Gene expression profiles of human inner cell mass cells and embryonic stem cells

2009 ◽  
Vol 78 (1) ◽  
pp. 18-23 ◽  
Author(s):  
Renee A. Reijo Pera ◽  
Christopher DeJonge ◽  
Nancy Bossert ◽  
Mylene Yao ◽  
Jean Yee Hwa Yang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Inner Cell Mass ◽  
Expression Profiles ◽  
Cell Mass ◽  
Embryonic Stem ◽  
Gene Expression Profiles

scholarly journals Comparison of gene expression profiles in erythroid-like cells derived from mouse embryonic stem cells differentiated in simple and co-culture systems

2008 ◽  
Vol 83 (2) ◽  
pp. 109-115 ◽  
Author(s):  
Mandana B. Boroujeni ◽  
Mojdeh Salehnia ◽  
Mojtaba R. Valojerdi ◽  
Seyed J. Mowla ◽  
Mehdi Forouzandeh ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Expression Profiles ◽  
Embryonic Stem ◽  
Gene Expression Profiles ◽  
Mouse Embryonic Stem Cells ◽  
Culture Systems

scholarly journals Gene expression profiles during early differentiation of mouse embryonic stem cells

2009 ◽  
Vol 9 (1) ◽  
Author(s):  
Fiona C Mansergh ◽  
Carl S Daly ◽  
Anna L Hurley ◽  
Michael A Wride ◽  
Susan M Hunter ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Expression Profiles ◽  
Embryonic Stem ◽  
Gene Expression Profiles ◽  
Mouse Embryonic Stem Cells ◽  
Early Differentiation

scholarly journals Gene expression profiles in CHA3 and CHA4 human embryonic stem cells and embryoid bodies

2011 ◽  
Vol 31 (4) ◽  
pp. 315-326 ◽  
Author(s):  
Sung-Hwan Moon ◽  
Sung-Whan Kim ◽  
Jong Soo Kim ◽  
Soon-Jung Park ◽  
Jeong Tae Do ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Expression Profiles ◽  
Embryonic Stem ◽  
Gene Expression Profiles ◽  
Embryoid Bodies

scholarly journals Mesenchymal Stem/Stromal-Like Cells from Diploid and Triploid Human Embryonic Stem Cells Display Different Gene Expression Profiles

2021 ◽  
Vol 25 (2) ◽  
pp. 99-105
Author(s):  
Mahdieh Javidpou ◽  
Seyed-Morteza Seifati ◽  
Ehsan Farashahi-Yazd ◽  
Fatemeh Hajizadeh-Tafti ◽  
Jalal Golzadeh ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Expression Profiles ◽  
Embryonic Stem ◽  
Gene Expression Profiles

scholarly journals Differentiation of Human Embryonic Stem Cells into Neuron, Cholinergic, and Glial Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Kimia Hosseini ◽  
Emilia Lekholm ◽  
Aikeremu Ahemaiti ◽  
Robert Fredriksson
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Glial Cells ◽  
Human Embryonic Stem Cells ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Embryonic Stem ◽  
Cholinergic Neurons ◽  
Neuronal Cultures ◽  
Short Period

Human embryonic stem cells (hESCs) are pluripotent cells, capable of differentiation into different cellular lineages given the opportunity. Derived from the inner cell mass of blastocysts in early embryonic development, the cell self-renewal ability makes them a great tool for regenerative medicine, and there are different protocols available for maintaining hESCs in their undifferentiated state. In addition, protocols for differentiation into functional human neural stem cells (hNSCs), which have the potential for further differentiation into various neural cell types, are available. However, many protocols are time-consuming and complex and do not always fit for purpose. In this study, we carefully combined, optimized, and developed protocols for differentiation of hESCs into adherent monolayer hNSCs over a short period of time, with the possibility of both expansion and freezing. Moreover, the method details further differentiation into neurons, cholinergic neurons, and glial cells in a simple, single step by step protocol. We performed immunocytochemistry, qPCR, and electrophysiology to examine the expression profile and characteristics of the cells to verify cell lineage. Using presented protocols, the creation of neuronal cultures, cholinergic neurons, and a mixed culture of astrocytes and oligodendrocytes can be completed within a three-week time period.

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