Porous microspheres as promising vehicles for the topical delivery of poorly soluble asiaticoside accelerate wound healing and inhibit scar formation in vitro & in vivo

2016 ◽  
Vol 109 ◽  
pp. 1-13 ◽  
Author(s):  
Chen-Zhen Zhang ◽  
Jie Niu ◽  
Yee-Song Chong ◽  
Yan-Fen Huang ◽  
Yang Chu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Topical Delivery ◽  
Scar Formation ◽  
Porous Microspheres ◽  
Accelerate Wound Healing ◽  
Poorly Soluble

scholarly journals Resveratrol-loaded peptide-hydrogels inhibit scar formation in wound healing through suppressing inflammation

2019 ◽  
Author(s):  
Chen-Chen Zhao ◽  
Lian Zhu ◽  
Zheng Wu ◽  
Rui Yang ◽  
Na Xu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Damage Model ◽  
Skin Wound ◽  
Scar Formation ◽  
Wound Dressings ◽  
Skin Damage ◽  
Skin Wound Healing ◽  
Accelerate Wound Healing ◽  
Peptide Hydrogels

Abstract Scar formation seriously affects the repair of damaged skin especially in adults and the excessive inflammation has been considered as the reason. The self-assembled peptide-hydrogels are ideal biomaterials for skin wound healing due to their similar nanostructure to natural extracellular matrix, hydration environment and serving as drug delivery systems. In our study, resveratrol, a polyphenol compound with anti-inflammatory effect, is loaded into peptide-hydrogel (Fmoc-FFGGRGD) to form a wound dressing (Pep/RES). Resveratrol is slowly released from the hydrogel in situ, and the release amount is controlled by the loading amount. The in vitro cell experiments demonstrate that the Pep/RES has no cytotoxicity and can inhibit the production of pro-inflammatory cytokines of macrophages. The Pep/RES hydrogels are used as wound dressings in rat skin damage model. The results suggest that the Pep/RES dressing can accelerate wound healing rate, exhibit well-organized collagen deposition, reduce inflammation and eventually prevent scar formation. The Pep/RES hydrogels supply a potential product to develop new skin wound dressings for the therapy of skin damage.

scholarly journals Bioinspired mechanically active adhesive dressings to accelerate wound closure

2019 ◽  
Vol 5 (7) ◽  
pp. eaaw3963 ◽  
Author(s):  
S. O. Blacklow ◽  
J. Li ◽  
B. R. Freedman ◽  
M. Zeidi ◽  
C. Chen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Closure ◽  
Healing Process ◽  
Skin Wound ◽  
Wound Management ◽  
In Vivo Studies ◽  
Skin Wound Healing ◽  
Accelerate Wound Healing

Inspired by embryonic wound closure, we present mechanically active dressings to accelerate wound healing. Conventional dressings passively aid healing by maintaining moisture at wound sites. Recent developments have focused on drug and cell delivery to drive a healing process, but these methods are often complicated by drug side effects, sophisticated fabrication, and high cost. Here, we present novel active adhesive dressings consisting of thermoresponsive tough adhesive hydrogels that combine high stretchability, toughness, tissue adhesion, and antimicrobial function. They adhere strongly to the skin and actively contract wounds, in response to exposure to the skin temperature. In vitro and in vivo studies demonstrate their efficacy in accelerating and supporting skin wound healing. Finite element models validate and refine the wound contraction process enabled by these active adhesive dressings. This mechanobiological approach opens new avenues for wound management and may find broad utility in applications ranging from regenerative medicine to soft robotics.

Green light emitting diodes accelerate wound healing: Characterization of the effect and its molecular basis in vitro and in vivo

2012 ◽  
Vol 20 (2) ◽  
pp. 226-235 ◽  
Author(s):  
Tomohiro Fushimi ◽  
Shigeki Inui ◽  
Takeshi Nakajima ◽  
Masahiro Ogasawara ◽  
Ko Hosokawa ◽  
...  
Keyword(s):  
Wound Healing ◽  
Molecular Basis ◽  
Light Emitting Diodes ◽  
Green Light ◽  
Light Emitting ◽  
Accelerate Wound Healing

scholarly journals Saliva Exosomes-Derived UBE2O Promotes Angiogenesis in Cutaneous Wounds by Targeting SMAD6

