scholarly journals Dendrite Reshaping of Adult Drosophila Sensory Neurons Requires Matrix Metalloproteinase-Mediated Modification of the Basement Membranes

2010 ◽  
Vol 18 (4) ◽  
pp. 621-632 ◽  
Author(s):  
Kei-ichiro Yasunaga ◽  
Takahiro Kanamori ◽  
Rei Morikawa ◽  
Emiko Suzuki ◽  
Kazuo Emoto
Keyword(s):  
Matrix Metalloproteinase ◽  
Sensory Neurons ◽  
Basement Membranes ◽  
Adult Drosophila

scholarly journals Dendrite regeneration of adult Drosophila sensory neurons diminishes with aging and is inhibited by epidermal-derived matrix metalloproteinase 2

2018 ◽  
Vol 32 (5-6) ◽  
pp. 402-414 ◽  
Author(s):  
Laura DeVault ◽  
Tun Li ◽  
Sarah Izabel ◽  
Katherine L. Thompson-Peer ◽  
Lily Yeh Jan ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Sensory Neurons ◽  
Matrix Metalloproteinase 2 ◽  
Adult Drosophila

scholarly journals Staphylococcal Superantigen-Like Protein 5 Inhibits Matrix Metalloproteinase 9 from Human Neutrophils

2010 ◽  
Vol 78 (7) ◽  
pp. 3298-3305 ◽  
Author(s):  
Saotomo Itoh ◽  
Eri Hamada ◽  
Go Kamoshida ◽  
Kana Takeshita ◽  
Teruaki Oku ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Polyacrylamide Gel Electrophoresis ◽  
Peptide Mass Fingerprinting ◽  
Matrix Metalloproteinase 9 ◽  
Basement Membranes ◽  
Human Neutrophils ◽  
Surface Plasmon Resonance Analysis ◽  
Major Band ◽  
Peptide Mass ◽  
Metalloproteinase 9

ABSTRACT Staphylococcal superantigen-like proteins (SSLs) constitute a family of exoproteins exhibiting structural similarities to superantigens and enterotoxins but no superantigenic activity. In this article, we present evidence that SSL5 specifically binds to matrix metalloproteinase 9 (MMP-9) and inhibits its enzymatic activity. When human neutrophil cell lysate was applied to recombinant His-tagged SSL5 conjugated to Sepharose, the bound fraction gave a major band of approximately 100 kDa in SDS-polyacrylamide gel electrophoresis. This protein was identified as the proform of MMP-9 (proMMP-9) by peptide mass fingerprinting analysis. The recombinant SSL5-Sepharose also bound to proMMP-9 secreted by interleukin 8 (IL-8)-stimulated neutrophils and HT1080 fibrosarcoma cells. Surface plasmon resonance analysis revealed that recombinant SSL5 bound to proMMP-9 with rather high affinity (dissociation constant [K D] = 1.9 nM). Recombinant SSL5 was found to effectively inhibit MMP-9-catalyzed hydrolysis of gelatin and a synthetic fluorogenic peptide in a noncompetitive manner (K i = 0.097 nM), as assessed by zymography and the fluorescence quenching method. Finally, the transmigration of neutrophils across Matrigel basement membranes in response to N-formyl-methionyl-leucyl-phenylalanine (FMLP) was suppressed by the presence of recombinant SSL5. We discuss possible roles that SSL5 may play in immune evasion of staphylococci by inhibiting MMP and interfering with leukocyte trafficking.

scholarly journals Sensory neurons of the Atonal lineage pioneer the formation of glomeruli within the adult Drosophila olfactory lobe

Development ◽  
2002 ◽  
Vol 129 (5) ◽  
pp. 1251-1260 ◽  
Author(s):  
Dhanisha Jhaveri ◽  
Veronica Rodrigues
Keyword(s):  
Sensory Neurons ◽  
Glial Cells ◽  
Crucial Role ◽  
Sensory Innervation ◽  
Projection Neurons ◽  
Olfactory Lobe ◽  
Adult Drosophila

