Copper-induced response of physiological parameters and antioxidant enzymes in the aquatic macrophyte Potamogeton pusillus

2009 ◽  
Vol 157 (8-9) ◽  
pp. 2570-2576 ◽  
Author(s):  
Magdalena V. Monferrán ◽  
José A. Sánchez Agudo ◽  
María L. Pignata ◽  
Daniel A. Wunderlin
Keyword(s):  
Antioxidant Enzymes ◽  
Aquatic Macrophyte ◽  
Physiological Parameters ◽  
Induced Response ◽  
Potamogeton Pusillus

scholarly journals The relationship of antioxidant enzymes and some physiological parameters in maize during chilling

2011 ◽  
Vol 50 (No. 1) ◽  
pp. 27-32 ◽  
Author(s):  
T. Takáč
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Glutathione Reductase ◽  
Ascorbate Peroxidase ◽  
Reductase Activity ◽  
Physiological Parameters ◽  
Maize Seedlings ◽  
Maize Cultivars ◽  
Inhibition Of Growth ◽  
Glutathione Reductase Activity

The changes in some physiological parameters of maize seedlings in response to chilling were studied. The emphasis was laid upon their relationship to chilling induced alterations in antioxidant enzymes (superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase) activity. The exposure of maize seedlings to chilling caused substantial defects in the 4-day-old seedlings and the seedlings with two fully developed leaves, respectively. The membrane semipermeability perturbations and the loss of viability in the young seedlings were observed. Similarly, we found a decrease of chlorophyll content, appearance of necrotic lesions and inhibition of growth in older plants. The measurements of superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase activities provide an evidence of reactive oxygen species formation, that is assumed to be a reason of the found damages. Significant differences between two cultivars were found in the studied parameters. The electrolyte leakage and viability test provided effective methods for the characterization of the chilling tolerance-level in maize cultivars.

scholarly journals Results of the use of a sorbent in a carp feed with signs of mycotoxin contamination

2021 ◽  
pp. 72-86
Author(s):  
О. Deren ◽  
◽  
N. Syrovatka ◽  
M. Koryliak ◽  
О. Dobryanska ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Physiological Parameters ◽  
Feed Additives ◽  
Control Group ◽  
Average Weight ◽  
Defense System ◽  
Diene Conjugates

Purpose. The aim of the work was to study certain morpho-physiological parameters of carp as a result of additional introduction of a mycotoxin sorbent to a feed with signs of spoilage. Methodology. During 2019–2020, two experiments were conducted in the laboratory. The first one included 7 groups-analogues of age-1+ Nyvky carp, 20 fish in each group, with an average weight of 22.5 g. The control group of fish was fed for 24 days with a compound feed with signs of spoilage, «Mykosorb» was additionally added to the diet of the experimental groups in the amounts of 0.05, 0.1; 0.15; 0.2; 0.25 and 0.4%, respectively. The second group included 4 groups of age-1+ fish, 12 fish in each group, with an average weight of 20.0 g. The diet of the control group was identical to that of the first experiment, and the experimental groups with the addition of 0.05, 0.075 and 0.1% of «Mycosorb», respectively. During the study, optimal conditions for keeping fish were provided. Fish after were measured and weighed at the end of the experiment and their weight gains were analyzed according to generally accepted methods in fish farming. The activity of antioxidant enzymes and the content of lipid peroxidation products depending on the composition of the diet were studied. Findings. The studies used compound feed with signs of spoilage as a result of violation of their storage technology, which identified three groups of toxins: Aflatoxin B1, Zearalenone and Deoxynivalenol. As a result of the first stage of the study, the rates of application of mycotoxin sorbent into the diet of carp at amounts of 0.05 - 0.2% were determined. At the same time, there was no clear proportional pattern of changes in the average weight of fish depending on the amount of introduction of «Mycosorb», the value increased in all experimental groups compared to the control by 0.4 – 15.2%. Muscles of age-1+ fish were found to have higher (p<0,001) catalase activity after application of 0.15% of the test drug by 1.4 times, 0.2% — by 46%, 0.25% — by 69 and 0.4% — by 79%. Superoxide dismutase activity also increased, except for the groups of 0.2, 0.25 and 0.4% of «Mycosorb». There was a tendency of 21.0 and 22.6% decrease in the content of diene conjugates as a result of feeding 0.05 and 0.1% sorbent and some increase as a result of feeding 0.15; 0.2; 0.25 and 0.4% additives. Studies of the next stage showed that addition of «Mykosorb» at the rate of 0.05, 0.075 and 0.1% to the feed, the average weight of age-1+ carp in the experimental groups increased by 45.4; 37.2 and 30.3%, the condition factor — by 41.9; 40.8 and 42.7%, the relative growth rate is 4.3; 5.9 and 4.6 times, respectively. The fish body height to length ratio was lower by 10.3, 7.0 and 7.4% relative to the control group. Addition of 0.05, 0.075 and 0.1% of «Mycosorb» increased resistance of fish due to an increase in the hepatopancreatic activity of antioxidant enzymes: superoxide dismutase by 28.3, 30.5 and 5.0% and catalase by 56.5 (p<0.001), 55.5 (p<0.001) and 46.3% (p<0.05). At the same time, addition of 0.05 and 0.1% of «Mykosorb» increased the content of diene conjugates by 11.3 (p<0.01) and 12.3% (p<0.01), TBC-active products by 19.7 (p<0.05) and 13.2%. Originality. For the first time, the feasibility of addition of the mycotoxin sorbent «Mykosorb» into the feed with signs of spoilage was studied. The effect of feeding age-1+ carp with diets of various compositions on some morpho-physiological parameters of carp in the conditions of model tests was carried out. Practical value. Additional weigh gains and improvements of the antioxidant defense system of the carp organism were observed. The use of «Mykosorb» in carp feeding allows reducing the negative impact of low quality feed on fish body. Key words: carp, feed, feed additives, mycotoxins, sorbent, morphological parameters, growth, antioxidant defense system

