Changes in the intrinsic electrocatalytic nature of Na+/K+ ATPase reflect structural changes on ATP-binding: Electrochemical label-free approach

2013 ◽  
Vol 27 ◽  
pp. 104-107 ◽  
Author(s):  
Jan Vacek ◽  
Martina Zatloukalova ◽  
Marika Havlikova ◽  
Jitka Ulrichova ◽  
Martin Kubala
Keyword(s):  
Structural Changes ◽  
Atp Binding ◽  
Label Free ◽  
Electrochemical Label

scholarly journals Label-free imaging of age-related cardiac structural changes in non-human primates using multiphoton nonlinear microscopy

2021 ◽  
Author(s):  
Amara Khan ◽  
Fernanda Ramos-Gomes ◽  
Andrea Markus ◽  
Matthias Mietsch ◽  
Rabea Hinkel ◽  
...  
Keyword(s):  
Structural Changes ◽  
Nonlinear Microscopy ◽  
Label Free ◽  
Age Related

scholarly journals Zebrafish structural development in Mueller-matrix scanning microscopy

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Aymeric Le Gratiet ◽  
Marta d’Amora ◽  
Marti Duocastella ◽  
Riccardo Marongiu ◽  
Artemi Bendandi ◽  
...  
Keyword(s):  
Structural Changes ◽  
Molecular Mechanisms ◽  
Developmental Stages ◽  
Mueller Matrix ◽  
Fluorescence Probes ◽  
Imaging Method ◽  
Structural Development ◽  
Matrix Formalism ◽  
Label Free ◽  
Biological Organization

AbstractZebrafish are powerful animal models for understanding biological processes and the molecular mechanisms involved in different human diseases. Advanced optical techniques based on fluorescence microscopy have become the main imaging method to characterize the development of these organisms at the microscopic level. However, the need for fluorescence probes and the consequent high light doses required to excite fluorophores can affect the biological process under observation including modification of metabolic function or phototoxicity. Here, without using any labels, we propose an implementation of a Mueller-matrix polarimeter into a commercial optical scanning microscope to characterize the polarimetric transformation of zebrafish preserved at different embryonic developmental stages. By combining the full polarimetric measurements with statistical analysis of the Lu and Chipman mathematical decomposition, we demonstrate that it is possible to quantify the structural changes of the biological organization of fixed zebrafish embryos and larvae at the cellular scale. This convenient implementation, with low light intensity requirement and cheap price, coupled with the quantitative nature of Mueller-matrix formalism, can pave the way for a better understanding of developmental biology, in which label-free techniques become a standard tool to study organisms.

scholarly journals ATP binding promotes light-induced structural changes to the protein moiety of Arabidopsis cryptochrome 1

2020 ◽  
Vol 19 (10) ◽  
pp. 1326-1331
Author(s):  
Tatsuya Iwata ◽  
Daichi Yamada ◽  
Katsuhiro Mikuni ◽  
Kazuya Agata ◽  
Kenichi Hitomi ◽  
...  
Keyword(s):  
Structural Changes ◽  
Proton Donor ◽  
Atp Binding ◽  
Cryptochrome 1 ◽  
Protein Moiety

D396 is protonated and acts as a proton donor to FAD in Arabidopsis cryptochome 1 only when ATP is bound.

scholarly journals A Graphene-Based Glycan Biosensor for Electrochemical Label-Free Detection of a Tumor-Associated Antibody

Sensors ◽  
2019 ◽  
Vol 19 (24) ◽  
pp. 5409 ◽  
Author(s):  
Filip Kveton ◽  
Anna Blsakova ◽  
Lenka Lorencova ◽  
Monika Jerigova ◽  
Dusan Velic ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Secondary Ion Mass Spectrometry ◽  
Covalent Immobilization ◽  
Label Free ◽  
Surface Plasmon Resonance Analysis ◽  
Binding Preference ◽  
Electrochemical Label ◽  
Label Free Detection

The study describes development of a glycan biosensor for detection of a tumor-associated antibody. The glycan biosensor is built on an electrochemically activated/oxidized graphene screen-printed electrode (GSPE). Oxygen functionalities were subsequently applied for covalent immobilization of human serum albumin (HSA) as a natural nanoscaffold for covalent immobilization of Thomsen-nouvelle (Tn) antigen (GalNAc-O-Ser/Thr) to be fully available for affinity interaction with its analyte—a tumor-associated antibody. The step by step building process of glycan biosensor development was comprehensively characterized using a battery of techniques (scanning electron microscopy, atomic force microscopy, contact angle measurements, secondary ion mass spectrometry, surface plasmon resonance, Raman and energy-dispersive X-ray spectroscopy). Results suggest that electrochemical oxidation of graphene SPE preferentially oxidizes only the surface of graphene flakes within the graphene SPE. Optimization studies revealed the following optimal parameters: activation potential of +1.5 V vs. Ag/AgCl/3 M KCl, activation time of 60 s and concentration of HSA of 0.1 g L−1. Finally, the glycan biosensor was built up able to selectively and sensitively detect its analyte down to low aM concentration. The binding preference of the glycan biosensor was in an agreement with independent surface plasmon resonance analysis.

Simultaneous optical and electrochemical label-free biosensing with ITO-coated lossy-mode resonance sensor

2020 ◽  
Vol 154 ◽  
pp. 112050 ◽  
Author(s):  
Mateusz Śmietana ◽  
Marcin Koba ◽  
Petr Sezemsky ◽  
Katarzyna Szot-Karpińska ◽  
Dariusz Burnat ◽  
...  
Keyword(s):  
Label Free ◽  
Resonance Sensor ◽  
Electrochemical Label

Distinguishing cytosine methylation using electrochemical, label-free detection of DNA hybridization and ds-targets

2015 ◽  
Vol 64 ◽  
pp. 74-80 ◽  
Author(s):  
Bicheng Zhu ◽  
Marsilea A. Booth ◽  
Phillip Shepherd ◽  
Allan Sheppard ◽  
Jadranka Travas-Sejdic
Keyword(s):  
Dna Hybridization ◽  
Cytosine Methylation ◽  
Label Free ◽  
Electrochemical Label ◽  
Label Free Detection

scholarly journals Surface state of the dopamine RNA aptamer affects specific recognition and binding of dopamine by the aptamer-modified electrodes

The Analyst ◽  
2015 ◽  
Vol 140 (12) ◽  
pp. 4089-4096 ◽  
Author(s):  
Isabel Álvarez-Martos ◽  
Rui Campos ◽  
Elena E. Ferapontova
Keyword(s):  
Surface State ◽  
Surface Coverage ◽  
Modified Electrodes ◽  
Rna Aptamer ◽  
Label Free ◽  
Specific Recognition ◽  
Electrochemical Label

An electrochemical label-free aptasensor monitors physiological levels of dopamine with improved sensitivity when the aptamer surface coverage is optimized and with no interference from other catecholamines.

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