2020 ◽  
Author(s):  
Guohui Liu ◽  
Bobin Mi ◽  
Lang Chen ◽  
Yuan Xiong ◽  
Chenchen Yan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Cutaneous Wounds ◽  
Accelerate Wound Healing ◽  
Ubiquitin Conjugating Enzyme ◽  
Novel Strategy ◽  
Underlying Mechanisms ◽  
Main Components

Abstract Background Enhancing angiogenesis is critical for accelerating wound healing. Application of different types of exosomes (Exos) to promote angiogenesis represents a novel strategy for enhanced wound repair. Saliva is known to accelerate wound healing, but the underlying mechanisms remain unclear. Results Our results have demonstrated that saliva-derived exosomes (saliva-Exos) induce HUVEC proliferation, migration, and angiogenesis in vitro, and promote cutaneous wound healing in vivo. Further experiments documented that Ubiquitin-conjugating enzyme E2O (UBE2O) is one of the main components of saliva-Exos, and activation of UBE2O has effects similar to those of saliva-Exos, both in vitro and in vivo. Mechanistically, UBE2O decreases the level of SMAD6, thereby activating BMP2, which, in turn, induces angiogenesis. Conclusions The present work suggests that administration of saliva-Exos and UBE2O represents a promising strategy for enhancing wound healing through promotion of angiogenesis.

scholarly journals Sulfated Polysaccharides From Sea Algae as the Basis of Modern Biotechnologies for Creating Wound Coverings: Current Achievements and Coming Prospects

2020 ◽  
Author(s):  
Boris Andryukov ◽  
Natalya Besednova ◽  
Tatyana Kuznetsova ◽  
Tatyana Zaporozhets ◽  
Svetlana Ermakova ◽  
...  
Keyword(s):  
Wound Healing ◽  
Clinical Trial Data ◽  
Biological Properties ◽  
Biologically Active ◽  
Wound Dressings ◽  
Sulfated Polysaccharides ◽  
Accelerate Wound Healing ◽  
In Vivo Experiments

Wound healing involves a complex cascade of cellular, molecular, and biochemical responses and signaling processes. It consists of successive interrelated phases, the duration of which depends on multifactorial processes. Wound treatment is a major healthcare issue that can be resolved by development of effective and affordable wound dressings based on natural materials and biologically active substances. Proper use of modern wound dressings can significantly accelerate wound healing with minimal cosmetic defects. The innovative biotechnologies for creating modern natural interactive dressings are based on sulfated polysaccharides from seaweeds with their unique structures and biological properties, the availability of their sources in the form of wild bushes, and in the form of aquaculture, as well as with a high potential for participation in process control wound healing. These natural biopolymers are a novel and promising biologically active source for designing wound dressings based on alginates, fucoidans, carrageenans, and ulvans, which serve as active and effective therapeutic tools. The aim of this review is to summarize available information about the modern wound dressing’s technologies based on seaweed-derived polysaccharides, including those successfully implemented in commercial products, with the emphasis on promising and innovative designs. The further prospect of using marine biopolymers is related to the need to analyze the results of numerous in vitro and in vivo experiments, summarize clinical trial data, develop a scientifically based approach and relevant practical recommendations for the treatment of wounds.

Palmatine-loaded electrospun poly(ε-caprolactone)/gelatin nanofibrous scaffolds accelerate wound healing and inhibit hypertrophic scar formation in a rabbit ear model

2020 ◽  
pp. 088532822095006
Author(s):  
Zhimin Jiang ◽  
Lichi Zhao ◽  
Feixiang He ◽  
Haixin Tan ◽  
Yongling Li ◽  
...  
Keyword(s):  
Wound Healing ◽  
Hypertrophic Scar ◽  
Antioxidant Activities ◽  
Psychological Trauma ◽  
Medical Problem ◽  
Control Group ◽  
Accelerate Wound Healing ◽  
Nanofibrous Scaffolds

