Filament-guided filament assembly provides structural memory of filament alignment during cytokinesis

2021 ◽  
Author(s):  
Younan Li ◽  
Edwin Munro
Keyword(s):  
Filament Assembly ◽  
Structural Memory

scholarly journals Existing actin filaments orient new filament growth to provide structural memory of filament alignment during cytokinesis

2020 ◽  
Author(s):  
Younan Li ◽  
Edwin Munro
Keyword(s):  
Single Molecule ◽  
Actin Filaments ◽  
Animal Cells ◽  
Effective Lifetime ◽  
C Elegans ◽  
Ring Assembly ◽  
Filament Assembly ◽  
Aligned Array ◽  
Single Molecule Analysis ◽  
Structural Memory

AbstractDuring cytokinesis, animal cells rapidly remodel the equatorial cortex to build an aligned array of actin filaments called the contractile ring. Local reorientation of filaments by equatorial contraction is thought to underlie the emergence of filament alignment during ring assembly. Here, combining single molecule analysis and modeling in one-cell C. elegans embryos, we show that filaments turnover is far too fast for reorientation of single filaments by equatorial contraction/cortex compression to explain the observed alignment, even if favorably oriented filaments are selectively stabilized. Instead, by tracking single Formin/CYK-1::GFP speckles to monitor local filament assembly, we identify a mechanism that we call filament-guided filament assembly (FGFA), in which existing filaments serve as templates to guide/orient the growth of new filaments. We show that FGFA sharply increases the effective lifetime of filament orientation, providing structural memory that allows slow equatorial contraction to build and maintain highly aligned filament arrays, despite rapid turnover of individual filaments.

Verification of hypothesis about structural memory of solutions of polymorphic modifications using NMR spectroscopy

2021 ◽  
Vol 70 (1) ◽  
pp. 91-98
Author(s):  
N. E. Kuz’mina ◽  
S. V. Moiseev ◽  
V. S. Kuz’min ◽  
M. D. Khorol’skiy ◽  
A. I. Luttseva
Keyword(s):  
Nmr Spectroscopy ◽  
Polymorphic Modifications ◽  
Structural Memory

scholarly journals Polylysine induces an antiparallel actin dimer that nucleates filament assembly. Crystal structure at 3.5-Å resolution.

2002 ◽  
Vol 277 (36) ◽  
pp. 33529
Author(s):  
Michael R. Bubb ◽  
Lakshmanan Govindasamy ◽  
Elena G. Yarmola ◽  
Sergey M. Vorobiev ◽  
Steven C. Almo ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Filament Assembly

scholarly journals Human keratin 1/10‐1B tetramer structures reveal a knob‐pocket mechanism in intermediate filament assembly

2019 ◽  
Vol 38 (11) ◽  
Author(s):  
Sherif A Eldirany ◽  
Minh Ho ◽  
Alexander J Hinbest ◽  
Ivan B Lomakin ◽  
Christopher G Bunick
Keyword(s):  
Intermediate Filament ◽  
Filament Assembly ◽  
Keratin 1
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