scholarly journals Current strategies for osteochondral regeneration: from stem cells to pre-clinical approaches

2011 ◽  
Vol 22 (5) ◽  
pp. 726-733 ◽  
Author(s):  
Márcia T Rodrigues ◽  
Manuela E Gomes ◽  
Rui L Reis
Keyword(s):  
Stem Cells ◽  
Osteochondral Regeneration

scholarly journals Attachment, Proliferation, and Chondroinduction of Mesenchymal Stem Cells on Porous Chitosan-Calcium Phosphate Scaffolds

2013 ◽  
Vol 7 (1) ◽  
pp. 275-281 ◽  
Author(s):  
Steven Elder ◽  
Anuhya Gottipati ◽  
Hilary Zelenka ◽  
Joel Bumgardner
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Calcium Phosphate ◽  
Treatment Options ◽  
Preliminary Investigation ◽  
Osteochondral Lesions ◽  
Porous Chitosan ◽  
Tissue Engineering Approach ◽  
Osteochondral Regeneration ◽  
Further Development

Symptomatic osteochondral lesions occur frequently, but relatively few treatment options are currently available. The purpose of this study was to conduct a preliminary investigation into a new tissue engineering approach to osteochondral regeneration. The concept is a biphasic construct consisting of a porous, osteoconductive chitosan-calcium phosphate scaffold supporting a layer of neocartilage formed by marrow-derived mesenchymal stem cells. Two experiments were conducted to assess the feasibility of this approach. The first experiment characterized the attachment efficiency and proliferation of primary human marrow-derived mesenchymal stem cells seeded relatively sparely onto the scaffold’s surface. The second experiment compared two different methods of creating a biphasic construct using a much higher density of primary porcine marrow stromal cells. About 40% of the sparsely seeded human cells attached and proliferated rapidly. Constructs formed by one of the two experimental techniques exhibited a layer of cartilaginous tissue which only partially covered the scaffold’s surface due to inadequate adhesion between the cells and the scaffold. This study demonstrates some potential for the approach to yield an implantable biphasic construct, but further development is required to improve cell-scaffold adhesion.

Tissue Engineering Strategies to Increase Osteochondral Regeneration of Stem Cells; a Close Look at Different Modalities

2021 ◽  
Author(s):  
Sepideh Saghati ◽  
Hamid Tayefi Nasrabadi ◽  
Ali Baradar Khoshfetrat ◽  
Keyvan Moharamzadeh ◽  
Ayla Hassani ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Osteochondral Regeneration

Induced pluripotent stem cells derived from different tissue sources and their prospect for osteochondral regeneration

2018 ◽  
Vol 32 (S1) ◽  
Author(s):  
Lubos Danisovic ◽  
Maria Csobonyeiova ◽  
Zuzana Varchulova Novakova ◽  
Andreas Nicodemou ◽  
Ivan Varga
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent ◽  
Osteochondral Regeneration

scholarly journals Osteochondral Regeneration Using Adipose Tissue-Derived Mesenchymal Stem Cells

2020 ◽  
Vol 21 (10) ◽  
pp. 3589 ◽  
Author(s):  
Daiki Murata ◽  
Ryota Fujimoto ◽  
Koichi Nakayama
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Articular Cartilage ◽  
Subchondral Bone ◽  
Three Dimensional ◽  
Cartilage Regeneration ◽  
Joint Disease ◽  
And Function ◽  
Osteochondral Regeneration