The first centers for processing of odor information by animals lie in the olfactory lobe. Sensory neurons from the periphery synapse with interneurons in anatomically recognizable units, termed glomeruli, seen in both insects and vertebrates. The mechanisms that underlie the formation of functional maps of the odor-world in the glomeruli within the olfactory lobe remains unclear. We address the basis of sensory targeting in the fruitfly Drosophila and show that one class of sensory neurons, those of the Atonal lineage, plays a crucial role in glomerular patterning. Atonal-dependent neurons pioneer the segregation of other classes of sensory neurons into distinct glomeruli. Furthermore, correct sensory innervation is necessary for the arborization of projection neurons into glomeruli and for the elaboration of processes of central glial cells into the lobe.

scholarly journals Level of serum matrix metalloproteinase-9 in patients with acute coronary syndrome in Vietnam

2018 ◽  
Vol 15 (1) ◽  
pp. 15-21
Author(s):  
Đỗ Hữu Chí ◽  
Nguyễn Thị Minh Phương ◽  
Bùi Thị Huyền ◽  
Nguyễn Tiến Dũng ◽  
Phạm Đình Minh ◽  
...  
Keyword(s):  
Acute Coronary Syndrome ◽  
Logistic Regression ◽  
Regression Analysis ◽  
Matrix Metalloproteinase ◽  
Logistic Regression Analysis ◽  
Basement Membranes ◽  
Atherosclerotic Plaques ◽  
St Segment Elevation ◽  
Coronary Syndrome ◽  
St Segment

Acute Coronary Syndrome (ACS) is one of the foremost causes of mortality and morbidity worldwide as well as Vietnam. ACS was evoked by rupture or erosion of atherosclerotic plaques that was divided into Non–ST-segment elevation Acute Coronary Syndrome (NSTEACS) and ST-segment elevation myocardial infarction groups (STEMI). The matrix metalloproteinase (MMPs) is an enzyme family with fucntioning in the degradation of extracellular matrix and disruption of basement membranes. Of the MMPs, MMP-9 is expressed in the atherosclerotic plaques and plays a key role in the rupture of vulnerable atherosclerotic plaques. In this work, we measured the serum MMP-9 level in 205 patients with acute coronary syndrome (including 103 of NSTE-ACS and 102 of STEMI patients) and 101 healthy participants by sandwich Elisa method. The results showed that the MMP-9 level was significantly higher in NSTE-ACS and STEMI compared to the control group (208.59 ± 100.47 ng/mL, 189.98 ± 112.81 ng/mL vs 168.50 ± 79.52 ng/mL, respectively, P=0.014). However, a logistic regression analysis indicated that the MMP-9 level was only significantly higher in patients with NSTE ACS (OR= 1.0048, CI 95%= 1.000-1.009; P= 0.018). The serum MMP-9 level is correlated with traditional risk factors such as glucose, Cholesterol and Triglyceride by multiple logistic regression analysis. Conclusion: Our study suggests that the serum MMP-9 level is significantly associated with the NSTEACS and is a potential marker for dianogsis of ACS in the Vietnamese patients

Alveolar epithelial cells in vitro produce gelatinases and tissue inhibitor of matrix metalloproteinase-2

1997 ◽  
Vol 273 (3) ◽  
pp. L663-L675 ◽  
Author(s):  
M. P. d'Ortho ◽  
C. Clerici ◽  
P. M. Yao ◽  
C. Delacourt ◽  
C. Delclaux ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Matrix Metalloproteinase ◽  
Primary Cultures ◽  
Basement Membranes ◽  
Alveolar Epithelial Cells ◽  
Matrix Metalloproteinase 2 ◽  
Type Ii ◽  
Matrix Metalloproteinase 1 ◽  
Type Ii Pneumocytes

