Epithelial cell adhesion molecule expression (CD326) in cancer: A short review

2012 ◽  
Vol 38 (1) ◽  
pp. 68-75 ◽  
Author(s):  
Carlo Patriarca ◽  
Roberto Maria Macchi ◽  
Anja K. Marschner ◽  
Hakan Mellstedt
Keyword(s):  
Cell Adhesion ◽  
Epithelial Cell ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Short Review ◽  
Adhesion Molecule Expression ◽  
Epithelial Cell Adhesion Molecule

Epithelial Cell Adhesion Molecule Expression in Canine Tumours

2016 ◽  
Vol 155 (4) ◽  
pp. 299-304 ◽  
Author(s):  
D.H. Thamm ◽  
D.F. Hayes ◽  
T. Meuten ◽  
T. Laver ◽  
D.G. Thomas
Keyword(s):  
Cell Adhesion ◽  
Epithelial Cell ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Adhesion Molecule Expression ◽  
Epithelial Cell Adhesion Molecule

Usefulness of epithelial cell adhesion molecule expression in the algorithmic approach to Lynch syndrome identification

2013 ◽  
Vol 44 (3) ◽  
pp. 412-416 ◽  
Author(s):  
Eva Musulen ◽  
Ignacio Blanco ◽  
Cristina Carrato ◽  
Maria Teresa Fernandez-Figueras ◽  
Marta Pineda ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Epithelial Cell ◽  
Lynch Syndrome ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Adhesion Molecule Expression ◽  
Epithelial Cell Adhesion Molecule ◽  
Algorithmic Approach

Epithelial cell adhesion molecule expression in pituitary adenomas: an immunohistochemical study

2010 ◽  
Vol 14 (6) ◽  
pp. 418-424 ◽  
Author(s):  
Alma Ortiz-Plata ◽  
Karina Moreno-Leyva ◽  
Mario López-Gómez ◽  
Sandra Santos-Salinas ◽  
Aurora Sánchez-García ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Epithelial Cell ◽  
Adhesion Molecule ◽  
Pituitary Adenomas ◽  
Cell Adhesion Molecule ◽  
Immunohistochemical Study ◽  
Adhesion Molecule Expression ◽  
Epithelial Cell Adhesion Molecule

scholarly journals Solubility assessment of single-chain antibody fragment against epithelial cell adhesion molecule extracellular domain in four Escherichia coli strains

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Fatemeh Sadat Javadian ◽  
Majid Basafa ◽  
Aidin Behravan ◽  
Atieh Hashemi
Keyword(s):  
Escherichia Coli ◽  
Cell Adhesion ◽  
Epithelial Cell ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Extracellular Domain ◽  
Epithelial Cell Adhesion Molecule ◽  
Single Chain ◽  
E Coli ◽  
The Impact

Abstract Background Overexpression of the EpCAM (epithelial cell adhesion molecule) in malignancies makes it an attractive target for passive immunotherapy in a wide range of carcinomas. In comparison with full-length antibodies, due to the small size, the scFvs (single-chain variable fragments) are more suitable for recombinant expression in E. coli (Escherichia coli). However, the proteins expressed in large amounts in E. coli tend to form inclusion bodies that need to be refolded which may result in poor recovery of bioactive proteins. Various engineered strains were shown to be able to alleviate the insolubility problem. Here, we studied the impact of four E. coli strains on the soluble level of anti-EpEX-scFv (anti-EpCAM extracellular domain-scFv) protein. Results Although results showed that the amount of soluble anti-EpEX-scFv obtained in BL21TM (DE3) (114.22 ± 3.47 mg/L) was significantly higher to those produced in the same condition in E. coli RosettaTM (DE3) (71.39 ± 0.31 mg/L), and OrigamiTM T7 (58.99 ± 0.44 mg/L) strains, it was not significantly different from that produced by E. coli SHuffleTM T7 (108.87 ± 2.71 mg/L). Furthermore, the highest volumetric productivity of protein reached 318.29 ± 26.38 mg/L in BL21TM (DE3). Conclusions Although BL21TM (DE3) can be a suitable strain for high-level production of anti-EpEX-scFv protein, due to higher solubility yield (about 55%), E. coli SHuffleTM T7 seems to be better candidate for soluble production of scfv compared to BL21TM (DE3) (solubility yield of about 30%).

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