scholarly journals High-throughput imaging method for direct assessment of GM1 ganglioside levels in mammalian cells

Data in Brief ◽  
2016 ◽  
Vol 6 ◽  
pp. 1016-1022 ◽  
Author(s):  
Walter Acosta ◽  
Reid Martin ◽  
David N. Radin ◽  
Carole L. Cramer
Keyword(s):  
High Throughput ◽  
Mammalian Cells ◽  
Imaging Method ◽  
Gm1 Ganglioside ◽  
Direct Assessment ◽  
High Throughput Imaging

A Semi-high-throughput Imaging Method and Data Visualization Toolkit to Analyze C. elegans Embryonic Development

10.3791/60362 ◽  
2019 ◽  
Author(s):  
Renat N. Khaliullin ◽  
Jeffrey M. Hendel ◽  
Adina Gerson-Gurwitz ◽  
Shaohe Wang ◽  
Stacy D. Ochoa ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Data Visualization ◽  
High Throughput ◽  
Imaging Method ◽  
C Elegans ◽  
Visualization Toolkit ◽  
High Throughput Imaging

scholarly journals HiHiMap: single-cell quantitation of histones and histone posttranslational modifications across the cell cycle by high-throughput imaging

2017 ◽  
Vol 28 (17) ◽  
pp. 2290-2302 ◽  
Author(s):  
Linda Zane ◽  
Fleur Chapus ◽  
Gianluca Pegoraro ◽  
Tom Misteli
Keyword(s):  
Cell Cycle ◽  
High Throughput ◽  
Posttranslational Modifications ◽  
Single Cells ◽  
Histone Variants ◽  
Imaging Method ◽  
Histone Ptms ◽  
Core Histones ◽  
High Throughput Imaging ◽  
High Throughput Study

We describe High-throughput Histone Mapping (HiHiMap), a high-throughput imaging method to measure histones and histone posttranslational modifications (PTMs) in single cells. HiHiMap uses imaging-based quantification of DNA and cyclin A to stage individual cells in the cell cycle to determine the levels of histones or histone PTMs in each stage of the cell cycle. As proof of principle, we apply HiHiMap to measure the level of 21 core histones, histone variants, and PTMs in primary, immortalized, and transformed cells. We identify several histone modifications associated with oncogenic transformation. HiHiMap allows the rapid, high-throughput study of histones and histone PTMs across the cell cycle and the study of subpopulations of cells.

scholarly journals Real-time imaging of cellular forces using optical interference

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Andrew T. Meek ◽  
Nils M. Kronenberg ◽  
Andrew Morton ◽  
Philipp Liehm ◽  
Jan Murawski ◽  
...  
Keyword(s):  
Cell Culture ◽  
Real Time ◽  
High Throughput ◽  
Mechanical Activity ◽  
Dynamic Processes ◽  
Imaging Method ◽  
Neonatal Cardiomyocytes ◽  
A Cell ◽  
Cellular Forces ◽  
Time Acquisition

AbstractImportant dynamic processes in mechanobiology remain elusive due to a lack of tools to image the small cellular forces at play with sufficient speed and throughput. Here, we introduce a fast, interference-based force imaging method that uses the illumination of an elastic deformable microcavity with two rapidly alternating wavelengths to map forces. We show real-time acquisition and processing of data, obtain images of mechanical activity while scanning across a cell culture, and investigate sub-second fluctuations of the piconewton forces exerted by macrophage podosomes. We also demonstrate force imaging of beating neonatal cardiomyocytes at 100 fps which reveals mechanical aspects of spontaneous oscillatory contraction waves in between the main contraction cycles. These examples illustrate the wider potential of our technique for monitoring cellular forces with high throughput and excellent temporal resolution.

scholarly journals High-throughput imaging of fresh-frozen plant reproductive samples in a variable pressure SEM

MethodsX ◽  
2021 ◽  
pp. 101392
Author(s):  
Haydee E. Laza ◽  
Bo Zhao ◽  
Mary Hastert ◽  
Paxton Payton ◽  
Junping Chen
Keyword(s):  
High Throughput ◽  
Variable Pressure ◽  
Fresh Frozen ◽  
High Throughput Imaging
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