41. Cryopreservation of greenlipped mussel (Perna canaliculus) trochophore larvae

Cryobiology ◽  
2011 ◽  
Vol 63 (3) ◽  
pp. 317
Author(s):  
E. Paredes ◽  
S.L. Adams ◽  
S.L. Gale ◽  
L.T. McGowan ◽  
J.F. Smith ◽  
...  
Keyword(s):  
Perna Canaliculus

scholarly journals Furan fatty acid as an anti-inflammatory component from the green-lipped mussel Perna canaliculus

2011 ◽  
Vol 108 (42) ◽  
pp. 17533-17537 ◽  
Author(s):  
T. Wakimoto ◽  
H. Kondo ◽  
H. Nii ◽  
K. Kimura ◽  
Y. Egami ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Perna Canaliculus ◽  
Furan Fatty Acid ◽  
Anti Inflammatory ◽  
Inflammatory Component

An investigation of oxidative stress and antioxidant biomarkers during Greenshell mussel (Perna canaliculus) oocyte cryopreservation

2014 ◽  
Vol 82 (6) ◽  
pp. 779-789 ◽  
Author(s):  
Samantha L. Gale ◽  
David J. Burritt ◽  
H. Robin Tervit ◽  
Serean L. Adams ◽  
Lindsay T. McGowan
Keyword(s):  
Oxidative Stress ◽  
Oocyte Cryopreservation ◽  
Perna Canaliculus

scholarly journals Protein Identification Strategies for the Greenshell Mussel Perna canaliculus

2021 ◽  
Author(s):  
◽  
Cassidy Moeke
Keyword(s):  
Heavy Metal ◽  
Metal Pollution ◽  
Heavy Metal Pollution ◽  
Protein Identification ◽  
De Novo ◽  
De Novo Sequencing ◽  
Cytoskeletal Proteins ◽  
Est Database ◽  
Perna Canaliculus ◽  
Sequence Databases

<p>The greenshell mussel Perna canaliculus is considered to be a suitable biomonitor for heavy metal pollution. This is due to their ability to accumulate and tolerate heavy metals in their tissues. These characteristics make them useful for identifying protein biomarkers of heavy metal pollution, as well as proteins associated with heavy metal detoxification and homeostasis. However, the identification of such proteins is restricted by the greenshell mussel being poorly represented in sequence databases. Several strategies have previously been used to identify proteins in unsequenced species, but only one of these strategies has been applied to the greenshell mussel. The objective of this thesis was to examine different protein identification strategies using a combined two-dimensional gel electrophoresis and MALDI-TOF/TOF mass spectrometry approach. The protein identification strategies used include a Mascot database search, as well as de novo sequencing approaches using PEAKS DB and SPIDER homology searches. In total, 155 protein spots were excised and a total of 68 identified. Fifty-six proteins were identified using a Mascot search against the Mollusca, NCBInr and Invertebrate EST database, with seven single-peptide identifications. De novo sequencing strategies identified additional proteins, with two from a PEAKS DB search and 10 from an error-tolerant SPIDER homology search. The most noticeable protein groups identified were cytoskeletal proteins, stress response proteins and those involved in protein biosynthesis. Actin and tubulin made up the bulk of the identifications, accounting for 39% of all proteins identified. This multifaceted approach was shown to be useful for identifying proteins in the greenshell mussel Perna canaliculus. Mascot and PEAKS DB performed equally well, while the error-tolerant functionality of SPIDER was useful for identifying additional proteins. A subsequent search against the Invertebrate EST database was also found to be useful for identifying additional proteins. Despite this, more than half of all proteins remained unidentified. Most of these proteins either failed to produce good quality MS spectra or did not find a match to a sequence in the database. Future research should first focus on obtaining quality MS spectra for all proteins concerned and then examine other strategies that may be more suitable for identifying proteins for species with poor representation in sequence databases.</p>

Composition of beachcast material containing green-lipped mussel ( Perna canaliculus ) seed harvested for aquaculture in New Zealand

Aquaculture ◽  
2018 ◽  
Vol 488 ◽  
pp. 30-38 ◽  
Author(s):  
Andrew G. Jeffs ◽  
Natalí J. Delorme ◽  
Jenni Stanley ◽  
Leonardo N. Zamora ◽  
Carina Sim-Smith
Keyword(s):  
New Zealand ◽  
Perna Canaliculus
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