Intratumour microbiome associated with the infiltration of cytotoxic CD8+ T cells and patient survival in cutaneous melanoma

2021 ◽  
Vol 151 ◽  
pp. 25-34
Author(s):  
Gongjian Zhu ◽  
Haixiang Su ◽  
Caroline H. Johnson ◽  
Sajid A. Khan ◽  
Harriet Kluger ◽  
...  
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Cutaneous Melanoma ◽  
Patient Survival

scholarly journals Relationship between PD-L1 expression, CD8+ T-cell infiltration and prognosis in intrahepatic cholangiocarcinoma patients

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Min Deng ◽  
Shao-Hua Li ◽  
Xu Fu ◽  
Xiao-Peng Yan ◽  
Jun Chen ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Cd8 T Cells ◽  
Intrahepatic Cholangiocarcinoma ◽  
Patient Survival ◽  
Disease Free Survival ◽  
Cd8 T Cell ◽  
Future Research ◽  
Programmed Death ◽  
Expression Rate

Abstract Background Programmed death- ligand 1 (PD-L1) seems to be associated with the immune escape of tumors, and immunotherapy may be a favorable treatment for PD-L1-positive patients. We evaluated intrahepatic cholangiocarcinoma (ICC) specimens for their expression of PD-L1, infiltration of CD8+ T cells, and the relationship between these factors and patient survival. Methods In total, 69 resections of ICC were stained by immunohistochemistry for PD-L1, programmed death factor-1 (PD-1), and CD8+ T cells. CD8+ T-cell densities were analyzed both within tumors and at the tumor-stromal interface. Patient survival was predicted based on the PD-L1 status and CD8+ T-cell density. Results The expression rate of PD-L1 was 12% in cancer cells and 51% in interstitial cells. The expression rate of PD-1 was 30%, and the number of CD8+ T-cells increased with the increase of PD-L1 expression (p < 0.05). The expression of PD-L1 in the tumor was correlated with poor overall survival(OS) (p = 0.004), and the number of tumor and interstitial CD8+ T-cells was correlated with poor OS and disease-free survival (DFS) (All p < 0.001). Conclusions The expression of PD-L1 in the tumor is related to poor OS, and the number of tumor or interstitial CD8+ T-cells is related to poor OS and DFS. For patients who lose their chance of surgery, PD-L1 immunosuppressive therapy may be the focus of future research as a potential treatment.

scholarly journals Vaccination-induced skin-resident memory CD8+T cells mediate strong protection against cutaneous melanoma

2018 ◽  
Vol 7 (7) ◽  
pp. e1442163 ◽  
Author(s):  
Felipe Gálvez-Cancino ◽  
Ernesto López ◽  
Evelyn Menares ◽  
Ximena Díaz ◽  
Camila Flores ◽  
...  
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Cutaneous Melanoma ◽  
Memory Cd8 T Cells

Identification of immune-related biomarkers associated with tumorigenesis and prognosis in cutaneous melanoma patients

2020 ◽  
Author(s):  
Biao Huang ◽  
Wei Han ◽  
Zu-Feng Sheng ◽  
Guo-Liang Shen
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Cd8 T Cells ◽  
Cutaneous Melanoma ◽  
Normal Skin ◽  
Extensive Study ◽  
Differentially Expressed ◽  
Hub Genes ◽  
Bioinformatics Analyses ◽  
Normal Tissues

Abstract Background：Skin cutaneous melanoma (SKCM) is one of the most malignant and aggressive cancers, causing about 72% of deaths in skin carcinoma. Although extensive study has explored the mechanism of recurrence and metastasis, the tumorigenesis of cutaneous melanoma remains unclear. Exploring the tumorigenesis mechanism may help identify prognostic biomarkers that could serve to guide cancer therapy. Methods：Integrative bioinformatics analyses, including GEO database, TCGA database, DAVID, STRING, Metascape, GEPIA, cBioPortal, TRRUST, TIMER, TISIDB and DGIdb, were performed to unveil the hub genes participating in tumor progression and cancer-associated immunology of SKCM. Furthermore, immunohistochemistry (IHC) staining was performed to validate differential expression levels of hub genes between SKCM tissue and normal tissues from the First Affiliated Hospital of Soochow University cohort. Results: A total of 308 differentially expressed genes (DEGs) and 12 hub genes were found significantly differentially expressed between SKCM and normal skin tissues. Functional annotation indicated that inflammatory response, immune response was closely associated with SKCM tumorigenesis. KEGG pathways in hub genes include IL-10 signaling and chemokine receptors bind chemokine signaling. Five chemokines members (CXCL9, CXCL10, CXCL13, CCL4, CCL5) were associated with better overall survival and pathological stages. IHC results suggested that significantly elevated CXCL9, CXCL10, CXCL13, CCL4 and CCL5 proteins expressed in the SKCM than in the normal tissues. Moreover, our findings suggested that IRF7, RELA, NFKB1, IRF3 and IRF1 are key transcription factors for CCL4, CCL5, CXCL10. In addition, the expressions of CXCL9, CXCL10, CXCL13, CCL4 and CCL5 were positively correlated with infiltration of six immune cells (B-cell, CD8+T cells, CD4+T cells, macrophages, neutrophils, dendritic cells) and 28 types of TILs. Among them, high levels of B cells, CD8+T cells, neutrophils and dendritic cells were significantly related to longer SKCM survival time. Conclusion: In summary, this study mainly identified five chemokine members (CXCL9, CXCL10, CXCL13, CCL4, CCL5) associated with SKCM tumorigenesis, progression, prognosis and immune infiltrations, which might help us evaluate several immune-related targets for cutaneous melanoma therapy.