Type II pneumocytes are key cells of the alveolar epithelium. They lie on the alveolar basement membrane, which influences their phenotype and functions. We hypothesized that type II pneumocytes degrade basement membrane components by producing gelatinases, members of the matrix metalloproteinase family. To investigate this hypothesis, we used primary cultures of rat type II pneumocytes and cultures of the human A549 cell line. We found by zymography that 70-kDa gelatinase was present in media conditioned by these cells. This 70-kDa gelatinase was identified as gelatinase A by a Western blot, and the presence of its mRNA was demonstrated by reverse transcription-polymerase chain reaction. A 95-kDa gelatinase could be induced under certain conditions. Production of gelatinases may take place during the turnover of basement membranes, in physiological and in pathophysiological processes. This was suggested by the increase in production of both gelatinases that we observed after in vitro exposure to LPS or interleukin-1. The presence of tissue inhibitors of matrix metalloproteinase-1 and -2 was also demonstrated, suggesting that degradation of extracellular matrix by type II pneumocytes is tightly regulated.

scholarly journals Olfactory neuron turnover in adult Drosophila

2020 ◽  
Author(s):  
Ismael Fernández-Hernández ◽  
Eric Hu ◽  
Michael A. Bonaguidi
Keyword(s):  
Sensory Neurons ◽  
Olfactory Epithelium ◽  
Olfactory System ◽  
Lineage Tracing ◽  
Olfactory Neuron ◽  
Neuronal Regeneration ◽  
Continuous Generation ◽  
The Brain ◽  
Adult Drosophila ◽  
Tracing Method

ABSTRACTSustained neurogenesis occurs in the olfactory epithelium of several species, including humans, to support olfactory function throughout life. We recently developed a modified lineage tracing method to identify adult neurogenesis in Drosophila. By applying this technique, here we report on the continuous generation of Olfactory Sensory Neurons (OSN) in the antennae of adult Drosophila. New neurons develop sensory dendrites and project axons targeting diverse glomeruli of the antennal lobes in the brain. Furthermore, we identified sustained apoptosis of OSN in the antennae of adult flies, revealing unexpected turnover in the adult olfactory system. Our results substantiate Drosophila as a compelling platform to expedite research about mechanisms and compounds promoting neuronal regeneration, circuit remodeling and its contribution to behavior in the adult.

Requirement of matrix metalloproteinase-1 for intestinal homeostasis in the adult Drosophila midgut

2012 ◽  
Vol 318 (5) ◽  
pp. 670-681 ◽  
Author(s):  
Shin-Hae Lee ◽  
Joung-Sun Park ◽  
Young-Shin Kim ◽  
Hae-Young Chung ◽  
Mi-Ae Yoo
Keyword(s):  
Matrix Metalloproteinase ◽  
Intestinal Homeostasis ◽  
Matrix Metalloproteinase 1 ◽  
Adult Drosophila

scholarly journals Drosophila gustatory projections are segregated by taste modality and connectivity

2021 ◽  
Author(s):  
Stefanie Engert ◽  
Gabriella R Sterne ◽  
David T Harris ◽  
Kristin Scott
Keyword(s):  
Electron Microscopy ◽  
Sensory Neurons ◽  
Right Hemisphere ◽  
Synaptic Connectivity ◽  
Synaptic Connections ◽  
The Right ◽  
Gustatory Neurons ◽  
Potential Food ◽  
The Brain ◽  
Adult Drosophila

Gustatory sensory neurons detect caloric and harmful compounds in potential food and convey this information to the brain to inform feeding decisions. To examine the signals that gustatory neurons transmit and receive, we reconstructed gustatory axons and their synaptic sites in the adult Drosophila melanogaster brain, utilizing a whole-brain electron microscopy volume. We reconstructed 87 gustatory projections from the proboscis labellum in the right hemisphere and 57 in the left, representing the majority of labellar gustatory axons. Morphology- and connectivity-based clustering revealed six distinct clusters, likely representing neurons recognizing different taste modalities. Gustatory neurons contain a nearly equal number of interspersed pre-and post-synaptic sites, with extensive synaptic connectivity among gustatory axons. The vast majority of synaptic connections are between morphologically similar neurons, although connections also exist between distinct neuronal subpopulations. This study resolves the anatomy of labellar gustatory projections, reveals that gustatory projections are likely segregated based on taste modality, and uncovers synaptic connections that may alter the transmission of gustatory signals.

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