Monitoring stem cell proliferation and differentiation in primary midgut cell cultures from Heliothis virescens larvae using flow cytometry

2011 ◽  
Vol 81 (3) ◽  
pp. 192-198 ◽  
Author(s):  
A. Castagnola ◽  
S. Eda ◽  
J.L. Jurat-Fuentes
Keyword(s):  
Flow Cytometry ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Cell Cultures ◽  
Heliothis Virescens ◽  
Midgut Cell ◽  
Stem Cell Proliferation ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

Cannabinoid receptor signaling in progenitor/stem cell proliferation and differentiation

2013 ◽  
Vol 52 (4) ◽  
pp. 633-650 ◽  
Author(s):  
Ismael Galve-Roperh ◽  
Valerio Chiurchiù ◽  
Javier Díaz-Alonso ◽  
Monica Bari ◽  
Manuel Guzmán ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Stem Cell ◽  
Cannabinoid Receptor ◽  
Receptor Signaling ◽  
Stem Cell Proliferation ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

scholarly journals MicroRNA let-7b regulates neural stem cell proliferation and differentiation by targeting nuclear receptor TLX signaling

2010 ◽  
Vol 107 (5) ◽  
pp. 1876-1881 ◽  
Author(s):  
Chunnian Zhao ◽  
GuoQiang Sun ◽  
Shengxiu Li ◽  
Ming-Fei Lang ◽  
Su Yang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Cyclin D1 ◽  
Neural Stem Cell ◽  
Neural Differentiation ◽  
Stem Cell Proliferation ◽  
Neural Stem Cell Proliferation ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

Neural stem cell self-renewal and differentiation is orchestrated by precise control of gene expression involving nuclear receptor TLX. Let-7b, a member of the let-7 microRNA family, is expressed in mammalian brains and exhibits increased expression during neural differentiation. However, the role of let-7b in neural stem cell proliferation and differentiation remains unknown. Here we show that let-7b regulates neural stem cell proliferation and differentiation by targeting the stem cell regulator TLX and the cell cycle regulator cyclin D1. Overexpression of let-7b led to reduced neural stem cell proliferation and increased neural differentiation, whereas antisense knockdown of let-7b resulted in enhanced proliferation of neural stem cells. Moreover, in utero electroporation of let-7b to embryonic mouse brains led to reduced cell cycle progression in neural stem cells. Introducing an expression vector of Tlx or cyclin D1 that lacks the let-7b recognition site rescued let-7b-induced proliferation deficiency, suggesting that both TLX and cyclin D1 are important targets for let-7b-mediated regulation of neural stem cell proliferation. Let-7b, by targeting TLX and cyclin D1, establishes an efficient strategy to control neural stem cell proliferation and differentiation.

Sign in / Sign up

Export Citation Format

Share Document