Relationship Between PD-L1 Expression, CD8+ T-Cell Infiltration and Prognosis in Intrahepatic Cholangiocarcinoma Patients

2021 ◽  
Author(s):  
Min Deng ◽  
Shao-Hua Li ◽  
Xu Fu ◽  
Xiao-Peng Yan ◽  
Jun Chen ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Cd8 T Cells ◽  
Immune Escape ◽  
Patient Survival ◽  
Disease Free Survival ◽  
Cd8 T Cell ◽  
Future Research ◽  
Programmed Death ◽  
Expression Rate

Abstract Background: Programmed death- ligand 1(PD-L1) seems to be associated with the immune escape of tumors, and immunotherapy may be a favorable treatment for PD-L1-positive patients. We evaluated ICC specimens for their expression of PD-L1, infiltration of CD8+ T cells, and the relationship between these factors and patient survival.Methods: In total, 69 resections of ICC were stained by immunohistochemistry for PD-L1, programmed death factor-1(PD-1), and CD8+ T cells. CD8+ T-cell densities were analyzed both within tumors and at the tumor-stromal interface. Patient survival was predicted based on the PD-L1 status and CD8+ T-cell density.Results: The expression rate of PD-L1 was 12% in cancer cells and 51% in interstitial cells. The expression rate of PD-1 was 30%, and the number of CD8+ T-cells increased with the increase of PD-L1 expression (p<0.05). The expression of PD-L1 in the tumor was correlated with poor overall survival(OS) (p=0.004), and the number of tumor and interstitial CD8+ T-cells was correlated with poor OS and disease-free survival (DFS) (All p<0.001).Conclusions: The expression of PD-L1 in the tumor is related to poor OS, and the number of tumor or interstitial CD8+ T-cells is related to poor OS and DFS. For patients who lose their chance of surgery, PD-L1 immunosuppressive therapy may be the focus of future research as a potential treatment.

scholarly journals Accumulation of CD103 + CD8 + T cells in a cutaneous melanoma micrometastasis

2019 ◽  
Vol 8 (12) ◽  
Author(s):  
Katharina Hochheiser ◽  
Han Xian Aw Yeang ◽  
Teagan Wagner ◽  
Candani Tutuka ◽  
Andreas Behren ◽  
...  
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Cutaneous Melanoma

scholarly journals Abstract 3967: High microsomal PGE2synthase-1 levels associate with low CD8 T cells and poorer melanoma patient survival

2017 ◽  
Author(s):  
Sun-Hee Kim ◽  
Jason Roszik ◽  
Weiyi Peng ◽  
Suhendan Ekmekcioglu ◽  
Elizabeth A. Grimm
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Melanoma Patient ◽  
Patient Survival

scholarly journals Tumor-infiltrating, interleukin-33-producing effector-memory CD8+T cells in resected hepatocellular carcinoma prolong patient survival

Hepatology ◽  
2015 ◽  
Vol 61 (6) ◽  
pp. 1957-1967 ◽  
Author(s):  
Stefan M. Brunner ◽  
Christoph Rubner ◽  
Rebecca Kesselring ◽  
Maria Martin ◽  
Eva Griesshammer ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
T Cells ◽  
Cd8 T Cells ◽  
Patient Survival ◽  
Effector Memory ◽  
Memory Cd8 T Cells ◽  
Interleukin 33

Author response for "4‐1BB costimulation promotes bystander activation of human CD8 T cells"

2020 ◽  
Author(s):  
Manuel Reithofer ◽  
Sandra Rosskopf ◽  
Judith Leitner ◽  
Claire Battin ◽  
Barbara Bohle ◽  
...  
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Author Response ◽  
Bystander Activation
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