scholarly journals Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling Couples Proliferation and Cell Death Mechanisms

2019 ◽  
Vol 51 (2) ◽  
pp. 277-291.e4 ◽  
Author(s):  
Jesse D. Gelles ◽  
Jarvier N. Mohammed ◽  
Luis C. Santos ◽  
Diana Legarda ◽  
Adrian T. Ting ◽  
...  
Keyword(s):  
Cell Death ◽  
Single Cell ◽  
Real Time ◽  
Population Level ◽  
Cell Death Mechanisms

scholarly journals Real-time integration of cell death mechanisms and proliferation kinetics at the single-cell and population-level using high-throughput live-cell imaging

2019 ◽  
Author(s):  
Jesse D. Gelles ◽  
Jarvier N. Mohammed ◽  
Luis C. Santos ◽  
Diana Legarda ◽  
Adrian T. Ting ◽  
...  
Keyword(s):  
Cell Death ◽  
Single Cell ◽  
Real Time ◽  
High Throughput ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Time Integration ◽  
Population Level ◽  
Live Cell ◽  
Cell Death Mechanisms

SummaryQuantifying cytostatic and cytotoxic outcomes are integral components of characterizing perturbagens used as research tools and/or in drug discovery pipelines. Furthermore, data-rich acquisition coupled with robust methods for analysis is required to properly assess the function and impact of these perturbagens. Here, we present a detailed and versatile method for Single-cell and Population-level Analyses using Real-time Kinetic Labeling (SPARKL). SPARKL integrates high-content live-cell imaging with automated detection and analysis of fluorescent reporters of cell death. We outline several examples of zero-handling, non-disruptive protocols for detailing cell death mechanisms and proliferation profiles. Additionally, we suggest several methods for mathematically analyzing these data to best utilize the collected kinetic data. Compared to traditional methods of detection and analysis, SPARKL is more sensitive, accurate, and high-throughput while substantially eliminating sample processing and providing richer data.

scholarly journals Single-cell mutagenic responses and cell death revealed in real time

2018 ◽  
Vol 115 (28) ◽  
pp. 7168-7170 ◽  
Author(s):  
Bennett Van Houten ◽  
Neil M. Kad
Keyword(s):  
Cell Death ◽  
Single Cell ◽  
Real Time

Different cell death mechanisms are triggered by acute or chronic right heart failure-induced liver failure

2011 ◽  
Vol 49 (01) ◽  
Author(s):  
K Herzer ◽  
G Kneiseler ◽  
F Post ◽  
M Schlattjan ◽  
T Neumann ◽  
...  
Keyword(s):  
Heart Failure ◽  
Cell Death ◽  
Liver Failure ◽  
Right Heart Failure ◽  
Right Heart ◽  
Cell Death Mechanisms

EFFECT OF COLCHICINE AND ATORVASTATIN ON CELL DEATH MECHANISMS DURING INFLAMMATORY PROCESSES IN ATHEROSCHLEROSIS

2018 ◽  
Vol 74 (11) ◽  
Author(s):  
Gunnur Demircan ◽  
Sule Beyhan Ozdas ◽  
Demet Akin ◽  
Ozgur Kaplan ◽  
Sabri Demircan ◽  
...  
Keyword(s):  
Cell Death ◽  
Inflammatory Processes ◽  
Cell Death Mechanisms

Cytotoxicity and Cell Death Mechanisms Induced by a Novel Bisnaphthalimidopropyl Derivative against the NCI-H460 non-small Lung Cancer Cell Line

2013 ◽  
Vol 13 (3) ◽  
pp. 414-421 ◽  
Author(s):  
Raquel T. Lima ◽  
Gemma A. Barron ◽  
Joanna A. Grabowska ◽  
Giovanna Bermano ◽  
Simranjeet Kaur ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Death ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Lung Cancer Cell Line ◽  
Lung Cancer Cell ◽  
Cell Death Mechanisms

