scholarly journals Data on cell viability of human lung fibroblasts treated with polyphenols-rich extract from Plinia trunciflora (O. Berg) Kausel)

Data in Brief ◽  
2016 ◽  
Vol 6 ◽  
pp. 728-731 ◽  
Author(s):  
Caroline Calloni ◽  
Luciana Fernandes Silva Santos ◽  
Luana Soares Martínez ◽  
Mirian Salvador
Keyword(s):  
Cell Viability ◽  
Human Lung ◽  
Lung Fibroblasts ◽  
Human Lung Fibroblasts

scholarly journals Silver Nanoparticles Alter Cell Viability Ex Vivo and in Vitro and Induce Proinflammatory Effects in Human Lung Fibroblasts

Nanomaterials ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1868
Author(s):  
Anna Löfdahl ◽  
Andreas Jern ◽  
Samuel Flyman ◽  
Monica Kåredal ◽  
Hanna L Karlsson ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cell Viability ◽  
Human Lung ◽  
Inflammatory Responses ◽  
Ex Vivo ◽  
Lung Fibroblasts ◽  
Human Lung Fibroblasts ◽  
Metabolic Activities ◽  
Precision Cut Lung Slices

Silver nanoparticles (AgNPs) are commonly used in commercial and medical applications. However, AgNPs may induce toxicity, extracellular matrix (ECM) changes and inflammatory responses. Fibroblasts are key players in remodeling processes and major producers of the ECM. The aims of this study were to explore the effect of AgNPs on cell viability, both ex vivo in murine precision cut lung slices (PCLS) and in vitro in human lung fibroblasts (HFL-1), and immunomodulatory responses in fibroblasts. PCLS and HFL-1 were exposed to AgNPs with different sizes, 10 nm and 75 nm, at concentrations 2 µg/mL and 10 μg/mL. Changes in synthesis of ECM proteins, growth factors and cytokines were analyzed in HFL-1. Ag10 and Ag75 affected cell viability, with significantly reduced metabolic activities at 10 μg/mL in both PCLS and HFL-1 after 48 h. AgNPs significantly increased procollagen I synthesis and release of IL-8, prostaglandin E2, RANTES and eotaxin, whereas reduced IL-6 release was observed in HFL-1 after 72 h. Our data indicate toxic effects of AgNP exposure on cell viability ex vivo and in vitro with altered procollagen and proinflammatory cytokine secretion in fibroblasts over time. Hence, careful characterizations of AgNPs are of importance, and future studies should include timepoints beyond 24 h.

scholarly journals New Insights into the Cell Death Signaling Pathways Triggered by Long-Term Exposure to Silicon-Based Quantum Dots in Human Lung Fibroblasts

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 323
Author(s):  
Miruna S. Stan ◽  
Smaranda Badea ◽  
Anca Hermenean ◽  
Hildegard Herman ◽  
Bogdan Trica ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Cell Viability ◽  
Human Lung ◽  
Control Level ◽  
Lung Fibroblasts ◽  
Population Doubling ◽  
Blue Staining ◽  
Human Lung Fibroblasts ◽  
Silicon Based

This report is the first research study that aims to explore the molecular mechanisms involved in the in vitro pulmonary cytotoxicity triggered by long-term exposure to silicon-based quantum dots (QDs). Human lung fibroblasts (MRC-5 cell line) were exposed to 5 µg/mL silicon-based QDs for 5 weeks and the concentration was increased up to 40 µg/mL QDs during the next 4 weeks. Cell viability and population doubling level were calculated based on Trypan blue staining. The expression levels of proteins were established by Western blotting and the telomeres’ length was determined through Southern blotting. Prolonged exposure of lung fibroblasts to QDs reduced the cell viability by 10% compared to untreated cells. The level of p53 and apoptosis-inducing factor (AIF) expression increased during the exposure, the peak intensity being registered after the seventh week. The expressions of autophagy-related proteins, Beclin-1 and LC-3, were higher compared to untreated cells. Regarding the protein expression of Nrf-2, a progressive decrease was noticed, suggesting the downregulation of a cytoprotective response to oxidative stress. In contrast, the heat shock proteins’ (HSPs) expression was increased or maintained near the control level during QDs exposure in order to promote cell survival. Furthermore, the telomeres’ length was not reduced during this exposure, indicating that QDs did not induce cellular senescence. In conclusion, our study shows that silicon-based QDs triggered the activation of apoptotic and autophagy pathways and downregulation of survival signaling molecules as an adaptive response to cellular stress which was not associated with telomeres shortening.

scholarly journals Fenofibrate inhibits TGF‐β‐induced myofibroblast differentiation and activation in human lung fibroblasts in vitro

FEBS Open Bio ◽  
2021 ◽  
Author(s):  
Ryota Kikuchi ◽  
Yuki Maeda ◽  
Takao Tsuji ◽  
Kazuhiro Yamaguchi ◽  
Shinji Abe ◽  
...  
Keyword(s):  
Human Lung ◽  
Lung Fibroblasts ◽  
Myofibroblast Differentiation ◽  
Human Lung Fibroblasts

scholarly journals Procollagen production and procollagen messenger RNA levels and activity in human lung fibroblasts during periods of rapid and stationary growth.

1981 ◽  
Vol 256 (6) ◽  
pp. 3135-3140
Author(s):  
P. Tolstoshev ◽  
R.A. Berg ◽  
S.I. Rennard ◽  
K.H. Bradley ◽  
B.C. Trapnell ◽  
...  
Keyword(s):  
Messenger Rna ◽  
Human Lung ◽  
Lung Fibroblasts ◽  
Stationary Growth ◽  
Human Lung Fibroblasts ◽  
Rna Levels
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