Direct electron transfer of PQQ-glucose dehydrogenase at modified carbon nanotubes electrodes

2011 ◽  
Vol 13 (11) ◽  
pp. 1240-1243 ◽  
Author(s):  
G. Göbel ◽  
I.W. Schubart ◽  
V. Scherbahn ◽  
F. Lisdat
Keyword(s):  
Carbon Nanotubes ◽  
Electron Transfer ◽  
Direct Electron Transfer ◽  
Glucose Dehydrogenase ◽  
Pqq Glucose Dehydrogenase ◽  
Modified Carbon Nanotubes

Efficient Direct Electron Transfer of PQQ-glucose Dehydrogenase on Carbon Cryogel Electrodes at Neutral pH

2011 ◽  
Vol 83 (14) ◽  
pp. 5721-5727 ◽  
Author(s):  
Victoria Flexer ◽  
Fabien Durand ◽  
Seiya Tsujimura ◽  
Nicolas Mano
Keyword(s):  
Electron Transfer ◽  
Direct Electron Transfer ◽  
Glucose Dehydrogenase ◽  
Neutral Ph ◽  
Pqq Glucose Dehydrogenase ◽  
Carbon Cryogel

Designer fungus FAD glucose dehydrogenase capable of direct electron transfer

2019 ◽  
Vol 123 ◽  
pp. 114-123 ◽  
Author(s):  
Kohei Ito ◽  
Junko Okuda-Shimazaki ◽  
Kazushige Mori ◽  
Katsuhiro Kojima ◽  
Wakako Tsugawa ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Direct Electron Transfer ◽  
Glucose Dehydrogenase

In Vitro Evaluation of Miniaturized Amperometric Enzyme Sensor Based on the Direct Electron Transfer Principle for Continuous Glucose Monitoring

2022 ◽  
pp. 193229682110706
Author(s):  
Yutaro Inoue ◽  
Yasuhide Kusaka ◽  
Kotaro Shinozaki ◽  
Inyoung Lee ◽  
Koji Sode
Keyword(s):  
Electron Transfer ◽  
Continuous Glucose Monitoring ◽  
Glucose Sensor ◽  
Direct Electron Transfer ◽  
Glucose Dehydrogenase ◽  
Glucose Monitoring ◽  
Reference Electrode ◽  
Enzyme Sensor

Background: The bacterial derived flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenase (FADGDH) is the most promising enzyme for the third-generation principle-based enzyme sensor for continuous glucose monitoring (CGM). Due to the ability of the enzyme to transfer electrons directly to the electrode, recognized as direct electron transfer (DET)-type FADGDH, although no investigation has been reported about DET-type FADGDH employed on a miniaturized integrated electrode. Methods: The miniaturized integrated electrode was formed by sputtering gold (Au) onto a flexible film with 0.1 mm in thickness and divided into 3 parts. After an insulation layer was laminated, 3 openings for a working electrode, a counter electrode and a reference electrode were formed by dry etching. A reagent mix containing 1.2 × 10−4 Unit of DET-type FADGDH and carbon particles was deposited. The long-term stability of sensor was evaluated by continuous operation, and its performance was also evaluated in the presence of acetaminophen and the change in oxygen partial pressure (pO2) level. Results: The amperometric response of the sensor showed a linear response to glucose concentration up to 500 mg/dL without significant change of the response over an 11-day continuous measurement. Moreover, the effect of acetaminophen and pO2 on the response were negligible. Conclusions: These results indicate the superb potential of the DET-type FADGDH-based sensor with the combination of a miniaturized integrated electrode. Thus, the described miniaturized DET-type glucose sensor for CGM will be a promising tool for effective glycemic control. This will be further investigated using an in vivo study.

Third generation impedimetric sensor employing direct electron transfer type glucose dehydrogenase

2019 ◽  
Vol 129 ◽  
pp. 189-197 ◽  
Author(s):  
Yuka Ito ◽  
Junko Okuda-Shimazaki ◽  
Wakako Tsugawa ◽  
Noya Loew ◽  
Isao Shitanda ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Direct Electron Transfer ◽  
Glucose Dehydrogenase ◽  
Third Generation
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