Altered gene expression in umbilical cord mononuclear cells in preterm infants with periventricular leukomalacia

2010 ◽  
Vol 86 (10) ◽  
pp. 665-667 ◽  
Author(s):  
Akihisa Okumura ◽  
Toshiyuki Yamamoto ◽  
Hiroyuki Kidokoro ◽  
Toru Kato ◽  
Tetsuo Kubota ◽  
...  
Keyword(s):  
Gene Expression ◽  
Preterm Infants ◽  
Umbilical Cord ◽  
Mononuclear Cells ◽  
Periventricular Leukomalacia ◽  
Altered Gene Expression ◽  
Altered Gene

scholarly journals Altered gene expression and metabolism in fetal umbilical cord mesenchymal stem cells correspond with differences in 5-month-old infant adiposity gain

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Peter R. Baker ◽  
Zachary W. Patinkin ◽  
Allison L. B. Shapiro ◽  
Becky A. de la Houssaye ◽  
Rachel C. Janssen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Altered Gene Expression ◽  
Altered Gene

Abstract TMP32: Transcriptome Changes of Brain Myeloid Cells After Ischemic Stroke in Type 2 Diabetic Mice

Stroke ◽  
2020 ◽  
Vol 51 (Suppl_1) ◽  
Author(s):  
Shunsuke Omodaka ◽  
Atsushi Kanoke ◽  
Suwei Wong ◽  
Dvir Aran ◽  
Jialing Liu
Keyword(s):  
Gene Expression ◽  
Antigen Processing ◽  
Mononuclear Cells ◽  
Myeloid Cells ◽  
Enrichment Analysis ◽  
Marker Genes ◽  
Sham Operation ◽  
Altered Gene Expression ◽  
Altered Gene

Introduction: Type 2 diabetes mellitus (T2DM) is associated with poor outcome after stroke. Brain myeloid cells are considered to play a pivotal role in modulating brain damage and recovery after stroke, but how myeloid response to stroke under diabetic condition is largely unclear. We used single-cell RNA sequencing (scRNA seq) to determine the transcriptome profiles of brain myeloid cells under diabetic and ischemic conditions using T2DM mouse model. Hypothesis: The altered gene expression in the brain myeloid cells under diabetic condition leads to the aggravation of ischemic brain injury. Methods: We performed scRNA seq in Percoll gradient-isolated brain mononuclear cells from middle-aged db/db and db/+ mice three days after direct middle cerebral artery occlusion (MCAO) or sham-operation. Clusters of brain myeloid cells were predominantly annotated as macrophages (mp) or microglia (mg) according to the expression of marker genes in each cell type. We identified DM-unique differentially expressed genes (DEGs) and stroke-unique DEGs, and assessed the biological role of these DEGs by enrichment analysis. Results: Myeloid cell population was increased in DM ( db/db without MCAO; mp 37.3% mg 10.6%) and stroke ( db/+ with MCAO; mp 26.2% mg 33.2%) group compared to control ( db/+ without MCAO; mp 3.4% mg 0.8%) group. In macrophages, 91 DM-unique (64 up- and 27 down-regulated) and 464 stroke-unique (458 up- and 6 down-regulated) DEGs were identified, whereas 258 stroke-unique (254 up- and 4 down-regulated) DEGs were identified in microglia. Enrichment analysis revealed that DM-unique down-regulated DEGs in macrophages were related to MHC class II antigen processing involved in Staphylococcus aureus infection pathway, indicating a possible relationship between immunosuppression and stroke aggravation in diabetes. DM-unique and stroke-unique up-regulated DEGs were related to oxidative phosphorylation, phagocytosis, and protein metabolism. Conclusions: The present study demonstrated altered gene expression profile and molecular network of brain myeloid cells in response to diabetic and ischemic conditions by scRNA seq, providing a clue to the underlying mechanism of the adverse effect of T2DM on stroke.

Molecular and Microscopic Analysis of Altered Gene Expression in Transgenic and Gene Targeted Mice

1996 ◽  
Vol 54 ◽  
pp. 12-13
Author(s):  
W. K. Jones ◽  
J. Robbins
Keyword(s):  
Gene Expression ◽  
Pulmonary Veins ◽  
Microscopic Analysis ◽  
Mouse Tissue ◽  
Adult Heart ◽  
Heavy Chains ◽  
Altered Gene Expression ◽  
Altered Gene ◽  
Pulmonary Myocardium

Two myosin heavy chains (MyHC) are expressed in the mammalian heart and are differentially regulated during development. In the mouse, the α-MyHC is expressed constitutively in the atrium. At birth, the β-MyHC is downregulated and replaced by the α-MyHC, which is the sole cardiac MyHC isoform in the adult heart. We have employed transgenic and gene-targeting methodologies to study the regulation of cardiac MyHC gene expression and the functional and developmental consequences of altered α-MyHC expression in the mouse.We previously characterized an α-MyHC promoter capable of driving tissue-specific and developmentally correct expression of a CAT (chloramphenicol acetyltransferase) marker in the mouse. Tissue surveys detected a small amount of CAT activity in the lung (Fig. 1a). The results of in situ hybridization analyses indicated that the pattern of CAT transcript in the adult heart (Fig. 1b, top panel) is the same as that of α-MyHC (Fig. 1b, lower panel). The α-MyHC gene is expressed in a layer of cardiac muscle (pulmonary myocardium) associated with the pulmonary veins (Fig. 1c). These studies extend our understanding of α-MyHC expression and delimit a third cardiac compartment.

Polymorphism of the MTHFR Gene is Associated with Altered Gene Expression in Colorectal Cancer

Endoscopy ◽  
2004 ◽  
Vol 36 (05) ◽  
Author(s):  
K Collins ◽  
GA Doherty ◽  
MR Sweeney ◽  
SM Byrne ◽  
AA Aftab ◽  
...  
Keyword(s):  
Gene Expression ◽  
Colorectal Cancer ◽  
Mthfr Gene ◽  
Altered Gene Expression ◽  
Altered Gene
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