Characterization of stem cell compartments in adult human liver

2009 ◽  
Vol 41 (3) ◽  
pp. A38-A39
Author(s):  
A. Cattaneo ◽  
L. Porretti ◽  
F. Colombo ◽  
R. Lopa ◽  
S. Mazzucchelli ◽  
...  
Keyword(s):  
Stem Cell ◽  
Human Liver ◽  
Adult Human ◽  
Cell Compartments

839 CHARACTERIZATION OF STEM CELL COMPARTMENTS IN ADULT HUMAN LIVER

2009 ◽  
Vol 50 ◽  
pp. S306
Author(s):  
L. Porretti ◽  
A. Cattaneo ◽  
F. Colombo ◽  
R. Lopa ◽  
S. Mazzucchelli ◽  
...  
Keyword(s):  
Stem Cell ◽  
Human Liver ◽  
Adult Human ◽  
Cell Compartments

scholarly journals Isolation and Characterization of a Stem Cell Population from Adult Human Liver

Stem Cells ◽  
2006 ◽  
Vol 24 (12) ◽  
pp. 2840-2850 ◽  
Author(s):  
Maria Beatriz Herrera ◽  
Stefania Bruno ◽  
Stefano Buttiglieri ◽  
Ciro Tetta ◽  
Stefano Gatti ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Population ◽  
Human Liver ◽  
Stem Cell Population ◽  
Adult Human ◽  
Isolation And Characterization

scholarly journals Simultaneous characterization of progenitor cell compartments in adult human liver

2009 ◽  
Vol 9999A ◽  
pp. NA-NA ◽  
Author(s):  
Laura Porretti ◽  
Alessandra Cattaneo ◽  
Federico Colombo ◽  
Raffaella Lopa ◽  
Giorgio Rossi ◽  
...  
Keyword(s):  
Human Liver ◽  
Progenitor Cell ◽  
Adult Human ◽  
Cell Compartments

C-kit (stem cell factor receptor) expression in normal and diseased adult human liver

2002 ◽  
Vol 36 ◽  
pp. 70-71
Author(s):  
Alberto Furlanetto ◽  
Roberto Togni ◽  
Stefano Negri ◽  
Romano Colombari
Keyword(s):  
Stem Cell ◽  
Stem Cell Factor ◽  
Human Liver ◽  
Receptor Expression ◽  
Adult Human

Isolation and Characterization of Stem Cell Clones from Adult Human Ligament

2009 ◽  
Vol 15 (9) ◽  
pp. 2625-2636 ◽  
Author(s):  
Weerachai Singhatanadgit ◽  
Nikolaos Donos ◽  
Irwin Olsen
Keyword(s):  
Stem Cell ◽  
Adult Human ◽  
Isolation And Characterization ◽  
Cell Clones

Molecular Characterization of Cultured Adult Human Liver Progenitor Cells

2010 ◽  
Vol 16 (5) ◽  
pp. 821-834 ◽  
Author(s):  
Justyna Jozefczuk ◽  
Harald Stachelscheid ◽  
Lukas Chavez ◽  
Ralf Herwig ◽  
Hans Lehrach ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Molecular Characterization ◽  
Human Liver ◽  
Adult Human

Isolation and Characterization of Adult Human Liver Progenitors from Ischemic Liver Tissue Derived from Therapeutic Hepatectomies

2009 ◽  
Vol 15 (7) ◽  
pp. 1633-1643 ◽  
Author(s):  
Harald Stachelscheid ◽  
Thomas Urbaniak ◽  
Alexander Ring ◽  
Berlind Spengler ◽  
Jörg C. Gerlach ◽  
...  
Keyword(s):  
Liver Tissue ◽  
Human Liver ◽  
Adult Human ◽  
Isolation And Characterization

Adult-Derived Human Liver Mesenchymal-Like Cells as a Potential Progenitor Reservoir of Hepatocytes?

