Laser-induced fluorescence imaging method to monitor citrus greening disease

2011 ◽  
Vol 79 (1) ◽  
pp. 90-93 ◽  
Author(s):  
Fabíola Manhas Verbi Pereira ◽  
Débora Marcondes Bastos Pereira Milori ◽  
Edenir Rodrigues Pereira-Filho ◽  
André Leonardo Venâncio ◽  
Mariana de Sá Tavares Russo ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Laser Induced Fluorescence ◽  
Citrus Greening ◽  
Imaging Method ◽  
Citrus Greening Disease ◽  
Greening Disease

Identification of citrus greening disease using a visible band image analysis

2013 ◽  
Author(s):  
Alireza Pourreza ◽  
Won Suk (Daniel) Lee ◽  
Eran Raveh ◽  
Youngki Hong ◽  
Hyuck-Joo Kim
Keyword(s):  
Image Analysis ◽  
Citrus Greening ◽  
Citrus Greening Disease ◽  
Visible Band ◽  
Greening Disease ◽  
Band Image

Molecular and cellular analysis of orange plants infected with Huanglongbing (citrus greening disease)

2020 ◽  
Vol 92 (2) ◽  
pp. 333-343
Author(s):  
Xing-Hai Zhang ◽  
Nicholas Pizzo ◽  
Mohamed Abutineh ◽  
Xiao-Lu Jin ◽  
Sarah Naylon ◽  
...  
Keyword(s):  
Citrus Greening ◽  
Citrus Greening Disease ◽  
Cellular Analysis ◽  
Greening Disease

scholarly journals Asian citrus psyllid adults inoculate huanglongbing bacterium more efficiently than nymphs when this bacterium is acquired by early instar nymphs

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
El-Desouky Ammar ◽  
Justin George ◽  
Kasie Sturgeon ◽  
Lukasz L. Stelinski ◽  
Robert G. Shatters
Keyword(s):  
Latent Period ◽  
Salivary Glands ◽  
Emerging Adults ◽  
Citrus Greening ◽  
Asian Citrus Psyllid ◽  
Diaphorina Citri ◽  
Citrus Greening Disease ◽  
Early Instar ◽  
Liberibacter Asiaticus ◽  
Greening Disease

Abstract The Asian citrus psyllid (Diaphorina citri) transmits the bacterium ‘Candidatus Liberibacter asiaticus’ (CLas), which causes huanglongbing (citrus greening) disease, in a circulative-propagative manner. We compared CLas inoculation efficiency of D. citri nymphs and adults into healthy (uninfected) citron leaves when both vector stages were reared from eggs on infected plants. The proportion of CLas-positive leaves was 2.5% for nymphs and 36.3% for adults. CLas acquisition by early instar nymphs followed by dissections of adults and 4th instar nymphs revealed that CLas bacterium had moved into the head-thorax section (containing the salivary glands) in 26.7–30.0% of nymphs and 37–45% of adults. Mean Ct values in these sections were 31.6–32.9 and 26.8–27.0 for nymphs and adults, respectively. Therefore, CLas incidence and titer were higher in the head-thorax of adults than in nymphs. Our results suggest that following acquisition of CLas by early instar D. citri nymphs, emerging adults inoculate the bacteria into citrus more efficiently than nymphs because adults are afforded a longer latent period necessary for multiplication and/or translocation of CLas into the salivary glands of the vector. We propose that CLas uses D. citri nymphs mainly for pathogen acquisition and multiplication, and their adults mainly for pathogen inoculation and spread.

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