Motor usage imprints microtubule stability along the shaft

2021 ◽  
Author(s):  
Mireia Andreu-Carbó ◽  
Simon Fernandes ◽  
Marie-Claire Velluz ◽  
Karsten Kruse ◽  
Charlotte Aumeier
Keyword(s):  
Microtubule Stability

scholarly journals Inhibition of cell expansion enhances cortical microtubule stability in the root apex of Arabidopsis thaliana

2021 ◽  
Vol 28 (1) ◽  
Author(s):  
Veronica Giourieva ◽  
Emmanuel Panteris
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Wall ◽  
Cell Expansion ◽  
Cortical Microtubule ◽  
Root Apex ◽  
Cellulose Synthase ◽  
Cellulose Biosynthesis ◽  
Cortical Microtubules ◽  
Wild Type ◽  
Microtubule Stability

Abstract Background Cortical microtubules regulate cell expansion by determining cellulose microfibril orientation in the root apex of Arabidopsis thaliana. While the regulation of cell wall properties by cortical microtubules is well studied, the data on the influence of cell wall to cortical microtubule organization and stability remain scarce. Studies on cellulose biosynthesis mutants revealed that cortical microtubules depend on Cellulose Synthase A (CESA) function and/or cell expansion. Furthermore, it has been reported that cortical microtubules in cellulose-deficient mutants are hypersensitive to oryzalin. In this work, the persistence of cortical microtubules against anti-microtubule treatment was thoroughly studied in the roots of several cesa mutants, namely thanatos, mre1, any1, prc1-1 and rsw1, and the Cellulose Synthase Interacting 1 protein (csi1) mutant pom2-4. In addition, various treatments with drugs affecting cell expansion were performed on wild-type roots. Whole mount tubulin immunolabeling was applied in the above roots and observations were performed by confocal microscopy. Results Cortical microtubules in all mutants showed statistically significant increased persistence against anti-microtubule drugs, compared to those of the wild-type. Furthermore, to examine if the enhanced stability of cortical microtubules was due to reduced cellulose biosynthesis or to suppression of cell expansion, treatments of wild-type roots with 2,6-dichlorobenzonitrile (DCB) and Congo red were performed. After these treatments, cortical microtubules appeared more resistant to oryzalin, than in the control. Conclusions According to these findings, it may be concluded that inhibition of cell expansion, irrespective of the cause, results in increased microtubule stability in A. thaliana root. In addition, cell expansion does not only rely on cortical microtubule orientation but also plays a regulatory role in microtubule dynamics, as well. Various hypotheses may explain the increased cortical microtubule stability under decreased cell expansion such as the role of cell wall sensors and the presence of less dynamic cortical microtubules.

scholarly journals Erratum: Corrigendum: Megakaryocyte-specific Profilin1-deficiency alters microtubule stability and causes a Wiskott–Aldrich syndrome-like platelet defect

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Markus Bender ◽  
Simon Stritt ◽  
Paquita Nurden ◽  
Judith M.M. van Eeuwijk ◽  
Barbara Zieger ◽  
...  
Keyword(s):  
Wiskott Aldrich Syndrome ◽  
Microtubule Stability

The control of microtubule stability in vitro and in transfected cells by MAP1B and SCG10

2006 ◽  
Vol 63 (11) ◽  
pp. 681-695 ◽  
Author(s):  
Percy Bondallaz ◽  
Anne Barbier ◽  
Sophia Soehrman ◽  
Gabriele Grenningloh ◽  
Beat M. Riederer
Keyword(s):  
Transfected Cells ◽  
Microtubule Stability

Altering microtubule stability affects microtubule clearance and nuclear extrusion during erythropoiesis

2019 ◽  
Vol 234 (11) ◽  
pp. 19833-19841 ◽  
Author(s):  
Songbo Xie ◽  
Bing Yan ◽  
Jie Feng ◽  
Yuhan Wu ◽  
Na He ◽  
...  
Keyword(s):  
Microtubule Stability

scholarly journals MACC1 Contributes to the Development of Osteosarcoma Through Regulation of the HGF/c-Met Pathway and Microtubule Stability

2020 ◽  
Vol 8 ◽  
Author(s):  
Jia Wen ◽  
Yi Xie ◽  
Yingqiang Zhang ◽  
Jiazhen Li ◽  
Jiaping Li ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Tumor Growth ◽  
Signaling Pathway ◽  
Normal Tissues ◽  
Microtubule Stability ◽  
Promising Therapeutic Target ◽  
Tumor Growth And Metastasis ◽  
Hepatocyte Growth ◽  
Proliferative Ability

Osteosarcoma (OS) is the most prevalent human bone malignancy, and presents a global annual morbidity of approximately five cases per million. Notably, precise and efficient targeted therapy has become the most promising strategy for the treatment of OS; however, there is still an urgent need for the identification of suitable therapeutic targets. Metastasis-associated in colon cancer 1 (MACC1) was first identified in colon tumors by differential display RT-PCR, and was shown to be involved in the regulation of colon tumor growth and metastasis through the hepatocyte growth factor (HGF)/c-Met signaling pathway. Additionally, MACC1 overexpression has been reported to induce the growth of several types of cancers, including glioblastoma multiforme and gastric cancer. However, whether MACC1 also plays a role in the progression of OS remains unclear. In this study, we found that MACC1 was highly expressed in human OS tissues, as well as in U-2OS and MG-63 cells, when compared with normal tissues and osteoblasts, respectively. Our data further indicated that MACC1 expression was correlated with several clinicopathological features of OS. Through in vitro assays, we found that MACC1 depletion markedly suppressed the proliferative ability of both OS cells and endothelial cells, and inhibited the angiogenic capacity of endothelial cells. Similarly, MACC1 depletion inhibited tumor growth, metastasis, and angiogenesis in mice. Mechanistically, we found that MACC1 could bind to the MET promoter, and enhanced the proliferation of both OS cells and endothelial cells through the HGF/c-Met signaling pathway. Furthermore, we show that MACC1 also promoted angiogenesis by regulating microtubule dynamics, thereby promoting the progression of OS. Our results indicate that MACC1 may be a new and promising therapeutic target for the treatment of OS.

APC2 plays an essential role in the axonal projection through the regulation of microtubule stability

2009 ◽  
Vol 65 ◽  
pp. S95
Author(s):  
Takafumi Shintani ◽  
Masaru Ihra ◽  
Sachiko Tani ◽  
Juichi Sakuraba ◽  
Hiraki Sakuta ◽  
...  
Keyword(s):  
Essential Role ◽  
Axonal Projection ◽  
Microtubule Stability
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