scholarly journals Synthetic RNA switches as a tool for temporal and spatial control over gene expression

2012 ◽  
Vol 23 (5) ◽  
pp. 679-688 ◽  
Author(s):  
Andrew L Chang ◽  
Joshua J Wolf ◽  
Christina D Smolke
Keyword(s):  
Gene Expression ◽  
Spatial Control ◽  
Rna Switches ◽  
Temporal And Spatial

scholarly journals CreLite: An Optogenetically Controlled Cre/loxP System Using Red Light

2019 ◽  
Author(s):  
Shuo-Ting Yen ◽  
Kenneth A. Trimmer ◽  
Nader Aboul ◽  
Rachel D. Mullen ◽  
James C. Culver ◽  
...  
Keyword(s):  
Gene Expression ◽  
Light Exposure ◽  
Red Light ◽  
Regulatory Elements ◽  
Cell Labeling ◽  
P System ◽  
Zebrafish Embryos ◽  
Control Of Gene Expression ◽  
Spatial Control ◽  
Temporal And Spatial

ABSTRACTPrecise manipulation of gene expression with temporal and spatial control is essential for functional studies and the determination of cell lineage relationships in complex biological systems. The Cre-loxP system is commonly used for gene manipulation at desired times and places. However, specificity is dependent on the availability of tissue- or cell-specific regulatory elements used in combination with Cre or CreER (tamoxifen-inducible). Here we present CreLite, an optogenetically-controlled Cre system using red light in developing zebrafish embryos. Cre activity is disabled by splitting Cre and fusing the inactive halves with the Arabidopsis thaliana red light-inducible binding partners, PhyB and PIF6. In addition, PhyB-PIF6 binding requires phycocyanobilin (PCB), providing an additional layer of control. Upon exposure to red light (660 nm) illumination, the PhyB-CreC and PIF6-CreN fusion proteins come together in the presence of PCB to restore Cre activity. Red-light exposure of transgenic zebrafish embryos harboring a Cre-dependent multi-color fluorescent protein reporter (ubi:zebrabow) injected with CreLite mRNAs and PCB, resulted in Cre activity as measured by the generation of multi-spectral cell labeling in various tissues, including heart, skeletal muscle and epithelium. We show that CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development. CreLite provides a novel optogenetic tool for precise temporal and spatial control of gene expression in zebrafish embryos that may also be useful in cell culture, ex vivo organ culture, and other animal models for developmental biology studies.

scholarly journals Temporal and spatial control of gene expression in horticultural crops

2014 ◽  
Vol 1 (1) ◽  
Author(s):  
Manjul Dutt ◽  
Sadanand A Dhekney ◽  
Leonardo Soriano ◽  
Raju Kandel ◽  
Jude W Grosser
Keyword(s):  
Gene Expression ◽  
Control Of Gene Expression ◽  
Spatial Control ◽  
Horticultural Crops ◽  
Temporal And Spatial
Sign in / Sign up

Export Citation Format

Share Document