scholarly journals Insights into Cronobacter sakazakii Biofilm Formation and Control Strategies in the Food Industry

Engineering ◽  
2020 ◽  
Vol 6 (4) ◽  
pp. 393-405 ◽  
Author(s):  
Na Ling ◽  
Stephen Forsythe ◽  
Qingping Wu ◽  
Yu Ding ◽  
Jumei Zhang ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Food Industry ◽  
Control Strategies ◽  
Cronobacter Sakazakii ◽  
And Control

Understanding of food biofilms by the application of omics techniques

2021 ◽  
Author(s):  
Lei Yuan ◽  
Fedrick C Mgomi ◽  
Zhenbo Xu ◽  
Ni Wang ◽  
Guoqing He ◽  
...  
Keyword(s):  
Food Safety ◽  
Foodborne Pathogens ◽  
Food Processing ◽  
Biofilm Formation ◽  
Food Industry ◽  
Processing Conditions ◽  
Protective Barrier ◽  
And Control

Biofilms constitute a protective barrier for foodborne pathogens to survive under stressful food processing conditions. Therefore, studies into the development and control of biofilms by novel techniques are vital for the food industry. In recent years, foodomics techniques have been developed for biofilm studies, which contributed to a better understanding of biofilm behavior, physiology, composition, as well as their response to antibiofilm methods at different molecular levels including genes, RNA, proteins and metabolic metabolites. Throughout this review, the main studies where foodomics tools used to explore the mechanisms for biofilm formation, dispersal and elimination were reviewed. The data summarized from relevant studies are important to design novel and appropriate biofilm elimination methods for enhancing food safety at any point of food processing lines.

scholarly journals Biofilm formation and control strategies of foodborne pathogens: food safety perspectives

RSC Advances ◽  
2017 ◽  
Vol 7 (58) ◽  
pp. 36670-36683 ◽  
Author(s):  
Xihong Zhao ◽  
Fenghuan Zhao ◽  
Jun Wang ◽  
Nanjing Zhong
Keyword(s):  
Food Safety ◽  
Foodborne Pathogens ◽  
Biofilm Formation ◽  
Control Strategies ◽  
Food Poisoning ◽  
Foodborne Diseases ◽  
Main Factors ◽  
And Control

Foodborne pathogens are the main factors behind foodborne diseases and food poisoning and thus pose a great threat to food safety.

scholarly journals Inhibition of Cronobacter Sakazakii Biofilm Formation and Expression of Virulence Factors by Coenzyme Q0

2020 ◽  
Author(s):  
Ning Guan ◽  
Yiqi Shi ◽  
Haoyu Tong ◽  
Yanpeng Yang ◽  
Jiahui Li ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Laser Scanning ◽  
Coenzyme Q ◽  
Polymerase Chain Reaction Analysis ◽  
Laser Scanning Microscopy ◽  
Raw 264.7 Cells ◽  
Confocal Laser ◽  
Dependent Manner ◽  
Cronobacter Sakazakii ◽  
And Control

Abstract Objectives：Here, we investigated the inhibitory effects of coenzyme Q0 (CoQ0) on biofilm formation and the expression of virulence genes by Cronobacter sakazakii. Results：We found that the minimum inhibitory concentration of CoQ0 against C. sakazakii strains ATCC29544 and ATCC29004 was 100 μg/mL, while growth curve assays showed that sub-inhibitory concentrations (SICs) of CoQ0 for both strains were 6.4, 3.2, 1.6 and 0.8 μg/mL. Assays exploring the inhibition of specific biofilm formation showed that SICs of CoQ0 inhibited biofilm formation by C. sakazakii in a dose-dependent manner, which was confirmed by scanning electron microscopy and confocal laser scanning microscopy analyses. CoQ0 inhibited the swimming and swarming motility of C. sakazakii and reduced its ability to adhere to and invade HT-29 cells. In addition, CoQ0 impeded the ability of C. sakazakii to survive and replicate within RAW 264.7 cells. Finally, real time polymerase chain reaction analysis confirmed that nine C. sakazakii genes associated with biofilm formation and virulence were down-regulated in response to CoQ0 treatment. Conclusion：Overall, our findings suggest that CoQ0 is a promising antibiofilm agent and provide new insights for the prevention and control of infections caused by C. sakazakii.

