Scanning electrochemical microscopy of single human urinary bladder cells using reactive oxygen species as probe of inflammatory response

2010 ◽  
Vol 12 (6) ◽  
pp. 773-776 ◽  
Author(s):  
Xiaocui Zhao ◽  
Simon Lam ◽  
Jana Jass ◽  
Zhifeng Ding
Keyword(s):  
Reactive Oxygen Species ◽  
Urinary Bladder ◽  
Inflammatory Response ◽  
Reactive Oxygen ◽  
Scanning Electrochemical Microscopy ◽  
Oxygen Species ◽  
Human Urinary Bladder ◽  
Bladder Cells ◽  
Electrochemical Microscopy

Redox reactions of reactive oxygen species in aqueous solutions as the probe for scanning electrochemical microscopy of single live T24 cells

2010 ◽  
Vol 88 (6) ◽  
pp. 569-576 ◽  
Author(s):  
Xiaocui Zhao ◽  
Mengni Zhang ◽  
Yitao Long ◽  
Zhifeng Ding
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Aqueous Solutions ◽  
Redox Reactions ◽  
Reactive Oxygen ◽  
Scanning Electrochemical Microscopy ◽  
Electrochemical Technique ◽  
Oxygen Species ◽  
T24 Cells ◽  
Electrochemical Microscopy

The redox reactions of two main components of reactive oxygen species (ROS), superoxide and hydrogen peroxide, along with oxygen in aqueous solutions were investigated using a conventional electrochemical technique, differential pulse voltammetry (DPV). Superoxide undergoes oxidation at a Pt working electrode biased at 0.055 V versus Ag/AgCl, while hydrogen peroxide can be oxidized and reduced at 0.817 and –0.745 V, respectively. Oxygen in the solutions is reduced at the electrode with an applied potential of –0.455 V. Based on these results, hydrogen peroxide and superoxide released from live cells can be successfully monitored, identified, and mapped using scanning electrochemical microscopy (SECM) at different potentials. Single human bladder (T24) cells were imaged using a 5 μm diameter SECM probe biased at –0.400, –0.600, and –0.800 V. Oxygen reduction that seems an interference can be discriminated from that of hydrogen peroxide by means of SECM.

Scanning Electrochemical Microscopy Studies of Glutathione-Modified Surfaces. An Erasable and Sensitive-to-Reactive Oxygen Species Surface

Langmuir ◽  
2011 ◽  
Vol 27 (17) ◽  
pp. 11206-11211 ◽  
Author(s):  
Alina Latus ◽  
Jean-Marc Noël ◽  
Elena Volanschi ◽  
Corinne Lagrost ◽  
Philippe Hapiot
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Scanning Electrochemical Microscopy ◽  
Oxygen Species ◽  
Modified Surfaces ◽  
Electrochemical Microscopy

scholarly journals Reactive oxygen species formed in organic lithium–oxygen batteries

2016 ◽  
Vol 18 (16) ◽  
pp. 10774-10780 ◽  
Author(s):  
Patrick Schwager ◽  
Saustin Dongmo ◽  
Daniela Fenske ◽  
Gunther Wittstock
Keyword(s):  
Reactive Oxygen Species ◽  
Fluorescence Microscopy ◽  
Reactive Oxygen ◽  
Scanning Electrochemical Microscopy ◽  
Oxygen Species ◽  
Organic Electrolytes ◽  
Lithium Oxygen Batteries ◽  
Electrochemical Microscopy ◽  
Discharge Reaction

The generation of reactive oxygen species has been assumed to occur during the charging reaction of lithium-oxygen batteries with organic electrolytes. Here we show independently by fluorescence microscopy and scanning electrochemical microscopy that superoxide is also formed and released into the solution during the discharge reaction.

Co-regulation of Cxcl1 and versican in inflammatory response in UVB induced reactive oxygen species in the skin

2017 ◽  
Vol 86 (2) ◽  
pp. e80
Author(s):  
Chihiro Takemori ◽  
Makoto Kunisada ◽  
Flandiana Yogianti ◽  
Sugako Oka ◽  
Kunihiko Sakumi ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Inflammatory Response ◽  
Reactive Oxygen ◽  
Oxygen Species
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