Enhanced phytoextraction of chromium by the aquatic macrophyte Potamogeton pusillus in presence of copper

2012 ◽  
Vol 161 ◽  
pp. 15-22 ◽  
Author(s):  
Magdalena V. Monferrán ◽  
María L. Pignata ◽  
Daniel A. Wunderlin
Keyword(s):  
Aquatic Macrophyte ◽  
Potamogeton Pusillus ◽  
Enhanced Phytoextraction

Effects of Antioxidant Supplementation and Exercise Training on Erythrocyte Antioxidant Enzymes

2006 ◽  
Vol 76 (5) ◽  
pp. 324-331 ◽  
Author(s):  
Marsh ◽  
Laursen ◽  
Coombes
Keyword(s):  
Antioxidant Enzymes ◽  
Exercise Training ◽  
Endurance Training ◽  
Young Male ◽  
Antioxidant Defenses ◽  
Antioxidant Enzyme Activities ◽  
Antioxidant Supplementation ◽  
Male Wistar Rats ◽  
Exercise Induced ◽  
Antioxidant Supplements

Erythrocytes transport oxygen to tissues and exercise-induced oxidative stress increases erythrocyte damage and turnover. Increased use of antioxidant supplements may alter protective erythrocyte antioxidant mechanisms during training. Aim of study: To examine the effects of antioxidant supplementation (α-lipoic acid and α-tocopherol) and/or endurance training on the antioxidant defenses of erythrocytes. Methods: Young male Wistar rats were assigned to (1) sedentary; (2) sedentary and antioxidant-supplemented; (3) endurance-trained; or (4) endurance-trained and antioxidant-supplemented groups for 14 weeks. Erythrocyte superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT) activities, and plasma malondialdehyde (MDA) were then measured. Results: Antioxidant supplementation had no significant effect (p > 0.05) on activities of antioxidant enzymes in sedentary animals. Similarly, endurance training alone also had no effect (p > 0.05). GPX (125.9 ± 2.8 vs. 121.5 ± 3.0 U.gHb–1, p < 0.05) and CAT (6.1 ± 0.2 vs. 5.6 ± 0.2 U.mgHb–1, p < 0.05) activities were increased in supplemented trained animals compared to non-supplemented sedentary animals whereas SOD (61.8 ± 4.3 vs. 52.0 ± 5.2 U.mgHb–1, p < 0.05) activity was decreased. Plasma MDA was not different among groups (p > 0.05). Conclusions: In a rat model, the combination of exercise training and antioxidant supplementation increased antioxidant enzyme activities (GPX, CAT) compared with each individual intervention.

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