Organelle Stress Sensors and Cell Death Mechanisms in Neurodegenerative Diseases

2010 ◽  
Vol 9 (6) ◽  
pp. 679-692 ◽  
Author(s):  
Ricardo J.S. Viana ◽  
Maria B. Fonseca ◽  
Rita M. Ramalho ◽  
Ana F. Nunes ◽  
Cecilia M.P. Rodrigues
Keyword(s):  
Cell Death ◽  
Neurodegenerative Diseases ◽  
Stress Sensors ◽  
Cell Death Mechanisms

scholarly journals Suppressing Pyroptosis Augments Post-Transplant Survival of Stem Cells and Cardiac Function Following Ischemic Injury

2021 ◽  
Vol 22 (15) ◽  
pp. 7946
Author(s):  
Chang Youn Lee ◽  
Seahyoung Lee ◽  
Seongtae Jeong ◽  
Jiyun Lee ◽  
Hyang-Hee Seo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Death ◽  
Stem Cell ◽  
Cardiac Fibrosis ◽  
Ischemia Reperfusion ◽  
Cardiac Cells ◽  
Transplant Survival ◽  
Post Transplant ◽  
Heart Functions ◽  
Cell Death Mechanisms

The acute demise of stem cells following transplantation significantly compromises the efficacy of stem cell-based cell therapeutics for infarcted hearts. As the stem cells transplanted into the damaged heart are readily exposed to the hostile environment, it can be assumed that the acute death of the transplanted stem cells is also inflicted by the same environmental cues that caused massive death of the host cardiac cells. Pyroptosis, a highly inflammatory form of programmed cell death, has been added to the list of important cell death mechanisms in the damaged heart. However, unlike the well-established cell death mechanisms such as necrosis or apoptosis, the exact role and significance of pyroptosis in the acute death of transplanted stem cells have not been explored in depth. In the present study, we found that M1 macrophages mediate the pyroptosis in the ischemia/reperfusion (I/R) injured hearts and identified miRNA-762 as an important regulator of interleukin 1b production and subsequent pyroptosis. Delivery of exogenous miRNA-762 prior to transplantation significantly increased the post-transplant survival of stem cells and also significantly ameliorated cardiac fibrosis and heart functions following I/R injury. Our data strongly suggest that suppressing pyroptosis can be an effective adjuvant strategy to enhance the efficacy of stem cell-based therapeutics for diseased hearts.

scholarly journals Multi-Modal Multi-Spectral Intravital Microscopic Imaging of Signaling Dynamics in Real-Time during Tumor–Immune Interactions

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 499
Author(s):  
Tracy W. Liu ◽  
Seth T. Gammon ◽  
David Piwnica-Worms
Keyword(s):  
Molecular Imaging ◽  
Single Cell ◽  
Real Time ◽  
Bioluminescence Imaging ◽  
Emission Spectra ◽  
Ccd Camera ◽  
Microscopic Imaging ◽  
Signaling Dynamics ◽  
Window Chamber

Intravital microscopic imaging (IVM) allows for the study of interactions between immune cells and tumor cells in a dynamic, physiologically relevant system in vivo. Current IVM strategies primarily use fluorescence imaging; however, with the advances in bioluminescence imaging and the development of new bioluminescent reporters with expanded emission spectra, the applications for bioluminescence are extending to single cell imaging. Herein, we describe a molecular imaging window chamber platform that uniquely combines both bioluminescent and fluorescent genetically encoded reporters, as well as exogenous reporters, providing a powerful multi-plex strategy to study molecular and cellular processes in real-time in intact living systems at single cell resolution all in one system. We demonstrate that our molecular imaging window chamber platform is capable of imaging signaling dynamics in real-time at cellular resolution during tumor progression. Importantly, we expand the utility of IVM by modifying an off-the-shelf commercial system with the addition of bioluminescence imaging achieved by the addition of a CCD camera and demonstrate high quality imaging within the reaches of any biology laboratory.

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