scholarly journals Human amniotic membrane as an alternative source of stem cells for regenerative medicine

2011 ◽  
Vol 81 (3) ◽  
pp. 162-171 ◽  
Author(s):  
Silvia Díaz-Prado ◽  
Emma Muiños-López ◽  
Tamara Hermida-Gómez ◽  
Claudia Cicione ◽  
M. Esther Rendal-Vázquez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Regenerative Medicine ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
Alternative Source

scholarly journals Human Amniotic Membrane as a Matrix for Endothelial Differentiation of VEGF-Treated Dental Stem Cells

2019 ◽  
Vol 12 (6) ◽  
pp. 599-613 ◽  
Author(s):  
Siti Nurnasihah Md Hashim ◽  
Muhammad Fuad Hilmi Yusof ◽  
Wafa’ Zahari ◽  
Hamshawagini Chandra ◽  
Khairul Bariah Ahmad Amin Noordin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Amniotic Membrane ◽  
Endothelial Differentiation ◽  
Human Amniotic Membrane ◽  
Dental Stem Cells

Effects of Preservation Methods in the Composition of the Placental and Reflected Regions of the Human Amniotic Membrane

2021 ◽  
pp. 1-11
Author(s):  
Jéssica Tereza Guedes de Oliveira Moraes ◽  
Maíra Maftoum Costa ◽  
Paula Cristina Santos Alves ◽  
Luciana Barros Sant’Anna
Keyword(s):  
Regenerative Medicine ◽  
Amniotic Membrane ◽  
Structural Integrity ◽  
Storage Period ◽  
Soluble Proteins ◽  
Control Group ◽  
Human Amniotic Membrane ◽  
Glycerol Solution ◽  
Preservation Methods ◽  
Preservation Method

The human amniotic membrane (AM) is emerging as an interesting biomaterial for regenerative medicine due to its biological and mechanical proprieties. The beneficial effects of the AM are probably related to its bioactive factors produced by local cells and stored in the stromal matrix. However, the search for a preservation method capable of preserving AM properties remains a challenge. The aim of this study was to evaluate important features of 2 anatomical regions of the human AM (reflected and placental amnion) after different preservation methods. For this purpose, human placentas were harvested and processed for AM isolation and storage at 2 different conditions: room temperature for 18 h in DMEM (fresh AM) and −80°C in DMEM/glycerol solution for 30 days (cryopreserved AM). After the storage period, the structural integrity of the membrane was assessed by histological and Picrosirius polarization analysis, cellular viability analysis was performed using the MTT assay, and the soluble proteins were quantified with the Qubit Protein Assay Kit. Both preservation protocols reduced the cell viability, mainly in the placental amnion region of the AM, but preserved the morphology of epithelial and stromal layers, as well as the organization and distribution of collagen fibers. There was a reduction in soluble proteins only in fresh AM. Importantly, the cryopreserved AM group presented the same concentration as the control group. In conclusion, the cryopreservation using DMEM/glycerol was ideal for preserving the structural integrity and soluble protein content, indicating the feasibility of this method in preserving AM for its use in regenerative medicine.

scholarly journals Anti-Inflammatory and Anti-Fibrotic Effects of Human Amniotic Membrane Mesenchymal Stem Cells and Their Potential in Corneal Repair

2018 ◽  
Vol 7 (12) ◽  
pp. 906-917 ◽  
Author(s):  
Alejandro Navas ◽  
Fátima Sofía Magaña-Guerrero ◽  
Alfredo Domínguez-López ◽  
César Chávez-García ◽  
Graciela Partido ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
Anti Inflammatory

scholarly journals Health Span-Extending Activity of Human Amniotic Membrane- and Adipose Tissue-Derived Stem Cells in F344 Rats

2015 ◽  
Vol 4 (10) ◽  
pp. 1144-1154 ◽  
Author(s):  
Dajeong Kim ◽  
Jangbeen Kyung ◽  
Dongsun Park ◽  
Ehn-Kyoung Choi ◽  
Kwang Sei Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
Health Span ◽  
F344 Rats

Mesenchymal stem cells seeded on a human amniotic membrane improve liver regeneration and mouse survival after extended hepatectomy

2017 ◽  
Vol 12 (4) ◽  
pp. 1062-1073 ◽  
Author(s):  
Aure Despeyroux ◽  
Cédric Duret ◽  
Claire Gondeau ◽  
Esther Perez‐Gracia ◽  
Lisa Chuttoo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Liver Regeneration ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
Extended Hepatectomy

Hepatic differentiation of pluripotent stem cells

2009 ◽  
Vol 390 (10) ◽  
Author(s):  
Komal Loya ◽  
Reto Eggenschwiler ◽  
Kinarm Ko ◽  
Malte Sgodda ◽  
Francoise André ◽  
...  
Keyword(s):  
Stem Cells ◽  
Regenerative Medicine ◽  
Pluripotent Stem Cells ◽  
Ethical Issues ◽  
Es Cells ◽  
Embryonic Stem ◽  
Hepatic Differentiation ◽  
Alternative Source ◽  
Organ Specific

Abstract In regenerative medicine pluripotent stem cells are considered to be a valuable self-renewing source for therapeutic cell transplantations, given that a functional organ-specific phenotype can be acquired by in vitro differentiation protocols. Furthermore, derivatives of pluripotent stem cells that mimic fetal progenitor stages could serve as an important tool to analyze organ development with in vitro approaches. Because of ethical issues regarding the generation of human embryonic stem (ES) cells, other sources for pluripotent stem cells are intensively studied. Like in less developed vertebrates, pluripotent stem cells can be generated from the female germline even in mammals, via parthenogenetic activation of oocytes. Recently, testis-derived pluripotent stem cells were derived from the male germline. Therefore, we compared two different hepatic differentiation approaches and analyzed the generation of definitive endoderm progenitor cells and their further maturation into a hepatic phenotype using murine parthenogenetic ES cells, germline-derived pluripotent stem cells, and ES cells. Applying quantitative RT-PCR, both germline-derived pluripotent cell lines show similar differentiation capabilities as normal murine ES cells and can be considered an alternative source for pluripotent stem cells in regenerative medicine.

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