Osteoarthritis (OA) is a major joint disease that promotes locomotor deficiency during the middle- to old-age, with the associated disability potentially decreasing quality of life. Recently, surgical strategies to reconstruct both articular cartilage and subchondral bone for OA have been diligently investigated for restoring joint structure and function. Adipose tissue-derived mesenchymal stem cells (AT-MSCs), which maintain pluripotency and self-proliferation ability, have recently received attention as a useful tool to regenerate osteocartilage for OA. In this review, several studies were described related to AT-MSC spheroids, with scaffold and scaffold-free three-dimensional (3D) constructs produced using “mold” or “Kenzan” methods for osteochondral regeneration. First, several examples of articular cartilage regeneration using AT-MSCs were introduced. Second, studies of osteochondral regeneration (not only cartilage but also subchondral bone) using AT-MSCs were described. Third, examples were presented wherein spheroids were produced using AT-MSCs for cartilage regeneration. Fourth, osteochondral regeneration following autologous implantation of AT-MSC scaffold-free 3D constructs, fabricated using the “mold” or “Kenzan” method, was considered. Finally, prospects of osteochondral regeneration by scaffold-free 3D constructs using AT-MSC spheroids were discussed.

scholarly journals Characterization of Osteogenesis and Chondrogenesis of Human Decellularized Allogeneic Bone with Mesenchymal Stem Cells Derived from Bone Marrow, Adipose Tissue, and Wharton’s Jelly

2021 ◽  
Vol 22 (16) ◽  
pp. 8987
Author(s):  
Cheng-Fong Chen ◽  
Yi-Chun Chen ◽  
Yu-Show Fu ◽  
Shang-Wen Tsai ◽  
Po-Kuei Wu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Wharton’S Jelly ◽  
Allogeneic Bone ◽  
Wharton's Jelly ◽  
Bone Marrow Adipose Tissue ◽  
Marrow Adipose Tissue ◽  
Osteochondral Regeneration

Allogeneic bone grafts are a promising material for bone implantation due to reduced operative trauma, reduced blood loss, and no donor-site morbidity. Although human decellularized allogeneic bone (hDCB) can be used to fill bone defects, the research of revitalizing hDCB blocks with human mesenchymal stem cells (hMSCs) for osteochondral regeneration is missing. The hMSCs derived from bone marrow, adipose tissue, and Wharton’s jelly (BMMSCs, ADMSCs, and UMSCs, respectively) are potential candidates for bone regeneration. This study characterized the potential of hDCB as a scaffold for osteogenesis and chondrogenesis of BMMSCs, ADMSCs, and UMSCs. The pore sizes and mechanical strength of hDCB were characterized. Cell survival and adhesion of hMSCs were investigated using MTT assay and F-actin staining. Alizarin Red S and Safranin O staining were conducted to demonstrate calcium deposition and proteoglycan production of hMSCs after osteogenic and chondrogenic differentiation, respectively. A RT-qPCR was performed to analyze the expression levels of osteogenic and chondrogenic markers in hMSCs. Results indicated that BMMSCs and ADMSCs exhibited higher osteogenic potential than UMSCs. Furthermore, ADMSCs and UMSCs had higher chondrogenic potential than BMMSCs. This study demonstrated that chondrogenic ADMSCs- or UMSCs-seeded hDCB might be potential osteochondral constructs for osteochondral regeneration.

scholarly journals Osteochondral regeneration using constructs of mesenchymal stem cells made by bio three‐dimensional printing in mini‐pigs

2019 ◽  
Vol 37 (6) ◽  
pp. 1398-1408 ◽  
Author(s):  
Atsushi Yamasaki ◽  
Yoshihiro Kunitomi ◽  
Daiki Murata ◽  
Takafumi Sunaga ◽  
Tomohide Kuramoto ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Three Dimensional ◽  
Three Dimensional Printing ◽  
Osteochondral Regeneration ◽  
Dimensional Printing ◽  
Mini Pigs

scholarly journals Exosomes derived from human embryonic mesenchymal stem cells promote osteochondral regeneration

2016 ◽  
Vol 24 (12) ◽  
pp. 2135-2140 ◽  
Author(s):  
S. Zhang ◽  
W.C. Chu ◽  
R.C. Lai ◽  
S.K. Lim ◽  
J.H.P. Hui ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteochondral Regeneration
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