Efficacy of organic peroxyacids for eliminating biofilm preformed by microorganisms isolated from dairy processing plants

2021 ◽  
Author(s):  
Coralie Goetz ◽  
Jules Larouche ◽  
Maribel Velez Aristizabal ◽  
Nissa Niboucha ◽  
Julie Jean
Keyword(s):  
Biofilm Formation ◽  
Food Industry ◽  
Dynamic Method ◽  
Negative Impact ◽  
Control Strategies ◽  
Dairy Industry ◽  
Single Species ◽  
Dairy Processing ◽  
Processing Plants

The aim of this study was to evaluate the ability of microorganisms isolated from the dairy industry to form biofilms and to investigate the efficacity of organic peroxyacids (peracetic, perpropionic and perlactic acids and BioDestroy®) to eradicate those biofilms. Eighteen microorganisms were isolated from Quebec dairy processing plants that have issues associated with biofilm formation and were presumptively identified by MALDI-TOF mass spectrometry. The single-species biofilm-producing ability of the isolates was then evaluated using 96-well microplates. Eight out of eighteen (8/18) of these isolates were identified as moderate or strong biofilm producers, and ten out of eighteen (10/18) resulted as negative or weak biofilm producers. The efficacy of above-mentioned disinfectants was tested on the stronger biofilm producing bacteria using the MBEC (Minimum Biofilm Eradication Concentration) assay. After 5 min, all the disinfectants tested successfully eradicated both the single and mixed biofilms when applied following the recommended concentration. However, the efficacy of organic peroxyacids was significantly variable at lower concentrations. For example, 25 ppm of BioDestroy® were sufficient to eradicate all the biofilms, except for Pseudomonas azotoformans PFl1A. Unfortunately, microscopic observations highlighted those dead cells were still attached to the surfaces. In conclusion, our results suggest that some microorganisms found in dairy plants can produce tenacious biofilms that are, however, still susceptible to disinfectants, including organic peroxyacids. Further studies would be needed in order to confirm these observations using a dynamic method to mimic in vivo conditions. IMPORTANCE Biofilm forming microorganisms are a major issue in the food industry, including dairy industry, because of their negative impact on products quality. Biofilms are difficult to remove by clean-in-place (CIP) procedures commonly used in processing plants and may be less sensitive to sanitizers. Therefore, it is important to identify these microorganisms, in order to develop biofilm control strategies. The results gathered in the present study could contribute to this aim, even though it was carried out using only static methods.

scholarly journals Listeria monocytogenes Biofilms in the Food Industry: Is the Current Hygiene Program Sufficient to Combat the Persistence of the Pathogen?

2021 ◽  
Vol 9 (1) ◽  
pp. 181
Author(s):  
Tina Mazaheri ◽  
Brayan R. H. Cervantes-Huamán ◽  
Maria Bermúdez-Capdevila ◽  
Carolina Ripolles-Avila ◽  
José Juan Rodríguez-Jerez
Keyword(s):  
Listeria Monocytogenes ◽  
Food Industry ◽  
Safety Management ◽  
Control Strategies ◽  
Critical Evaluation ◽  
Standardized Protocol ◽  
Industry Environment ◽  
Processing Plants ◽  
And Control

