n-alkane degradation.

scholarly journals Complete genome sequence analysis of Pseudomonas aeruginosa N002 reveals its genetic adaptation for crude oil degradation

Genomics ◽  
2015 ◽  
Vol 105 (3) ◽  
pp. 182-190 ◽  
Author(s):  
Dhrubajyoti Das ◽  
Reshita Baruah ◽  
Abhijit Sarma Roy ◽  
Anil Kumar Singh ◽  
Hari Prasanna Deka Boruah ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Sequence Analysis ◽  
Crude Oil ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Genetic Adaptation ◽  
Oil Degradation ◽  
Genome Sequence Analysis ◽  
Crude Oil Degradation ◽  
Complete Genome Sequence Analysis

Methanogenic crude oil degradation induced by an exogenous microbial community and nutrient injections

2021 ◽  
Vol 201 ◽  
pp. 108458
Author(s):  
Konomi Suda ◽  
Masayuki Ikarashi ◽  
Hideyuki Tamaki ◽  
Satoshi Tamazawa ◽  
Susumu Sakata ◽  
...  
Keyword(s):  
Microbial Community ◽  
Crude Oil ◽  
Oil Degradation ◽  
Crude Oil Degradation
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