scholarly journals Electrochemistry of a high redox potential laccase obtained by computer-guided mutagenesis combined with directed evolution

2019 ◽  
Vol 106 ◽  
pp. 106511 ◽  
Author(s):  
Olga Aleksejeva ◽  
Ivan Mateljak ◽  
Roland Ludwig ◽  
Miguel Alcalde ◽  
Sergey Shleev
Keyword(s):  
Redox Potential ◽  
Directed Evolution ◽  
High Redox Potential

scholarly journals Switching from blue to yellow: altering the spectral properties of a high redox potential laccase by directed evolution

2012 ◽  
Vol 31 (1) ◽  
pp. 8-21 ◽  
Author(s):  
Diana M. Mate ◽  
Eva Garcia-Ruiz ◽  
Susana Camarero ◽  
Vladimir V. Shubin ◽  
Magnus Falk ◽  
...  
Keyword(s):  
Redox Potential ◽  
Directed Evolution ◽  
Spectral Properties ◽  
High Redox Potential

scholarly journals Expression system of CotA-laccase for directed evolution and high-throughput screenings for the oxidation of high-redox potential dyes

2009 ◽  
Vol 4 (4) ◽  
pp. 558-563 ◽  
Author(s):  
Vânia Brissos ◽  
Luciana Pereira ◽  
Florentina-Daniela Munteanu ◽  
Artur Cavaco-Paulo ◽  
Lígia O. Martins
Keyword(s):  
Redox Potential ◽  
Directed Evolution ◽  
High Throughput ◽  
Expression System ◽  
Cota Laccase ◽  
High Redox Potential

A few key residues determine the high redox potential shift in azurin mutants

2015 ◽  
Vol 13 (45) ◽  
pp. 11003-11013 ◽  
Author(s):  
Laura Zanetti-Polzi ◽  
Carlo A. Bortolotti ◽  
Isabella Daidone ◽  
Massimiliano Aschi ◽  
Andrea Amadei ◽  
...  
Keyword(s):  
Redox Potential ◽  
Potential Shift ◽  
Key Residues ◽  
High Redox Potential

The changes in the redox potential of Azurin upon mutation stem from the effects of a few key residues, including non-mutated ones, rather than being the result of a generalized rearrangement.

scholarly journals Purification and Partial Characterization of a Thermostable Laccase from Pycnoporus sanguineus CS-2 with Ability to Oxidize High Redox Potential Substrates and Recalcitrant Dyes

10.5772/56374 ◽  
2013 ◽  
Author(s):  
Sergio M. Salcedo Martnez ◽  
Guadalupe Gutirrez-Soto ◽  
Carlos F. Rodriguez Garza ◽  
Tania J. Villarreal Galvan ◽  
Juan F. Contreras Cordero ◽  
...  
Keyword(s):  
Redox Potential ◽  
Partial Characterization ◽  
Pycnoporus Sanguineus ◽  
High Redox Potential

scholarly journals Titanium-based potassium-ion battery positive electrode with extraordinarily high redox potential

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Stanislav S. Fedotov ◽  
Nikita D. Luchinin ◽  
Dmitry A. Aksyonov ◽  
Anatoly V. Morozov ◽  
Sergey V. Ryazantsev ◽  
...  
Keyword(s):  
Redox Potential ◽  
Potassium Ion ◽  
Positive Electrode ◽  
High Redox Potential

scholarly journals Increasing Redox Potential, Redox Mediator Activity, and Stability in a Fungal Laccase by Computer-Guided Mutagenesis and Directed Evolution

ACS Catalysis ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 4561-4572 ◽  
Author(s):  
Ivan Mateljak ◽  
Emanuele Monza ◽  
Maria Fatima Lucas ◽  
Victor Guallar ◽  
Olga Aleksejeva ◽  
...  
Keyword(s):  
Redox Potential ◽  
Directed Evolution ◽  
Redox Mediator ◽  
Fungal Laccase

The effect of pH and complexation on redox reactions between RS• radicals and flavins

1984 ◽  
Vol 62 (1) ◽  
pp. 171-177 ◽  
Author(s):  
Rizwan Ahmad ◽  
David A. Armstrong
Keyword(s):  
Redox Potential ◽  
Linear Function ◽  
Redox Reactions ◽  
Flavin Adenine Dinucleotide ◽  
Protonated Form ◽  
Redox Properties ◽  
The Other ◽  
Effect Of Ph ◽  
Other Hand ◽  
High Redox Potential

Elementary considerations indicate that thiol radicals, RS•, should have a high redox potential [Formula: see text][Formula: see text]However, the equilibrium [4],[Formula: see text]which is established in the presence of excess RS−, would convert RS•to [Formula: see text] which is a reducing species. Experimentally it was demonstrated that thiol radicals made by γ radiolysis of β-mercaptoethanol solutions effected two-electron oxidation of dihydroflavin FlH2 at pH 6.3 and of FlH− at pH 8. On the other hand, [Formula: see text] readily reduced Fl to FlH2 or FlH− as expected. At pH 9, photostationary states were established after a few minutes radiolysis and the ratios [FlH−]ss/[Fl]ss were a function of [Formula: see text] The main reactions occurring were:[Formula: see text]The values of k19 and k22 were both large. The ratio k19/k22 was ∼0.8 for lumiflavin and ∼0.3 for flavin adenine dinucleotide. The cyclic disulphide anions of lipoamide and dithiothreitol [Formula: see text] also effected two-electron reductions of flavins. However, the protonated form of [Formula: see text] oxidized FlH2, and the photostationary ratio [FlH−]ss/[Fl]ss was an approximate linear function of [Formula: see text]. The implications of the observed changes in redox properties of sulphur radicals on complexation with RS− and protonation were briefly considered.Des considérations élémentaires indiquent que les radicaux thiyles, RS•, doivent avoir un potentiel rédox élevé [Formula: see text][Formula: see text]

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