Biofilms contain microbial cells which are protected by a self-produced matrix and they firmly attach themselves to many different food industry surfaces. Due to this protection, microorganisms within biofilms are much more difficult to eradicate and therefore to control than suspended cells. A bacterium that tends to produce these structures and persist in food processing plants is Listeria monocytogenes. To this effect, many attempts have been made to develop control strategies to be applied in the food industry, although there seems to be no clear direction on how to manage the risk the bacteria poses. There is no standardized protocol that is applied equally to all food sectors, so the strategies for the control of this pathogen depend on the type of surface, the nature of the product, the conditions of the food industry environment, and indeed the budget. The food industry performs different preventive and corrective measures on possible L. monocytogenes-contaminated surfaces. However, a critical evaluation of the sanitization methods applied must be performed to discern whether the treatment can be effective in the long-term. This review will focus on currently used strategies to eliminate biofilms and control their formation in processing facilities in different food sectors (i.e., dairy, meat, fish, chilled vegetables, and ready-to-eat products). The technologies employed for their control will be exemplified and discussed with the objective of understanding how L. monocytogenes can be improved through food safety management systems.

Investigate of the Ability of Cronobacter sakazakii Isolated from Clinical Samples of Children Under Two Years to Induce Swimming, Swarming and Biofilm

2018 ◽  
pp. 123
Author(s):  
Luma Abdal Hady Zwein ◽  
Tharieyt Abdulrahman Motlag ◽  
Mohamed Mousa
Keyword(s):  
Cerebrospinal Fluid ◽  
Congo Red ◽  
Biofilm Formation ◽  
Microtiter Plate ◽  
Clinical Samples ◽  
Cronobacter Sakazakii ◽  
Yellow Color ◽  
Golden Yellow ◽  
Vitek 2 ◽  
Biochemical Examination

      The study included 200 samples were collected   from   children  under two   years included (50 samples from each of Cerebrospinal fluid, Blood, Stool and Urine) from, Central Children Hospital and Children's Protections Educational Hospital. Isolates bacterial were obtained cultural, microscopic and biochemical examination and diagnosed to the species by using vitek2 system. The results showed there were contamination in 6.5% of clinical samples. The diagnosed colonies which gave pink color on the MacConkey agar , golden yellow color on the Trypton Soy agar and green color on the Birillent Enterobacter sakazakii agar and gave  a probability of 99% in the vitek 2 and were identified as Cronobacter sakazakii. The identification revealed of thirteen isolates: 6(46.16%) isolated from Cerebrospinal fluid samples, 7(53.84%) isolated from blood samples and not isolated bacteria from stool and urine samples. The results of the investigation of some virulence factors showed that all bacteria isolates were able to swimming with a diameter ranging (1-9 mm) and swarming with a diameter ranging (1-40 mm) and their  ability to biofilm formation  by using three methods. The results show the ability  of  isolates to form biofilm by using  Congo red media  methods where it is 12 (92.30 %) out of 13 isolated bacteria belonging to C. sakazakii  able to form biofilm on the Congo red media  which is 3 (23.07%) were  strong production  biofilm ,   8 (61.53%)  were intermediate  production  biofilm and  1 (7.69% ) were weak  biofilm formation , while the 1 (7.69%)  unable to form biofilm.  Tubes method were all isolates were able to form biofilm, it were found that 3 (23.07%)  isolates strong, and 8 (61.53%) intermediate  and 2( 15.38%)  weak biofilm formation. Microtiter plate method  gave 5 (38.46 %) isolates strong, 6 (46.15%) intermediate and 1 (7.69%) weak biofilm formation.  

Effective infection prevention and control strategies in a large accredited psychiatric facility in Singapore

2020 ◽  
Author(s):  
Daniel Poremski ◽  
Sandra Henrietta Subner ◽  
Grace Lam Fong Kin ◽  
Raveen Dev Ram Dev ◽  
Mok Yee Ming ◽  
...  
Keyword(s):  
Mental Health ◽  
Prevention And Control ◽  
Infection Prevention ◽  
Control Strategies ◽  
Infection Prevention And Control ◽  
Psychiatric Facility ◽  
Essential Services ◽  
Community Transmission ◽  
And Control

The Institute of Mental Health in Singapore continues to attempt to prevent the introduction of COVID-19, despite community transmission. Essential services are maintained and quarantine measures are currently unnecessary. To help similar organizations, strategies are listed along three themes: sustaining essential services, preventing infection, and managing human and consumable resources.

Sign in / Sign up

Export Citation Format

Share Document