scholarly journals Long-Range Directional Movement of an Interphase Chromosome Site

2006 ◽  
Vol 16 (8) ◽  
pp. 825-831 ◽  
Author(s):  
Chien-Hui Chuang ◽  
Anne E. Carpenter ◽  
Beata Fuchsova ◽  
Terezina Johnson ◽  
Primal de Lanerolle ◽  
...  
Keyword(s):  
Long Range ◽  
Interphase Chromosome ◽  
Directional Movement

scholarly journals Long-Range Interphase Chromosome Organization in Drosophila: A Study Using Color Barcoded Fluorescence In Situ Hybridization and Structural Clustering Analysis

2004 ◽  
Vol 15 (12) ◽  
pp. 5678-5692 ◽  
Author(s):  
Michael G. Lowenstein ◽  
Thomas D. Goddard ◽  
John W. Sedat
Keyword(s):  
In Situ Hybridization ◽  
Structural Analysis ◽  
Fluorescence In Situ Hybridization ◽  
Long Range ◽  
Developmental Stages ◽  
Structural Features ◽  
Interphase Chromosome ◽  
Homologous Chromosomes ◽  
Cell Type Specific

We have developed a color barcode labeling strategy for use with fluorescence in situ hybridization that enables the discrimination of multiple, identically labeled loci. Barcode labeling of chromosomes provides long-range path information and allows structural analysis at a scale and resolution beyond what was previously possible. Here, we demonstrate the use of a three-color, 13-probe barcode for the structural analysis of Drosophila chromosome 2L in blastoderm stage embryos. We observe the chromosome to be strongly polarized in the Rabl orientation and for some loci to assume defined positions relative to the nuclear envelope. Our analysis indicates packing ∼15- to 28-fold above the 30-nm fiber, which varies along the chromosome in a pattern conserved across embryos. Using a clustering implementation based on rigid body alignment, our analysis suggests that structures within each embryo represent a single population and are effectively modeled as oriented random coils confined within nuclear boundaries. We also found an increased similarity between homologous chromosomes that have begun to pair. Chromosomes in embryos at equivalent developmental stages were found to share structural features and nuclear localization, although size-related differences that correlate with the cell cycle also were observed. The methodology and tools we describe provide a direct means for identifying developmental and cell type-specific features of higher order chromosome and nuclear organization.

Nuclear Envelope Dynamics During Mitosis

1988 ◽  
Vol 46 ◽  
pp. 224-225
Author(s):  
Brian Burke
Keyword(s):  
Nuclear Envelope ◽  
Membrane Vesicles ◽  
Nuclear Pore ◽  
Nuclear Pore Complexes ◽  
Animal Cells ◽  
Interphase Chromosome ◽  
Lamin B ◽  
Chromosome Architecture ◽  
Complex Membrane ◽  
Pore Complexes

The nuclear envelope is a complex membrane structure that forms the boundary of the nuclear compartment in eukaryotes. It regulates the passage of macromolecules between the two compartments and may be important for organizing interphase chromosome architecture. In interphase animal cells it forms a remarkably stable structure consisting of a double membrane ouerlying a protein meshwork or lamina and penetrated by nuclear pore complexes. The latter form the channels for nucleocytoplasmic exchange of macromolecules, At the onset of mitosis, however, it rapidly disassembles, the membranes fragment to yield small vesicles and the lamina, which is composed of predominantly three polypeptides, lamins R, B and C (MW approx. 74, 68 and 65 kDa respectiuely), breaks down. Lamins B and C are dispersed as monomers throughout the mitotic cytoplasm, while lamin B remains associated with the nuclear membrane vesicles.

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