Site specific/targeted delivery of gemcitabine through anisamide anchored chitosan/poly ethylene glycol nanoparticles: An improved understanding of lung cancer therapeutic intervention

2012 ◽  
Vol 47 (5) ◽  
pp. 1006-1014 ◽  
Author(s):  
Neeraj K. Garg ◽  
Priya Dwivedi ◽  
Christopher Campbell ◽  
Rajeev K. Tyagi
Keyword(s):  
Lung Cancer ◽  
Ethylene Glycol ◽  
Therapeutic Intervention ◽  
Targeted Delivery ◽  
Poly Ethylene Glycol ◽  
Site Specific ◽  
Poly Ethylene

scholarly journals The Importance of Poly(ethylene glycol) and Lipid Structure in Targeted Gene Delivery to Lymph Nodes by Lipid Nanoparticles

Pharmaceutics ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1068 ◽  
Author(s):  
Danijela Zukancic ◽  
Estelle J. A. Suys ◽  
Emily H. Pilkington ◽  
Azizah Algarni ◽  
Hareth Al-Wassiti ◽  
...  
Keyword(s):  
Lymph Node ◽  
Gene Delivery ◽  
Ethylene Glycol ◽  
Lymph Nodes ◽  
Injection Site ◽  
Targeted Delivery ◽  
Lipid Nanoparticles ◽  
Tween 20 ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Targeted delivery of nucleic acids to lymph nodes is critical for the development of effective vaccines and immunotherapies. However, it remains challenging to achieve selective lymph node delivery. Current gene delivery systems target mainly to the liver and typically exhibit off-target transfection at various tissues. Here we report novel lipid nanoparticles (LNPs) that can deliver plasmid DNA (pDNA) to a draining lymph node, thereby significantly enhancing transfection at this target organ, and substantially reducing gene expression at the intramuscular injection site (muscle). In particular, we discovered that LNPs stabilized by 3% Tween 20, a surfactant with a branched poly(ethylene glycol) (PEG) chain linking to a short lipid tail, achieved highly specific transfection at the lymph node. This was in contrast to conventional LNPs stabilized with a linear PEG chain and two saturated lipid tails (PEG-DSPE) that predominately transfected at the injection site (muscle). Interestingly, replacing Tween 20 with Tween 80, which has a longer unsaturated lipid tail, led to a much lower transfection efficiency. Our work demonstrates the importance of PEGylation in selective organ targeting of nanoparticles, provides new insights into the structure–property relationship of LNPs, and offers a novel, simple, and practical PEGylation technology to prepare the next generation of safe and effective vaccines against viruses or tumours.

Site-Specific Cross-Linking of Galectin-1 Homodimers via Poly(ethylene glycol) Bismaleimide

2021 ◽  
Author(s):  
Bryant J. Kane ◽  
Margaret M. Fettis ◽  
Shaheen A. Farhadi ◽  
Renjie Liu ◽  
Gregory A. Hudalla
Keyword(s):  
Ethylene Glycol ◽  
Cross Linking ◽  
Poly Ethylene Glycol ◽  
Site Specific ◽  
Poly Ethylene
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