Development and validation of a fast HPLC/photodiode array detection method for the measurement of voriconazole in human serum samples. A reference laboratory experience

2013 ◽  
Vol 31 (1) ◽  
pp. 23-28 ◽  
Author(s):  
Emilio Cendejas-Bueno ◽  
Juan L. Rodríguez-Tudela ◽  
Manuel Cuenca-Estrella ◽  
Alicia Gómez-López
Keyword(s):  
Human Serum ◽  
Detection Method ◽  
Reference Laboratory ◽  
Serum Samples ◽  
Photodiode Array Detection ◽  
Laboratory Experience ◽  
Human Serum Samples ◽  
Photodiode Array ◽  
Array Detection ◽  
Development And Validation

scholarly journals Development and Validation of a Reversed Phase High Performance Liquid Chromatography-Photodiode Array Detection Method for Simultaneous Identification and Quantification of Coumarin, Precocene-I, β-Caryophyllene Oxide, α-Humulene, and β-Caryophyllene in Ageratum Conyzoides Extracts and Essential Oils from Plants

2020 ◽  
Vol 103 (3) ◽  
pp. 857-864
Author(s):  
Sonal Shah ◽  
Tushar Dhanani ◽  
Sonu Sharma ◽  
Raghuraj Singh ◽  
Satyanshu Kumar ◽  
...  
Keyword(s):  
Essential Oils ◽  
Detection Method ◽  
Reversed Phase ◽  
Caryophyllene Oxide ◽  
Ageratum Conyzoides ◽  
Photodiode Array Detection ◽  
Photodiode Array ◽  
Development And Validation ◽  
Simultaneous Identification ◽  
Identification And Quantification

Abstract Background Ageratum conyzoides is an aromatic plant. It is considered as an invasive and cosmopolite weed, widely spread in tropical and subtropical regions. Phytochemicals such as benzopyrenes, flavonoids, and terpenoids are reported from A. conyzoides. Objective Development and validation of a reversed-phase HPLC-photodiode array (PDA) detection method for simultaneous identification and quantification of coumarin, precocene-I, β-caryophyllene oxide, α-humulene, and β-caryophyllene in extracts of A. conyzoides and essential oils was carried out. Methods Separation of analytes was achieved on a RP-18 (250 mm × 4.6 mm, 5 µm) column using a solvent system comprising of a mixture of acetonitrile and water with 0.05% trifluoroacetic acid in gradient elution mode at ambient temperature with flow rate of 1 mL/min. Results The retention time of coumarin, precocene-I, β-caryophyllene oxide, α-humulene, and β-caryophyllene was 4.38, 12.86, 20.10, 33.34, and 35.11 min, respectively. Limits of detection for coumarin, precocene-I, β-caryophyllene oxide, α-humulene, and β-caryophyllene were 2.5, 2.5, 2.5, 0.025, and 2.5 µg/mL, respectively. Similarly, LOQ were 10, 10, 10, 0.10, and 10 µg/mL for coumarin, precocene-I, β-caryophyllene oxide, α-humulene, and β- caryophyllene, respectively. Repeatabilities (RSD, %) values for intraday and interday precision for coumarin, precocene-I, β-caryophyllene oxide, α-humulene, and β-caryophyllene was 0.765–2.086 and 0.886–2.128; 0.879–1.672 and 0.979–1.825; 0.696–2.418 and 0.768–2.592; 1.728–2.362 and 1.965–2.378; 1.615–2.897 and 1.658–2.906, respectively. Conclusions The separation of five analytes was achieved within 50 min. The developed and validated HPLC-PDA method was successfully applied for identification and quantification of above five analytes in A. conyzoides extracts and essential oils. The method could be used for meeting the characterization criteria of phytoformulations.

Application of a fabric phase sorptive extraction-high performance liquid chromatography-photodiode array detection method for the trace determination of methyl paraben, propyl paraben and butyl paraben in cosmetic and environmental samples

2019 ◽  
Vol 11 (48) ◽  
pp. 6136-6145 ◽  
Author(s):  
Sumeyra Gülle ◽  
Halil Ibrahim Ulusoy ◽  
Abuzar Kabir ◽  
Angela Tartaglia ◽  
Kenneth G. Furton ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Environmental Samples ◽  
High Performance ◽  
Detection Method ◽  
Photodiode Array Detection ◽  
Sorptive Extraction ◽  
Photodiode Array ◽  
Array Detection

A new sample preparation and chromatographic separation method for methyl, propyl and butyl paraben in cosmetic and environmental samples has been developed, based on fabric phase sorptive extraction and high performance liquid chromatography-photodiode array detection.

Development and Validation of a UHPLC-PDA-MS Method for the Quantitative Analysis of Anthraquinones in Bulbine natalensis Extracts and Dietary Supplements

Planta Medica ◽  
2019 ◽  
Vol 86 (02) ◽  
pp. 144-150 ◽  
Author(s):  
Ji-Yeong Bae ◽  
Bharathi Avula ◽  
Yan-Hong Wang ◽  
Mei Wang ◽  
Zulfiqar Ali ◽  
...  
Keyword(s):  
Dietary Supplements ◽  
Electrospray Ionization ◽  
Analytical Method ◽  
Photodiode Array Detection ◽  
Plant Samples ◽  
Relative Standard ◽  
Photodiode Array ◽  
Array Detection ◽  
Development And Validation ◽  
Simple Economic

AbstractA UHPLC-photodiode array-MS method was developed and validated for the quantification of one chromone and six anthraquinone type of compounds from Bulbine natalensis plant samples and dietary supplements. Metabolites 1 –  7 were identified based on their retention times and electrospray ionization-MS spectra compared with a mix of previously isolated compounds. The quantification of 1 –  7 was based on photodiode array detection. The optimized separation was achieved using a CORTECS C18 column with a gradient of water/acetonitrile as the mobile phase. Seven compounds were separated within 15 minutes with detection limits of 50 pg on the column. The analytical method was validated for linearity, repeatability, accuracy, limits of detection, and limits of quantification. The relative standard deviations for intra- and inter-day experiments were less than 5% and the recovery efficiency was 98 – 101%. Nine dietary supplements labeled as containing B. natalensis were examined. Anthraquinone-type compounds were detected in only five out of nine dietary supplements, with the total amount ranging from 11.3 to 90.4 mg per daily dose. The analytical method is simple, economic, rapid, and can be applied for quality assessment of B. natalensis and dietary supplements. Electrospray ionization-MS was used for the identification of these compounds in plant samples and dietary products.

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