scholarly journals Prognostic significance of an 11-gene RNA assay in archival tissue of cutaneous melanoma stage I–III patients

2021 ◽  
Vol 143 ◽  
pp. 11-18
Author(s):  
Thilo Gambichler ◽  
Konstantinos Tsagoudis ◽  
Felix Kiecker ◽  
Uwe Reinhold ◽  
Eggert Stockfleth ◽  
...  
Keyword(s):  
Cutaneous Melanoma ◽  
Prognostic Significance ◽  
Stage I ◽  
Archival Tissue

scholarly journals Prognostic Significance of DNA Aneuploidy in Stage I Cutaneous Melanoma

1988 ◽  
Vol 207 (4) ◽  
pp. 455-461 ◽  
Author(s):  
SONIA M. KHEIR ◽  
STEVEN D. BINES ◽  
JAMIE H. VONROENN ◽  
SENG-JAW SOONG ◽  
MARSHALL M. URIST ◽  
...  
Keyword(s):  
Cutaneous Melanoma ◽  
Prognostic Significance ◽  
Stage I ◽  
Dna Aneuploidy

scholarly journals CCL20/TNF/VEGFA Cytokine Secretory Phenotype of Tumor-Associated Macrophages Is a Negative Prognostic Factor in Cutaneous Melanoma

Cancers ◽  
2021 ◽  
Vol 13 (16) ◽  
pp. 3943
Author(s):  
Alba Gutiérrez-Seijo ◽  
Elena García-Martínez ◽  
Celia Barrio-Alonso ◽  
Miriam Pareja-Malagón ◽  
Alejandra Acosta-Ocampo ◽  
...  
Keyword(s):  
Cutaneous Melanoma ◽  
Large Fraction ◽  
Prognostic Significance ◽  
Disease Free Survival ◽  
Rna Seq ◽  
Primary Cutaneous Melanoma ◽  
Kaplan Meier ◽  
Intracellular Cytokines ◽  
Negative Prognostic Factor

TAMs constitute a large fraction of infiltrating immune cells in melanoma tissues, but their significance for clinical outcomes remains unclear. We explored diverse TAM parameters in clinically relevant primary cutaneous melanoma samples, including density, location, size, and polarization marker expression; in addition, because cytokine production is a hallmark of macrophages function, we measured CCL20, TNF, and VEGFA intracellular cytokines by single-cell multiparametric confocal microscopy. The Kaplan–Meier method was used to analyze correlation with melanoma-specific disease-free survival and overall survival. No significant correlations with clinical parameters were observed for TAM density, morphology, or location. Significantly, higher contents of the intracellular cytokines CCL20, TNF, and VEGFA were quantified in TAMs infiltrating metastasizing compared to non-metastasizing skin primary melanomas (p < 0.001). To mechanistically explore cytokine up-regulation, we performed in vitro studies with melanoma-conditioned macrophages, using RNA-seq to explore involved pathways and specific inhibitors. We show that p53 and NF-κB coregulate CCL20, TNF, and VEGFA in melanoma-conditioned macrophages. These results delineate a clinically relevant pro-oncogenic cytokine profile of TAMs with prognostic significance in primary melanomas and point to the combined therapeutic targeting of NF-kB/p53 pathways to control the deviation of TAMs in melanoma.

Immunocytochemical markers in stage I lung cancer: relevance to prognosis.

1997 ◽  
Vol 15 (8) ◽  
pp. 2858-2865 ◽  
Author(s):  
U Pastorino ◽  
S Andreola ◽  
E Tagliabue ◽  
F Pezzella ◽  
M Incarbone ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Multivariate Analysis ◽  
Cox Model ◽  
Statistical Significance ◽  
Prognostic Significance ◽  
Stage I ◽  
Laminin Receptor ◽  
Independent Predictive Factor ◽  
Time To Recurrence ◽  
Egfr Expression

PURPOSE This study investigated the frequency of the expression and prognostic significance of a panel of immunocytochemical markers in resected non-small-cell lung cancer (NSCLC). PATIENTS AND METHODS A total of 515 cases of pathologic stage I NSCLC were analyzed. The median follow-up time of surviving patients was 102 months. The following immunocytochemical markers were tested: blood group A and precursors of blood antigens; laminin receptor; c-erbB1/epidermal growth factor receptor (EGFR) and c-erbB2/Neu; BCl2; p53; and angiogenesis. Kaplan-Meier estimates of survival and time to recurrence were calculated for clinical variables and biologic markers using the Cox model for multivariate analysis. RESULTS The pathologic tumor extension (pT) represented the most powerful prognostic factor for survival (P = .0008) and time to recurrence (P = .0007). None of the immunocytochemical markers emerged as an independent predictive factor for survival. Bcl2-positive tumors showed a better time to recurrence (P = .03), but the difference lost statistical significance in the multivariate analysis. Of interest, in the group of 137 patients classified as pT1N0, both EGFR expression and nonangiogenic type of vascular pattern were associated with a poorer survival (P = .02). However, data derived from subset analysis must be interpreted cautiously. CONCLUSION Our findings do not support a relevant prognostic role of immunocytochemical markers in NSCLC. The evidence is not sufficient to alter clinical practice or even to restrict clinical trials of adjuvant treatments to predefined biologic subsets of patients.

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