Hypertrophic scar (HS) has been considered as a great concern for patients and a challenging problem for clinicians as it can cause functional debility, cosmetic disfigurement and psychological trauma. Although many methods have been developed to prevent and treat HS, the scarless healing is still a worldwide medical problem. In this study, palmatine-loaded poly( ε-caprolactone)/gelatin nanofibrous scaffolds (PCL/GE/PALs) were fabricated by electrospinning, and their effects on wound healing and HS formation were investigated. These nanofiber mats exhibit good antibacterial and antioxidant activities. In vitro studies indicate PCL/GE/PAL scaffolds can facilitate the adhesion, spreading and proliferation of L929 fibroblasts. In vivo tests demonstrate the full-thickness wounds treated with PCL/GE/PAL scaffolds heal about 3.5 days earlier than those in the control group. Scar elevation index measurements and histological analyses reveal PCL/GE/PAL scaffolds significantly inhibit HS formation, with the decrease in the thickness of dermis and epidermis, the number of fibroblasts, as well as the density of collagen and microvascular. Accelerating wound healing and inhibiting HS formation of these scaffolds are contributed to the sustained release of palmatine. The present work validates the potential use of palmatine-loaded electrospun nanofibrous scaffold PCL/GE/PALs as a functional wound dressing for healing wounds and preventing HS formation.

Fabrication of a novel blended membrane with chitosan and silk microfibers for wound healing: characterization, in vitro and in vivo studies

2015 ◽  
Vol 3 (17) ◽  
pp. 3634-3642 ◽  
Author(s):  
Zongpu Xu ◽  
Liyang Shi ◽  
Mingying Yang ◽  
Haiping Zhang ◽  
Liangjun Zhu
Keyword(s):  
Wound Healing ◽  
In Vivo Studies ◽  
Accelerate Wound Healing ◽  
Healing Efficiency ◽  
Blended Membrane

A novel type of chitosan/silk microfibers blended membrane was fabricated, which could significantly accelerate wound healing efficiency.

scholarly journals Effects of cardiotonic steroids on dermal collagen synthesis and wound healing

2008 ◽  
Vol 105 (1) ◽  
pp. 30-36 ◽  
Author(s):  
Nasser El-Okdi ◽  
Sleiman Smaili ◽  
Vanamala Raju ◽  
Amjad Shidyak ◽  
Shalini Gupta ◽  
...  
Keyword(s):  
Wound Healing ◽  
Collagen Synthesis ◽  
Cardiac Fibroblasts ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Accelerate Wound Healing ◽  
Proline Incorporation ◽  
Dermal Collagen

We previously reported that cardiotonic steroids stimulate collagen synthesis by cardiac fibroblasts in a process that involves signaling through the Na-K-ATPase pathway (Elkareh et al. Hypertension 49: 215–224, 2007). In this study, we examined the effect of cardiotonic steroids on dermal fibroblasts collagen synthesis and on wound healing. Increased collagen expression by human dermal fibroblasts was noted in response to the cardiotonic steroid marinobufagenin in a dose- and time-dependent fashion. An eightfold increase in collagen synthesis was noted when cells were exposed to 10 nM marinobufagenin for 24 h ( P < 0.01). Similar increases in proline incorporation were seen following treatment with digoxin, ouabain, and marinobufagenin (10 nM × 24 h, all results P < 0.01 vs. control). The coadministration of the Src inhibitor PP2 or N-acetylcysteine completely prevented collagen stimulation by marinobufagenin. Next, we examined the effect of digoxin, ouabain, and marinobufagenin on the rate of wound closure in an in vitro model where human dermal fibroblasts cultures were wounded with a pipette tip and monitored by digital microscopy. Finally, we administered digoxin in an in vivo wound healing model. Olive oil was chosen as the digoxin carrier because of a favorable partition coefficient observed for labeled digoxin with saline. This application significantly accelerated in vivo wound healing in rats wounded with an 8-mm biopsy cut. Increased collagen accumulation was noted 9 days after wounding (both P < 0.01). The data suggest that cardiotonic steroids induce increases in collagen synthesis by dermal fibroblasts, as could potentially be exploited to accelerate wound healing.

Solid Lipid Nanoparticles (SLNs) Gels for Topical Delivery of Aceclofenac in vitro and in vivo Evaluation

2013 ◽  
Vol 10 (6) ◽  
pp. 656-666 ◽  
Author(s):  
Sandipan Dasgupta ◽  
Surajit Ghosh ◽  
Subhabrata Ray ◽  
Bhaskar Mazumder
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Topical Delivery ◽  
Lipid Nanoparticles ◽  
In Vivo Evaluation ◽  
Solid Lipid
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