2007 ◽  
Vol 16 (7) ◽  
pp. 717-728 ◽  
Author(s):  
Mustapha Najimi ◽  
Dung Ngoc Khuu ◽  
Philippe Antoine Lysy ◽  
Nawal Jazouli ◽  
Jorge Abarca ◽  
...  
Keyword(s):  
Stem Cell ◽  
Human Liver ◽  
Adult Stem Cells ◽  
Scid Mice ◽  
Differentiation Potential ◽  
Adult Human ◽  
Cell Source ◽  
Intrasplenic Transplantation

It is currently accepted that adult tissues may develop and maintain their own stem cell pools. Because of their higher safety profile, adult stem cells may represent an ideal candidate cell source to be used for liver cell therapies. We therefore evaluated the differentiation potential of mesenchymal-like cells isolated from adult human livers. Mesenchymal-like cells were isolated from enzymatically digested adult human liver and expanded in vitro. Cell characterization was performed using flow cytometry, RT-PCR, and immunofluorescence, whereas the differentiation potential was evaluated both in vitro after incubation with specific media and in vivo after intrasplenic transplantation of uPA+/+-SCID and SCID mice. Adult-derived human liver mesenchymal-like cells expressed both hepatic and mesenchymal markers among which albumin, CYP3A4, vimentin, and α-smooth muscle actin. In vitro differentiation studies demonstrated that these mesenchymal-like cells are preferentially determined to differentiate into hepatocyte-like cells. Ten weeks following intrasplenic transplantation into uPA+/+-SCID mice, recipient livers showed the presence of human hepatocytic cell nodules positive for human albumin, prealbumin, and α-fetoprotein. In SCID transplanted liver mice, human hepatocyte-like cells were mostly found near vascular structures 56 days posttransplantation. In conclusion, the ability of isolated adult-derived liver mesenchymal stem-like cells to proliferate and differentiate into hepatocyte-like cells both in vitro and in vivo leads to propose them as an attractive expandable cell source for stem cell therapy in human liver diseases.

scholarly journals Characterization of human spermatogonial stem cell markers in fetal, pediatric, and adult testicular tissues

Reproduction ◽  
2014 ◽  
Vol 148 (4) ◽  
pp. 417-427 ◽  
Author(s):  
Eran Altman ◽  
Pamela Yango ◽  
Radwa Moustafa ◽  
James F Smith ◽  
Peter C Klatsky ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cancer Treatment ◽  
Somatic Cells ◽  
Spermatogonial Stem Cell ◽  
Therapeutic Modality ◽  
Stem Cell Markers ◽  
Third Trimester ◽  
Membrane Markers ◽  
Adult Human

Autologous spermatogonial stem cell (SSC) transplantation is a potential therapeutic modality for patients with azoospermia following cancer treatment. For this promise to be realized, definitive membrane markers of prepubertal and adult human SSCs must be characterized in order to permit SSC isolation and subsequent expansion. This study further characterizes the markers of male gonocytes, prespermatogonia, and SSCs in humans. Human fetal, prepubertal, and adult testicular tissues were analyzed by confocal microscopy, fluorescence-activated cell sorting, and qRT-PCR for the expression of unique germ cell membrane markers. During male fetal development, THY1 and KIT (C-Kit) are transient markers of gonocytes but not in prespermatogonia and post-natal SSCs. Although KIT expression is detected in gonocytes, THY1 expression is also detected in the somatic component of the fetal testes in addition to gonocytes. In the third trimester of gestation, THY1 expression shifts exclusively to the somatic cells of the testes where it continues to be detected only in the somatic cells postnatally. In contrast, SSEA4 expression was only detected in the gonocytes, prespermatogonia, SSCs, and Sertoli cells of the fetal and prepubertal testes. After puberty, SSEA4 expression can only be detected in primitive spermatogonia. Thus, although THY1 and KIT are transient markers of gonocytes, SSEA4 is the only common membrane marker of gonocytes, prespermatogonia, and SSCs from fetal through adult human development. This finding is essential for the isolation of prepubertal and adult SSCs, which may someday permit fertility preservation and reversal of azoospermia following cancer treatment.

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