A self-assembled monolayer based electrochemical immunosensor for detection of leukemia K562A cells

2007 ◽  
Vol 9 (6) ◽  
pp. 1359-1364 ◽  
Author(s):  
Chen Hao ◽  
Feng Yan ◽  
Lin Ding ◽  
Yadong Xue ◽  
Huangxian Ju
Keyword(s):  
Electrochemical Immunosensor ◽  
Self Assembled Monolayer ◽  
Self Assembled

scholarly journals Impedimetric and Plasmonic Sensing of Collagen I Using a Half-Antibody-Supported, Au-Modified, Self-Assembled Monolayer System

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 227
Author(s):  
Marcin Gwiazda ◽  
Sheetal K. Bhardwaj ◽  
Ewa Kijeńska-Gawrońska ◽  
Wojciech Swieszkowski ◽  
Unni Sivasankaran ◽  
...  
Keyword(s):  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Covalent Immobilization ◽  
Electrochemical Immunosensor ◽  
Collagen I ◽  
Enzyme Linked Immunosorbent Assay ◽  
Type I ◽  
Self Assembled Monolayer ◽  
Self Assembled

This research presents an electrochemical immunosensor for collagen I detection using a self-assembled monolayer (SAM) of gold nanoparticles (AuNPs) and covalently immobilized half-reduced monoclonal antibody as a receptor; this allowed for the validation of the collagen I concentration through two different independent methods: electrochemically by Electrochemical Impedance Spectroscopy (EIS), and optically by Surface Plasmon Resonance (SPR). The high unique advantage of the proposed sensor is based on the performance of the stable covalent immobilization of the AuNPs and enzymatically reduced half-IgG collagen I antibodies, which ensured their appropriate orientation onto the sensor’s surface, good stability, and sensitivity properties. The detection of collagen type I was performed in a concentration range from 1 to 5 pg/mL. Moreover, SPR was utilized to confirm the immobilization of the monoclonal half-antibodies and sensing of collagen I versus time. Furthermore, EIS experiments revealed a limit of detection (LOD) of 0.38 pg/mL. The selectivity of the performed immunosensor was confirmed by negligible responses for BSA. The performed approach of the immunosensor is a novel, innovative attempt that enables the detection of collagen I with very high sensitivity in the range of pg/mL, which is significantly lower than the commonly used enzyme-linked immunosorbent assay (ELISA).

A label-free electrochemical immunosensor for beta-amyloid detection

2016 ◽  
Vol 8 (31) ◽  
pp. 6115-6120 ◽  
Author(s):  
Ajeet Kaushik ◽  
Pratikkumar Shah ◽  
Phani Kiran Vabbina ◽  
Rahul Dev Jayant ◽  
Sneham Tiwari ◽  
...  
Keyword(s):  
Beta Amyloid ◽  
Electrochemical Immunosensor ◽  
Label Free ◽  
Self Assembled Monolayer ◽  
Interdigitated Electrode ◽  
Label Free Detection ◽  
Self Assembled

A label-free detection of beta-amyloid (βA) proteins using an electrochemical immunosensor fabricated via immobilizing specific anti-beta-amyloid antibodies (An-βA-Abs) onto an interdigitated electrode of gold (IDE-Au) modified using a self-assembled monolayer (SAM) of dithiobis(succinimidyl propionate) [DTSP] is presented here.

Self-Assembled Fluid Phase Floating Membranes with Tuneable Water Interlayers

2019 ◽  
Author(s):  
Luke Clifton ◽  
Nicoló Paracini ◽  
Arwel V. Hughes ◽  
Jeremy H. Lakey ◽  
Nina-Juliane Seinke ◽  
...  
Keyword(s):  
Fluid Phase ◽  
Interlayer Thickness ◽  
Supported Bilayers ◽  
Self Assembled Monolayer ◽  
High Coverage ◽  
Nano Technology ◽  
Surface Distance ◽  
Potential Applications ◽  
Self Assembled ◽  
Coated Surfaces

<p>We present a reliable method for the fabrication of fluid phase unsaturated bilayers which are readily self-assembled on charged self-assembled monolayer (SAM) surfaces producing high coverage floating supported bilayers where the membrane to surface distance could be controlled with nanometer precision. Vesicle fusion was used to deposit the bilayers onto anionic SAM coated surfaces. Upon assembly the bilayer to SAM solution interlayer thickness was 7-10 Å with evidence suggesting that this layer was present due to SAM hydration repulsion of the bilayer from the surface. This distance could be increased using low concentrations of salts which caused the interlayer thickness to enlarge to ~33 Å. Reducing the salt concentration resulted in a return to a shorter bilayer to surface distance. These accessible and controllable membrane models are well suited to a range of potential applications in biophysical studies, bio-sensors and Nano-technology.</p><br>

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