scholarly journals Transit-Amplifying Cells Coordinate Changes in Intestinal Epithelial Cell-Type Composition

2021 ◽  
Vol 56 (3) ◽  
pp. 356-365.e9
Author(s):  
Laura E. Sanman ◽  
Ina W. Chen ◽  
Jake M. Bieber ◽  
Veronica Steri ◽  
Coralie Trentesaux ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Intestinal Epithelial Cell ◽  
Intestinal Epithelial ◽  
Cell Type ◽  
Epithelial Cell Type ◽  
Cell Type Composition ◽  
Type Composition ◽  
Coordinate Changes

scholarly journals Phenotypic shift in human differentiated gastric cancers from gastric to intestinal epithelial cell type during disease progression

1998 ◽  
Vol 1 (2) ◽  
pp. 134-141 ◽  
Author(s):  
Akemi Yoshikawa ◽  
Ken-ichi Inada ◽  
Takasuke Yamachika ◽  
Nobuyuki Shimizu ◽  
Michio Kaminishi ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Disease Progression ◽  
Intestinal Epithelial Cell ◽  
Intestinal Epithelial ◽  
Cell Type ◽  
Epithelial Cell Type ◽  
Gastric Cancers ◽  
Phenotypic Shift

scholarly journals Transit-amplifying cells coordinate changes in intestinal epithelial cell-type composition

2019 ◽  
Author(s):  
Laura E. Sanman ◽  
Ina W. Chen ◽  
Jake M. Bieber ◽  
Veronica Steri ◽  
Byron Hann ◽  
...  
Keyword(s):  
Quantitative Imaging ◽  
Cell Types ◽  
Culture Conditions ◽  
Tissue Cell ◽  
Specific Cell ◽  
Cell Type ◽  
Cell Type Composition ◽  
Type Composition ◽  
Coordinate Changes

AbstractRenewing tissues have the remarkable ability to continually produce both proliferative progenitor and specialized differentiated cell-types. How are complex milieus of microenvironmental signals interpreted to coordinate tissue cell-type composition? Here, we develop a high-throughput approach that combines organoid technology and quantitative imaging to address this question in the context of the intestinal epithelium. Using this approach, we comprehensively survey enteroid responses to individual and paired perturbations to eight epithelial signaling pathways. We uncover culture conditions that enrich for specific cell-types, including Lgr5+ stem and enteroendocrine cells. We analyze interactions between perturbations and dissect mechanisms underlying an unexpected mutual antagonism between EGFR and IL-4 signals. Finally, we show that, across diverse perturbations, modulating proliferation of transit-amplifying cells also consistently changes the composition of differentiated secretory and absorptive cell-types. This property is conserved in vivo and can arise from differential amplification of secretory and absorptive progenitor cells. Taken together, the observations highlight an underappreciated role for transit-amplifying cells in which proliferation of these short-lived progenitors provides a lineage-based mechanism for tuning differentiated cell-type composition.

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