scholarly journals Perinuclear Cohibin Complexes Maintain Replicative Life Span via Roles at Distinct Silent Chromatin Domains

2011 ◽  
Vol 20 (6) ◽  
pp. 867-879 ◽  
Author(s):  
Janet N.Y. Chan ◽  
Betty P.K. Poon ◽  
Jayesh Salvi ◽  
Jonathan B. Olsen ◽  
Andrew Emili ◽  
...  
Keyword(s):  
Life Span ◽  
Silent Chromatin ◽  
Chromatin Domains ◽  
Replicative Life Span

Telomerase Alone Extends the Replicative Life Span of Human Skeletal Muscle Cells Without Compromising Genomic Stability

2003 ◽  
Vol 14 (15) ◽  
pp. 1473-1487 ◽  
Author(s):  
Martha Wootton ◽  
Karen Steeghs ◽  
Diana Watt ◽  
June Munro ◽  
Katrina Gordon ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Life Span ◽  
Human Skeletal Muscle ◽  
Genomic Stability ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Replicative Life Span ◽  
Human Skeletal Muscle Cells

scholarly journals Loss of Ubp3 increases silencing, decreases unequal recombination in rDNA, and shortens the replicative life span in Saccharomyces cerevisiae

2014 ◽  
Vol 25 (12) ◽  
pp. 1916-1924 ◽  
Author(s):  
David Öling ◽  
Rehan Masoom ◽  
Kristian Kvint
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Rna Polymerase ◽  
Life Span ◽  
Rna Polymerase Ii ◽  
Ribosomal Dna ◽  
Genetic Evidence ◽  
Wild Type ◽  
Replicative Life Span ◽  
Unequal Recombination

Ubp3 is a conserved ubiquitin protease that acts as an antisilencing factor in MAT and telomeric regions. Here we show that ubp3∆ mutants also display increased silencing in ribosomal DNA (rDNA). Consistent with this, RNA polymerase II occupancy is lower in cells lacking Ubp3 than in wild-type cells in all heterochromatic regions. Moreover, in a ubp3∆ mutant, unequal recombination in rDNA is highly suppressed. We present genetic evidence that this effect on rDNA recombination, but not silencing, is entirely dependent on the silencing factor Sir2. Further, ubp3∆ sir2∆ mutants age prematurely at the same rate as sir2∆ mutants. Thus our data suggest that recombination negatively influences replicative life span more so than silencing. However, in ubp3∆ mutants, recombination is not a prerequisite for aging, since cells lacking Ubp3 have a shorter life span than isogenic wild-type cells. We discuss the data in view of different models on how silencing and unequal recombination affect replicative life span and the role of Ubp3 in these processes.

scholarly journals Formation of Boundaries of Transcriptionally Silent Chromatin by Nucleosome-Excluding Structures

2004 ◽  
Vol 24 (5) ◽  
pp. 2118-2131 ◽  
Author(s):  
Xin Bi ◽  
Qun Yu ◽  
Joseph J. Sandmeier ◽  
Yanfei Zou
Keyword(s):  
Chromatin Structure ◽  
Barrier Function ◽  
Eukaryotic Genome ◽  
Silent Chromatin ◽  
Active Chromatin ◽  
Chromatin Domains ◽  
Distinct Gene ◽  
Lexa Protein ◽  
Nucleosome Formation

ABSTRACT The eukaryotic genome is divided into chromosomal domains of distinct gene activities. Transcriptionally silent chromatin tends to encroach upon active chromatin. Barrier elements that can block the spread of silent chromatin have been documented, but the mechanisms of their function are not resolved. We show that the prokaryotic LexA protein can function as a barrier to the propagation of transcriptionally silent chromatin in yeast. The barrier function of LexA correlates with its ability to disrupt local chromatin structure. In accord with this, (CCGNN) n and poly(dA-dT), both of which do not favor nucleosome formation, can also act as efficient boundaries of silent chromatin. Moreover, we show that a Rap1p-binding barrier element also disrupts chromatin structure. These results demonstrate that nucleosome exclusion is one of the mechanisms for the establishment of boundaries of silent chromatin domains.

scholarly journals Stochastic Variation in Telomere Shortening Rate Causes Heterogeneity of Human Fibroblast Replicative Life Span

2004 ◽  
Vol 279 (17) ◽  
pp. 17826-17833 ◽  
Author(s):  
Carmen Martin-Ruiz ◽  
Gabriele Saretzki ◽  
Joanne Petrie ◽  
Juliane Ladhoff ◽  
Jessie Jeyapalan ◽  
...  
Keyword(s):  
Life Span ◽  
Human Fibroblast ◽  
Stochastic Variation ◽  
Telomere Shortening ◽  
Replicative Life Span ◽  
Shortening Rate

A comparison of the proliferative and replicative life span kinetics of cell cultures derived from monozygotic twins

In Vitro ◽  
1981 ◽  
Vol 17 (1) ◽  
pp. 20-27 ◽  
Author(s):  
J. M. Ryan ◽  
D. G. Ostrow ◽  
Xandra O. Breakefield ◽  
Elliot S. Gershon ◽  
Leo Upchurch
Keyword(s):  
Life Span ◽  
Cell Cultures ◽  
Monozygotic Twins ◽  
Replicative Life Span ◽  
Kinetics Of

Higher DNA repair activity is related with longer replicative life span in mammalian embryonic fibroblast cells

2011 ◽  
Vol 12 (6) ◽  
pp. 565-579 ◽  
Author(s):  
Seong-Hoon Park ◽  
Hong-Jun Kang ◽  
Hyun-Seok Kim ◽  
Min-Ju Kim ◽  
Jee-In Heo ◽  
...  
Keyword(s):  
Dna Repair ◽  
Life Span ◽  
Fibroblast Cells ◽  
Replicative Life Span ◽  
Repair Activity ◽  
Embryonic Fibroblast

Chronologic and physiologic age affect replicative life-span of fibroblasts from diabetic, prediabetic, and normal donors

Science ◽  
1978 ◽  
Vol 199 (4330) ◽  
pp. 781-782 ◽  
Author(s):  
S Goldstein ◽  
E. Moerman ◽  
J. Soeldner ◽  
R. Gleason ◽  
D. Barnett
Keyword(s):  
Life Span ◽  
Replicative Life Span
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