scholarly journals Distinct Requirements for FGFR1 and FGFR2 in Primitive Endoderm Development and Exit from Pluripotency

2017 ◽  
Vol 41 (5) ◽  
pp. 511-526.e4 ◽  
Author(s):  
Andrei Molotkov ◽  
Pierre Mazot ◽  
J. Richard Brewer ◽  
Ryan M. Cinalli ◽  
Philippe Soriano
Keyword(s):  
Primitive Endoderm ◽  
Endoderm Development

scholarly journals Interleukin-6 promotes primitive endoderm development in bovine blastocysts

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Lydia K. Wooldridge ◽  
Alan D. Ealy
Keyword(s):  
Interleukin 6 ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Cell Types ◽  
Janus Kinase ◽  
Primitive Endoderm ◽  
Cell Numbers ◽  
Downstream Target ◽  
Dependent Signal ◽  
Endoderm Development

Abstract Background Interleukin-6 (IL6) was recently identified as an embryotrophic factor in bovine embryos, where it acts primarily to mediate inner cell mass (ICM) size. This work explored whether IL6 affects epiblast (EPI) and primitive endoderm (PE) development, the two embryonic lineages generated from the ICM after its formation. Nuclear markers for EPI (NANOG) and PE (GATA6) were used to differentiate the two cell types. Results Increases (P < 0.05) in total ICM cell numbers and PE cell numbers were detected in bovine blastocysts at day 8 and 9 post-fertilization after exposure to 100 ng/ml recombinant bovine IL6. Also, IL6 increased (P < 0.05) the number of undifferentiated ICM cells (cells containing both PE and EPI markers). The effects of IL6 on EPI cell numbers were inconsistent. Studies were also completed to explore the importance of Janus kinase 2 (JAK2)-dependent signaling in bovine PE cells. Definitive activation of STAT3, a downstream target for JAK2, was observed in PE cells. Also, pharmacological inhibition of JAK2 decreased (P < 0.05) PE cell numbers. Conclusions To conclude, IL6 manipulates ICM development after EPI/PE cell fates are established. The PE cells are the target for IL6, where a JAK-dependent signal is used to regulate PE numbers.

scholarly journals Gutsy moves in mice: cellular and molecular dynamics of endoderm morphogenesis

2014 ◽  
Vol 369 (1657) ◽  
pp. 20130547 ◽  
Author(s):  
Manuel Viotti ◽  
Ann C. Foley ◽  
Anna-Katerina Hadjantonakis
Keyword(s):  
Molecular Dynamics ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Complex Cell ◽  
Primitive Endoderm ◽  
Imaging Technologies ◽  
Cell Movements ◽  
Endoderm Development ◽  
Endodermal Layer

Despite the importance of the gut and its accessory organs, our understanding of early endoderm development is still incomplete. Traditionally, endoderm has been difficult to study because of its small size and relative fragility. However, recent advances in live cell imaging technologies have dramatically expanded our understanding of this tissue, adding a new appreciation for the complex molecular and morphogenetic processes that mediate gut formation. Several spatially and molecularly distinct subpopulations have been shown to exist within the endoderm before the onset of gastrulation. Here, we review findings that have uncovered complex cell movements within the endodermal layer, before and during gastrulation, leading to the conclusion that cells from primitive endoderm contribute descendants directly to gut.

FGF2 Promotes Primitive Endoderm Development in Bovine Blastocysts.

2010 ◽  
Vol 83 (Suppl_1) ◽  
pp. 181-181
Author(s):  
Qi-En Yang ◽  
Sarah D. Fields ◽  
Kun Zhang ◽  
Manabu Ozawa ◽  
Sally E. Johnson ◽  
...  
Keyword(s):  
Primitive Endoderm ◽  
Endoderm Development

scholarly journals Interleukin-6 Promotes Primitive Endoderm Development in Bovine Blastocysts

2020 ◽  
Author(s):  
Lydia K. Wooldridge ◽  
Alan D. Ealy
Keyword(s):  
Interleukin 6 ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Cell Types ◽  
Janus Kinase ◽  
Primitive Endoderm ◽  
Cell Numbers ◽  
Downstream Target ◽  
Dependent Signal ◽  
Endoderm Development

