scholarly journals The let-7 MicroRNA Family Members mir-48, mir-84, and mir-241 Function Together to Regulate Developmental Timing in Caenorhabditis elegans

2005 ◽  
Vol 9 (3) ◽  
pp. 403-414 ◽  
Author(s):  
Allison L. Abbott ◽  
Ezequiel Alvarez-Saavedra ◽  
Eric A. Miska ◽  
Nelson C. Lau ◽  
David P. Bartel ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Family Members ◽  
Developmental Timing ◽  
Microrna Family

scholarly journals The let-7 MicroRNA Family Members mir-48, mir-84, and mir-241 Function Together to Regulate Developmental Timing in Caenorhabditis elegans

2006 ◽  
Vol 10 (2) ◽  
pp. 271 ◽  
Author(s):  
Allison L. Abbott ◽  
Ezequiel Alvarez-Saavedra ◽  
Eric A. Miska ◽  
Nelson C. Lau ◽  
David P. Bartel ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Family Members ◽  
Developmental Timing ◽  
Microrna Family

Innexin-3 forms connexin-like intercellular channels

1999 ◽  
Vol 112 (14) ◽  
pp. 2391-2396 ◽  
Author(s):  
Y. Landesman ◽  
T.W. White ◽  
T.A. Starich ◽  
J.E. Shaw ◽  
D.A. Goodenough ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Gap Junction ◽  
Functional Properties ◽  
Xenopus Oocytes ◽  
Family Members ◽  
Electrical Coupling ◽  
Large Family ◽  
Gap Junction Channel ◽  
Intercellular Channels

Innexins comprise a large family of genes that are believed to encode invertebrate gap junction channel-forming proteins. However, only two Drosophila innexins have been directly tested for the ability to form intercellular channels and only one of those was active. Here we tested the ability of Caenorhabditis elegans family members INX-3 and EAT-5 to form intercellular channels between paired Xenopus oocytes. We show that expression of INX-3 but not EAT-5, induces electrical coupling between the oocyte pairs. In addition, analysis of INX-3 voltage and pH gating reveals a striking degree of conservation in the functional properties of connexin and innnexin channels. These data strongly support the idea that innexin genes encode intercellular channels.

scholarly journals Analysis of a lin-42/period Null Allele Implicates All Three Isoforms in Regulation of Caenorhabditis elegans Molting and Developmental Timing

2016 ◽  
Vol 6 (12) ◽  
pp. 4077-4086 ◽  
Author(s):  
Theresa L B Edelman ◽  
Katherine A McCulloch ◽  
Angela Barr ◽  
Christian Frøkjær-Jensen ◽  
Erik M Jorgensen ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Null Allele ◽  
Postembryonic Development ◽  
Developmental Timing ◽  
Critical Element ◽  
Coding Region ◽  
Protein Coding ◽  
Temporal Regulation ◽  
Larval Stages ◽  
Hypomorphic Alleles

Abstract The Caenorhabditis elegans heterochronic gene pathway regulates the relative timing of events during postembryonic development. lin-42, the worm homolog of the circadian clock gene, period, is a critical element of this pathway. lin-42 function has been defined by a set of hypomorphic alleles that cause precocious phenotypes, in which later developmental events, such as the terminal differentiation of hypodermal cells, occur too early. A subset of alleles also reveals a significant role for lin-42 in molting; larval stages are lengthened and ecdysis often fails in these mutant animals. lin-42 is a complex locus, encoding overlapping and nonoverlapping isoforms. Although existing alleles that affect subsets of isoforms have illuminated important and distinct roles for this gene in developmental timing, molting, and the decision to enter the alternative dauer state, it is essential to have a null allele to understand all of the roles of lin-42 and its individual isoforms. To remedy this problem and discover the null phenotype, we engineered an allele that deletes the entire lin-42 protein-coding region. lin-42 null mutants are homozygously viable, but have more severe phenotypes than observed in previously characterized hypomorphic alleles. We also provide additional evidence for this conclusion by using the null allele as a base for reintroducing different isoforms, showing that each isoform can provide heterochronic and molting pathway activities. Transcript levels of the nonoverlapping isoforms appear to be under coordinate temporal regulation, despite being driven by independent promoters. The lin-42 null allele will continue to be an important tool for dissecting the functions of lin-42 in molting and developmental timing.

The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans

Nature ◽  
2000 ◽  
Vol 403 (6772) ◽  
pp. 901-906 ◽  
Author(s):  
Brenda J. Reinhart ◽  
Frank J. Slack ◽  
Michael Basson ◽  
Amy E. Pasquinelli ◽  
Jill C. Bettinger ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Developmental Timing

scholarly journals The Conserved miR-51 microRNA Family Is Redundantly Required for Embryonic Development and Pharynx Attachment in Caenorhabditis elegans

Genetics ◽  
2010 ◽  
Vol 185 (3) ◽  
pp. 897-905 ◽  
Author(s):  
W. Robert Shaw ◽  
Javier Armisen ◽  
Nicolas J. Lehrbach ◽  
Eric A. Miska
Keyword(s):  
Caenorhabditis Elegans ◽  
Embryonic Development ◽  
Microrna Family
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