Isolation of multipotent stem cells in human periodontal ligament using stage-specific embryonic antigen-4

2010 ◽  
Vol 79 (2) ◽  
pp. 74-83 ◽  
Author(s):  
Noriaki Kawanabe ◽  
Satoko Murata ◽  
Kaoru Murakami ◽  
Yoshihito Ishihara ◽  
Satoru Hayano ◽  
...  
Keyword(s):  
Stem Cells ◽  
Periodontal Ligament ◽  
Multipotent Stem Cells ◽  
Embryonic Antigen ◽  
Stage Specific Embryonic Antigen

Stage-Specific Embryonic Antigen 4 in Wharton's Jelly–Derived Mesenchymal Stem Cells Is Not a Marker for Proliferation and Multipotency

2014 ◽  
Vol 20 (7-8) ◽  
pp. 1314-1324 ◽  
Author(s):  
Haiping He ◽  
Tokiko Nagamura-Inoue ◽  
Hajime Tsunoda ◽  
Miki Yuzawa ◽  
Yuki Yamamoto ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Wharton’S Jelly ◽  
Wharton's Jelly ◽  
Embryonic Antigen ◽  
Stage Specific Embryonic Antigen

Cell origin in the macula flava of the human newborn vocal fold

2016 ◽  
Vol 130 (7) ◽  
pp. 650-655 ◽  
Author(s):  
K Sato ◽  
S Chitose ◽  
T Kurita ◽  
H Umeno
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Vocal Fold ◽  
Collagen Type ◽  
Collagen Type I ◽  
Telomerase Reverse Transcriptase ◽  
Type I ◽  
Embryonic Antigen ◽  
Cluster Of Differentiation ◽  
Stage Specific Embryonic Antigen

AbstractBackground:There is growing evidence to suggest that cells in the maculae flavae are tissue stem cells of the human vocal fold and maculae flavae are a stem cell niche.Methods:Three newborn vocal folds were investigated. Immunoreactivity to antibodies directed to cytokeratin, desmin, glial fibrillary acidic protein, vimentin, cluster of differentiation 34, cluster of differentiation 45, collagen type I, telomerase reverse transcriptase, SOX17 and stage-specific embryonic antigen 3 was investigated.Results:The cells in the newborn maculae flavae expressed haematopoietic markers (cluster of differentiation 34, cluster of differentiation 45) and collagen type I, which are the major makers of bone marrow derived circulating fibrocytes. The cells expressed epithelium, muscle, neural and mesenchymal cell associated proteins, and endodermal marker, indicating that they are undifferentiated and express proteins of all three germ layers. The cells also expressed stage-specific embryonic antigen 3 and telomerase reverse transcriptase.Conclusion:The cells in the newborn maculae flavae are undifferentiated cells arising from the differentiation of bone marrow cells. The results of this study are consistent with the hypothesis that the cells in maculae flavae are tissue stem cells.

Stage-specific embryonic antigen-4 identifies human dental pulp stem cells

2012 ◽  
Vol 318 (5) ◽  
pp. 453-463 ◽  
Author(s):  
Noriaki Kawanabe ◽  
Satoko Murata ◽  
Hiroaki Fukushima ◽  
Yoshihito Ishihara ◽  
Takeshi Yanagita ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp ◽  
Embryonic Antigen ◽  
Stage Specific Embryonic Antigen

Identification of multipotent stem cells from adult dog periodontal ligament

2012 ◽  
Vol 120 (4) ◽  
pp. 303-310 ◽  
Author(s):  
Wen-Jun Wang ◽  
Yu-Ming Zhao ◽  
Bi-Chen Lin ◽  
Jie Yang ◽  
Li-Hong Ge
Keyword(s):  
Stem Cells ◽  
Periodontal Ligament ◽  
Multipotent Stem Cells

Differentiation of bone marrow-derived stage-specific embryonic antigen 1 positive pluripotent stem cells into male germ cells

2016 ◽  
Vol 80 (4) ◽  
pp. 430-440 ◽  
Author(s):  
Reza Shirazi ◽  
Amir Hassan Zarnani ◽  
Masoud Soleimani ◽  
Karim Nayernia ◽  
Iraj Ragerdi Kashani
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Germ Cells ◽  
Pluripotent Stem Cells ◽  
Male Germ Cells ◽  
Embryonic Antigen ◽  
Stage Specific Embryonic Antigen

Isolation of multipotent stem cells from adult rat periodontal ligament by neurosphere-forming culture system

2007 ◽  
Vol 357 (4) ◽  
pp. 917-923 ◽  
Author(s):  
Wanida Techawattanawisal ◽  
Kenichi Nakahama ◽  
Motohiro Komaki ◽  
Mayumi Abe ◽  
Yuzo Takagi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Periodontal Ligament ◽  
Culture System ◽  
Multipotent Stem Cells ◽  
Adult Rat

scholarly journals ISOLATION OF AMNIOTIC FLUID MESENCHYMAL STEM CELLS (AF-MSCs) OBTAINED FROM CAESAREAN SECTIONS

2019 ◽  
Vol 2 (1) ◽  
pp. 1-9
Author(s):  
Bobby Indra Utama
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Amniotic Fluid ◽  
Pelvic Organ ◽  
Rt Pcr ◽  
Third Trimester ◽  
Amniotic Cavity ◽  
Hla Dr ◽  
Embryonic Antigen ◽  
Stage Specific Embryonic Antigen

Amniotic fluid is a liquid that fills the amniotic cavity which has defense and nutritional functions in fetal development. Human aterm amniotic fluid can be an ideal alternative as a source of mesenchymal stem cells, originating from the neonate. Preclinical studies of second and third trimester amnion fluid cells confirmed the number of potential donors from this wasted material. In several studies, AF-MSCs express mesenchymal markers such as CD90, CD73 (SH3, SH), CD105 (SH2), CD29, CD166, CD49e, CD58 and CD44 (MHC class I). These cells also express HLA-ABC antigens, CD 34, CD 45 which are hematopoietic markers, and endothelial CD31 markers. There is no expression of CD10, CD11b, CD14, CD34, CD117, EMA and HLA-DR, DP, DQ antigens. Most of AF-MSCs have pluripotent properties which are characterized by the discovery of octamer binding protein 3/4 (Oct-3/4), transcription factors Nanog (Nanog), and stage-specific embryonic antigen 4 (SSEA-4) on RT-PCR examination. From this study, 8 million cells was isolated. These cells will be used for research on pelvic organ prolapse therapy by using AF-MSCs. AF-MSCs isolation totally takes 6 weeks. From 1 flask, 2 million of stem cells was obtained. Keywords: amniotic fluid, AF-MSCs

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