scholarly journals The Vertebrate Protein Dead End Maintains Primordial Germ Cell Fate by Inhibiting Somatic Differentiation

2017 ◽  
Vol 43 (6) ◽  
pp. 704-715.e5 ◽  
Author(s):  
Theresa Gross-Thebing ◽  
Sargon Yigit ◽  
Jana Pfeiffer ◽  
Michal Reichman-Fried ◽  
Jan Bandemer ◽  
...  
Keyword(s):  
Germ Cell ◽  
Cell Fate ◽  
Primordial Germ Cell ◽  
Dead End ◽  
Vertebrate Protein

scholarly journals A critical role of PRDM14 in human primordial germ cell fate revealed by inducible degrons

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Anastasiya Sybirna ◽  
Walfred W. C. Tang ◽  
Merrick Pierson Smela ◽  
Sabine Dietmann ◽  
Wolfram H. Gruhn ◽  
...  
Keyword(s):  
Germ Cell ◽  
Cell Fate ◽  
Critical Role ◽  
Primordial Germ Cell

scholarly journals Single Cell Sequencing Reveals Early PGC-like Intermediates During Mouse Primed to Naïve Transition

2021 ◽  
Author(s):  
Jing Liu ◽  
Shengyong Yu ◽  
Chunhua Zhou ◽  
Jiangping He ◽  
Xingnan Huang ◽  
...  
Keyword(s):  
Germ Cell ◽  
Single Cell ◽  
Cell Fate ◽  
Single Cell Analysis ◽  
Primordial Germ Cell ◽  
Model System ◽  
Cell Analysis ◽  
Rna Seq ◽  
Single Cell Sequencing

Abstract Single cell analysis provides clarity unattainable with bulk approaches. Here we apply single cell RNA-seq to a newly established BMP4 induced mouse primed to naive transition (Bi-PNT) system and show that the reset is not a direct reversal of cell fate but through developmental intermediates. We first show that mEpiSCs bifurcate into c-Kit+ naïve and c-Kit- placenta-like cells, among which, the naive branch undergoes further transition through a primordial germ cell-like cells (PGCLCs) intermediate capable of spermatogenesis in vivo. Indeed, deficiency of Prdm1/Blimp1, the key regulator for PGC specification, blocks the induction of PGCLCs and naïve cells. Instead, Gata2 knockout arrests placenta-like fate, but facilitates the generation of PGCLCs. Our results not only reveal a newly cell fate dynamics between primed and naive states at single-cell resolution, but also provide a model system to explore mechanisms involved in regaining germline competence from primed pluripotency.

scholarly journals Genetic dissection of Nodal and Bmp signalling requirements during primordial germ cell development

2018 ◽  
Author(s):  
Anna D. Senft ◽  
Elizabeth K. Bikoff ◽  
Elizabeth J. Robertson ◽  
Ita Costello
Keyword(s):  
Germ Cell ◽  
Cell Fate ◽  
Primordial Germ Cell ◽  
Genetic Dissection ◽  
Mouse Development ◽  
Cell Fate Decisions ◽  
Conditional Deletion ◽  
Bmp Signalling ◽  
And Migration ◽  
Early Mouse

AbstractThe essential roles played by Nodal and Bmp signalling during early mouse development have been extensively documented. Here we used conditional deletion strategies to investigate functional contributions made by Nodal, Bmp and Smad downstream effectors during primordial germ cell (PGC) development. We demonstrate that Nodal and its target gene Eomes provide early instructions during formation of the PGC lineage. We discovered that Smad2 inactivation in the visceral endoderm results in increased numbers of PGCs due to an expansion of the PGC niche. Smad1 is required for specification, whereas in contrast Smad4 controls the maintenance and migration of PGCs. Importantly we found that beside Blimp1, down-regulated phosphoSmad159 levels also distinguishes PGCs from their somatic neighbours so that emerging PGCs become refractory to Bmp signalling that otherwise promotes mesodermal development in the posterior epiblast. Thus balanced Nodal/Bmp signalling cues regulate germ cell versus somatic cell fate decisions in the early posterior epiblast.

scholarly journals Extensive nuclear gyration and pervasive non-genic transcription during primordial germ cell development in zebrafish

Development ◽  
2020 ◽  
pp. dev.193060
Author(s):  
Stefan Redl ◽  
Antonio M. de Jesus Domingues ◽  
Edoardo Caspani ◽  
Stefanie Möckel ◽  
Willi Salvenmoser ◽  
...  
Keyword(s):  
Germ Cell ◽  
Cell Fate ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Primordial Germ Cell ◽  
Genital Ridge ◽  
Non Coding Rna ◽  
Pathway Activation ◽  
Pirna Pathway ◽  
Time Point

Primordial germ cells (PGCs) are the precursors of germ cells, which migrate to the genital ridge during early development. Relatively little is known about PGCs after their migration. We studied this post-migratory stage using microscopy and sequencing techniques, and found that many PGC-specific genes, including genes known to induce PGC fate in the mouse, are only activated several days after migration. At this same time point, PGC nuclei become extremely gyrated, displaying general broad opening of chromatin and high levels of intergenic transcription. This is accompanied by changes in nuage morphology, expression of large loci (PGC-Expressed non-coding RNA Loci, PERLs) that are enriched for retro-transposons and piRNAs, and a rise in piRNA biogenesis signatures. Interestingly, no nuclear Piwi protein could be detected at any time point, indicating that the zebrafish piRNA pathway is fully cytoplasmic. Our data show that the post-migratory stage of zebrafish PGCs holds many cues to both germ cell fate establishment and piRNA pathway activation.

scholarly journals dead end, a Novel Vertebrate Germ Plasm Component, Is Required for Zebrafish Primordial Germ Cell Migration and Survival

2003 ◽  
Vol 13 (16) ◽  
pp. 1429-1434 ◽  
Author(s):  
Gilbert Weidinger ◽  
Jürg Stebler ◽  
Krasimir Slanchev ◽  
Karin Dumstrei ◽  
Clare Wise ◽  
...  
Keyword(s):  
Cell Migration ◽  
Germ Cell ◽  
Germ Plasm ◽  
Primordial Germ Cell ◽  
Dead End ◽  
Germ Cell Migration

scholarly journals SETDB1 is essential for mouse primordial germ cell fate determination by ensuring BMP signaling

Development ◽  
2018 ◽  
Vol 145 (23) ◽  
pp. dev164160 ◽  
Author(s):  
Kentaro Mochizuki ◽  
Yukiko Tando ◽  
Tamotsu Sekinaka ◽  
Kei Otsuka ◽  
Yohei Hayashi ◽  
...  
Keyword(s):  
Germ Cell ◽  
Cell Fate ◽  
Primordial Germ Cell ◽  
Bmp Signaling ◽  
Cell Fate Determination ◽  
Fate Determination ◽  
Mouse Primordial Germ Cell
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