Biochemical characterization of acetylcholinesterase, cytochrome P450 and cross-resistance in an omethoate-resistant strain of Aphis gossypii Glover

2012 ◽  
Vol 31 (1) ◽  
pp. 15-20 ◽  
Author(s):  
Qingli Shang ◽  
Yiou Pan ◽  
Kui Fang ◽  
Jinghui Xi ◽  
James Andrew Brennan
Keyword(s):  
Cytochrome P450 ◽  
Resistant Strain ◽  
Biochemical Characterization ◽  
Aphis Gossypii ◽  
Cross Resistance ◽  
Aphis Gossypii Glover

Cross-resistance pattern and basis of resistance in a thiamethoxam-resistant strain of Aphis gossypii Glover

2017 ◽  
Vol 138 ◽  
pp. 91-96 ◽  
Author(s):  
Xiang Wei ◽  
Yiou Pan ◽  
Xuecheng Xin ◽  
Chao Zheng ◽  
Xiwu Gao ◽  
...  
Keyword(s):  
Resistant Strain ◽  
Aphis Gossypii ◽  
Cross Resistance ◽  
Resistance Pattern ◽  
Aphis Gossypii Glover

Characterization of P450 monooxygenase gene family in the cotton aphid, Aphis gossypii Glover

2022 ◽  
pp. 101861
Author(s):  
Likang Zhao ◽  
Chuanpeng Wang ◽  
Xueke Gao ◽  
Junyu Luo ◽  
Xiangzhen Zhu ◽  
...  
Keyword(s):  
Gene Family ◽  
Aphis Gossypii ◽  
Cotton Aphid ◽  
P450 Monooxygenase ◽  
Monooxygenase Gene ◽  
Aphis Gossypii Glover

scholarly journals Identification and characterization of cytochrome P450 1232A24 and 1232F1 from Arthrobacter sp. and their role in the metabolic pathway of papaverine

2019 ◽  
Vol 166 (1) ◽  
pp. 51-66 ◽  
Author(s):  
Jan M Klenk ◽  
Max-Philipp Fischer ◽  
Paulina Dubiel ◽  
Mahima Sharma ◽  
Benjamin Rowlinson ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Metabolic Pathway ◽  
Biochemical Characterization ◽  
Gene Clusters ◽  
Cytochrome P450 Monooxygenases ◽  
Dihydroxyphenylacetic Acid ◽  
Meta Position ◽  
Redox Partners

AbstractCytochrome P450 monooxygenases (P450s) play crucial roles in the cell metabolism and provide an unsurpassed diversity of catalysed reactions. Here, we report the identification and biochemical characterization of two P450s from Arthrobacter sp., a Gram-positive organism known to degrade the opium alkaloid papaverine. Combining phylogenetic and genomic analysis suggested physiological roles for P450s in metabolism and revealed potential gene clusters with redox partners facilitating the reconstitution of the P450 activities in vitro. CYP1232F1 catalyses the para demethylation of 3,4-dimethoxyphenylacetic acid to homovanillic acid while CYP1232A24 continues demethylation to 3,4-dihydroxyphenylacetic acid. Interestingly, the latter enzyme is also able to perform both demethylation steps with preference for the meta position. The crystal structure of CYP1232A24, which shares only 29% identity to previous published structures of P450s helped to rationalize the preferred demethylation specificity for the meta position and also the broader substrate specificity profile. In addition to the detailed characterization of the two P450s using their physiological redox partners, we report the construction of a highly active whole-cell Escherichia coli biocatalyst expressing CYP1232A24, which formed up to 1.77 g l−1 3,4-dihydroxyphenylacetic acid. Our results revealed the P450s’ role in the metabolic pathway of papaverine enabling further investigation and application of these biocatalysts.

Biochemical Characterization of the Human Liver Cytochrome P450 Arachidonic Acid Epoxygenase Pathway

1996 ◽  
Vol 330 (1) ◽  
pp. 87-96 ◽  
Author(s):  
Darryl C. Zeldin ◽  
Cindy R. Moomaw ◽  
Nate Jesse ◽  
Kenneth B. Tomer ◽  
Jeffrey Beetham ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Cytochrome P450 ◽  
Human Liver ◽  
Biochemical Characterization ◽  
Liver Cytochrome

Molecular and biochemical characterization of a sand fly population from Sri Lanka: evidence for insecticide resistance due to altered esterases and insensitive acetylcholinesterase

2005 ◽  
Vol 95 (4) ◽  
pp. 371-380 ◽  
Author(s):  
S.N. Surendran ◽  
S.H.P.P. Karunaratne ◽  
Z. Adamsn ◽  
J. Hemingway ◽  
N.J. Hawkes
Keyword(s):  
Sri Lanka ◽  
Insecticide Resistance ◽  
Biochemical Characterization ◽  
Preliminary Evidence ◽  
Resistance Mechanisms ◽  
Sand Fly ◽  
Cross Resistance ◽  
Cytochrome P450 Monooxygenases ◽  
Sand Flies

AbstractWith an increasing incidence of cutaneous leishmaniasis in Sri Lanka, particularly in northern provinces, insecticide-mediated vector control is under consideration. Optimizing such a strategy requires the characterization of sand fly populations in target areas with regard to species composition and extant resistance, among other parameters. Sand flies were collected by human bait and cattle-baited net traps on Delft Island, used as an illegal transit location by many refugees returning to the north of Sri Lanka from southern India where leishmaniasis is endemic. For species identification, genomic DNA was extracted and a fragment of the ribosomal 18S gene amplified. The sequence from all flies analysed matched that of Phlebotomus argentipes Annandale & Brunetti, the primary vector in India and the most likely vector in Sri Lanka. Independent morphological analysis also identified P. argentipes. To establish the current susceptibility status of vector species, data were obtained at the biochemical level, from which potential cross-resistance to alternative insecticides can be predicted. The Delft Island collection was assayed for the activities of four enzyme systems involved in insecticide resistance (acetylcholinesterase, non-specific carboxylesterases, glutathione-S-transferases and cytochrome p450 monooxygenases), establishing baselines against which subsequent collections can be evaluated. There was preliminary evidence for elevated esterases and altered acetylcholinesterase in this population, the first report of these resistance mechanisms in sand flies to our knowledge, which probably arose from the malathion-based spraying regimes of the Anti-Malarial Campaign.

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