Bioelectrochemistry and enzymatic activity of glucose oxidase immobilized onto the bamboo-shaped CNx nanotubes

2005 ◽  
Vol 51 (4) ◽  
pp. 611-618 ◽  
Author(s):  
Nengqin Jia ◽  
Ling Liu ◽  
Qin Zhou ◽  
Lijun Wang ◽  
Manming Yan ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Glucose Oxidase

Glucose oxidase-directed, instant synthesis of Mn-doped ZnS quantum dots in neutral media with retained enzymatic activity: mechanistic study and biosensing application

2015 ◽  
Vol 3 (29) ◽  
pp. 5942-5950 ◽  
Author(s):  
Jinyi Zhang ◽  
Airu Zhu ◽  
Ting Zhao ◽  
Lan Wu ◽  
Peng Wu ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Enzymatic Activity ◽  
Glucose Oxidase ◽  
Mechanistic Study ◽  
Directed Synthesis ◽  
Neutral Media ◽  
Mn Doped ◽  
Target Analyte

Retained enzymatic activity in glucose oxidase-directed synthesis of QDs was achieved, in which the enzyme acts not only as a ligand for synthesis of QDs, but also bridges the QDs with the target analyte.

Tuning the redox and enzymatic activity of glucose oxidase in layered organic films and its application in glucose biosensors

2004 ◽  
Vol 329 (1) ◽  
pp. 85-90 ◽  
Author(s):  
Wenjun Zhang ◽  
Yinxi Huang ◽  
Han Dai ◽  
Xiaoyue Wang ◽  
Chunhai Fan ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Glucose Oxidase ◽  
Organic Films ◽  
Glucose Biosensors

Enzymatic activity of glucose oxidase encapsulated in transparent glass by the sol-gel method

1992 ◽  
Vol 4 (3) ◽  
pp. 495-497 ◽  
Author(s):  
Stacey A. Yamanaka ◽  
Fumito Nishida ◽  
Lisa M. Ellerby ◽  
Clinton R. Nishida ◽  
Bruce Dunn ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Glucose Oxidase ◽  
Sol Gel ◽  
Transparent Glass ◽  
Gel Method ◽  
Sol Gel Method

The folding and enzymatic activity of glucose oxidase in the glycolipid matrixes of different charges

1999 ◽  
Vol 352 (1-2) ◽  
pp. 213-217 ◽  
Author(s):  
Jin-ru Li ◽  
Yu-ke Du ◽  
P Boullanger ◽  
L Jiang
Keyword(s):  
Enzymatic Activity ◽  
Glucose Oxidase

Electrostatic Immobilization of Glucose Oxidase in a Weak Acid, Polyelectrolyte Hyperbranched Ultrathin Film on Gold:  Fabrication, Characterization, and Enzymatic Activity

1999 ◽  
Vol 71 (15) ◽  
pp. 3133-3139 ◽  
Author(s):  
Justine G. Franchina ◽  
William M. Lackowski ◽  
Daniel L. Dermody ◽  
Richard M. Crooks ◽  
David E. Bergbreiter ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Glucose Oxidase ◽  
Weak Acid ◽  
Ultrathin Film

scholarly journals A background-free assay for cells forming antibody to glucose oxidase coupled with an enzyme-linked immunosorbent assay (ELISA) to quantitate antibody secretion.

1989 ◽  
Vol 37 (6) ◽  
pp. 909-912 ◽  
Author(s):  
R C Hard ◽  
W G Miller ◽  
G Romagnoli
Keyword(s):  
Enzymatic Activity ◽  
Glucose Oxidase ◽  
Immunosorbent Assay ◽  
Individual Cell ◽  
Plaque Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Large Numbers ◽  
Mammalian Tissues ◽  
Immunocytochemical Assay ◽  
Antibody Secretion

We describe an immunocytochemical assay for cells forming antibody to glucose oxidase (GO). The method is specific in that only cells containing intracytoplasmic antibody capable of binding the immunogen (GO binding cells; GOBC) are stained. The method is sensitive because there is no GO activity in mammalian tissues. This lack of background readily permits detection of one GOBC among 10(6) nucleated lymphohemopoietic cells. The technique is reliable because purified chemicals are used. Although it is not possible to determine the Ig class of antibody formed by an individual cell, as can be done with the hemolytic plaque assay, the amount and class of secreted antibody to GO can be quantitated by an indirect enzyme-linked immunosorbent assay (ELISA), which is also described. GO is immunogenic and stimulates the formation of large numbers of GOBC in the popliteal lymph nodes after injection with adjuvant into the footpads of mice, but 1-mg doses injected IV or IP are lethal because of its enzymatic activity, which causes hypoglycemia and methemoglobinemia.

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