Abstract Background: Interleukin-6 (IL6) was recently identified as an embryotrophic factor in bovine embryos, where it acts primarily to mediate inner cell mass (ICM) size. This work explored whether IL6 affects epiblast (EPI) and primitive endoderm (PE) development, the two embryonic lineages generated from the ICM after its formation. Nuclear markers for EPI (NANOG) and PE (GATA6) were used to differentiate the two cell types. Results: Increases (P<0.05) in total ICM cell numbers and PE cell numbers were detected in bovine blastocysts at day 8 and 9 post-fertilization after exposure to 100 ng/ml recombinant bovine IL6. Also, IL6 increased (P<0.05) the number of undifferentiated ICM cells (cells containing both PE and EPI markers). The effects of IL6 on EPI cell numbers were inconsistent. Studies were also completed to explore the importance of Janus kinase 2 (JAK2)-dependent signaling in bovine PE cells. Definitive activation of STAT3, a downstream target for JAK2, was observed in PE cells. Also, pharmacological inhibition of JAK2 decreased (P<0.05) PE cell numbers. Conclusions: To conclude, IL6 manipulates ICM development after EPI/PE cell fates are established. The PE cells are the target for IL6, where a JAK-dependent signal is used to regulate PE numbers.

scholarly journals Fibroblast Growth Factor 2 Promotes Primitive Endoderm Development in Bovine Blastocyst Outgrowths1

2011 ◽  
Vol 85 (5) ◽  
pp. 946-953 ◽  
Author(s):  
Qi En Yang ◽  
Sarah D. Fields ◽  
Kun Zhang ◽  
Manabu Ozawa ◽  
Sally E. Johnson ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Primitive Endoderm ◽  
Endoderm Development ◽  
Bovine Blastocyst

scholarly journals Oct4 Cell-Autonomously Promotes Primitive Endoderm Development in the Mouse Blastocyst

2013 ◽  
Vol 25 (6) ◽  
pp. 610-622 ◽  
Author(s):  
Tristan Frum ◽  
Michael A. Halbisen ◽  
Chaoyang Wang ◽  
Hossein Amiri ◽  
Paul Robson ◽  
...  
Keyword(s):  
Primitive Endoderm ◽  
Mouse Blastocyst ◽  
Endoderm Development

scholarly journals Interleukin-6 promotes primitive endoderm development in bovine blastocysts

2020 ◽  
Author(s):  
Lydia K. Wooldridge ◽  
Alan Ealy
Keyword(s):  
Interleukin 6 ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Cell Types ◽  
Janus Kinase ◽  
Primitive Endoderm ◽  
Cell Numbers ◽  
Downstream Target ◽  
Dependent Signal ◽  
Endoderm Development

Abstract Background: Interleukin-6 (IL6) was recently identified as an embryotrophic factor in bovine embryos, where it acts primarily to mediate inner cell mass (ICM) size. This work explored whether IL6 affects epiblast (EPI) and primitive endoderm (PE) development, the two embryonic lineages generated from the ICM after its formation. Nuclear markers for EPI (NANOG) and PE (GATA6) were used to differentiate the two cell types. Results: Increases (P<0.05) in total ICM cell numbers and PE cell numbers were detected in bovine blastocysts at day 8 and 9 post-fertilization after exposure to 100 ng/ml recombinant bovine IL6. Also, IL6 increased (P<0.05) the number of undifferentiated ICM cells (cells containing both PE and EPI markers). The effects of IL6 on EPI cell numbers were inconsistent. Studies were also completed to explore the importance of Janus kinase 2 (JAK2)-dependent signaling in bovine PE cells. Definitive activation of STAT3, a downstream target for JAK2, was observed in PE cells. Also, pharmacological inhibition of JAK2 decreased (P<0.05) PE cell numbers. Conclusions: To conclude, IL6 manipulates ICM development after EPI/PE cell fates are established. The PE cells are the target for IL6, where a JAK-dependent signal is used to regulate PE numbers.

scholarly journals Molecular regulation of primitive endoderm development

2009 ◽  
Vol 331 (2) ◽  
pp. 502
Author(s):  
Roy Teo ◽  
Samantha Morris ◽  
Magdalena Zernicka-Goetz ◽  
Paul Robson
Keyword(s):  
Molecular Regulation ◽  
Primitive Endoderm ◽  
Endoderm Development
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