A Rab10-Dependent Mechanism for Polarized Basement Membrane Secretion during Organ Morphogenesis

Two month, eight month and two year old rats were treated with 10 or 20 mg/kg of E. Coli endotoxin I. P. The eight month old rats proved most resistant to the endotoxin. During fixation the aorta, carotid artery, basil arartery of the brain, coronary vessels of the heart, inner surfaces of the heart chambers, heart and skeletal muscle, lung, liver, kidney, spleen, brain, retina, trachae, intestine, salivary gland, adrenal gland and gingiva were treated with ruthenium red or alcian blue to preserve the mucopolysaccharide (MPS) coating. Five, 8 and 24 hrs of endotoxin treatment produced increasingly marked capillary damage, disappearance of the MPS coating, edema, destruction of endothelial cells and damage to the basement membrane in the liver, kidney and lung.

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Physical Properties of Isolated Perfused Renal Tubular Basement Membranes

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100065997 ◽

1971 ◽

Vol 29 ◽

pp. 390-391

Author(s):

Jared Grantham ◽

Larry Welling

Keyword(s):

Basement Membrane ◽

Basement Membranes ◽

Transport Mechanisms ◽

Permeability Characteristics ◽

Peritubular Capillaries ◽

Strategic Location ◽

Tubular Lumen ◽

Glomerular Filtrate ◽

Urine Formation ◽

Renal Tubular

In the course of urine formation in mammalian kidneys over 90% of the glomerular filtrate moves from the tubular lumen into the peritubular capillaries by both active and passive transport mechanisms. In all of the morphologically distinct segments of the renal tubule, e.g. proximal tubule, loop of Henle and distal nephron, the tubular absorbate passes through a basement membrane which rests against the basilar surface of the epithelial cells. The basement membrane is in a strategic location to affect the geometry of the tubules and to influence the movement of tubular absorbate into the renal interstitium. In the present studies we have determined directly some of the mechanical and permeability characteristics of tubular basement membranes.

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Kalinin: A new epithelial basement membrane adhesion molecule

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100085198 ◽

1991 ◽

Vol 49 ◽

pp. 178-179

Author(s):

Douglas R. Keene ◽

Gregory P. Lunstrum ◽

Patricia Rousselle ◽

Robert E. Burgeson

Keyword(s):

Basement Membrane ◽

Culture Media ◽

Polyacrylamide Gel Electrophoresis ◽

Human Keratinocytes ◽

Positive Staining ◽

Human Amnion ◽

Epithelial Basement Membrane ◽

Type Vii Collagen ◽

Keratinocyte Cell ◽

Epithelial Basement

A mouse monoclonal antibody produced from collagenase digests of human amnion was used by LM and TEM to study the distribution and ultrastructural features of an antigen present in epithelial tissues and in cultured human keratinocytes, and by immunoaffinity chromatography to partially purify the antigen from keratinocyte cell culture media.By immunofluorescence microscopy, the antigen displays a tissue distribution similar to type VII collagen; positive staining of the epithelial basement membrane is seen in skin, oral mucosa, trachea, esophagus, cornea, amnion and lung. Images from rotary shadowed preparations isolated by affinity chromatography demonstrate a population of rod-like molecules 107 nm in length, having pronounced globular domains at each end. Polyacrylamide gel electrophoresis suggests that the size of this molecule is approximately 440kDa, and that it is composed of three nonidentical chains disulfide bonded together.

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