Magnetic particles for in vitro molecular diagnosis: From sample preparation to integration into microsystems

2017 ◽  
Vol 158 ◽  
pp. 1-8 ◽  
Author(s):  
Tienrat Tangchaikeeree ◽  
Duangporn Polpanich ◽  
Abdelhamid Elaissari ◽  
Kulachart Jangpatarapongsa
Keyword(s):  
Sample Preparation ◽  
Molecular Diagnosis ◽  
Magnetic Particles

scholarly journals Magnetic Particles for Advanced Molecular Diagnosis

Materials ◽  
2019 ◽  
Vol 12 (13) ◽  
pp. 2158 ◽  
Author(s):  
Cristina Chircov ◽  
Alexandru Mihai Grumezescu ◽  
Alina Maria Holban
Keyword(s):  
Gene Expression ◽  
Infectious Disease ◽  
Nucleic Acid ◽  
Sample Preparation ◽  
Molecular Diagnosis ◽  
Magnetic Particles ◽  
Early Stage ◽  
Disease Diagnosis ◽  
Laboratory Medicine ◽  
Clinical Reality

Molecular diagnosis is the field that aims to develop nucleic-acid-based analytical methods for biological markers and gene expression assessments by combining laboratory medicine and molecular genetics. As it gradually becomes a clinical reality, molecular diagnosis could benefit from improvements resulting from thorough studies that could enhance the accuracy of these methods. The application of magnetic particles in molecular diagnosis tools has led to tremendous breakthroughs in terms of specificity, sensitivity, and discrimination in bioassays. Therefore, the aim of this review is to highlight the principles involved in the implementation of magnetic particles for sample preparation and targeted analyte isolation, purification, and extraction. Furthermore, the most recent advancements in the area of cancer and infectious disease diagnosis are presented, with an emphasis on screening and early stage detection.

scholarly journals Automated Sample Preparation and Data Collection Workflow for High-Throughput In Vitro Metabolomics

Metabolites ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 52
Author(s):  
Julia M. Malinowska ◽  
Taina Palosaari ◽  
Jukka Sund ◽  
Donatella Carpi ◽  
Gavin R. Lloyd ◽  
...  
Keyword(s):  
Sample Preparation ◽  
High Throughput ◽  
High Throughput Screening ◽  
In Vitro Screening ◽  
Chemical Safety ◽  
Experimental Conditions ◽  
Well Location ◽  
Relative Standard ◽  
Automated Sample Preparation

Regulatory bodies have started to recognise the value of in vitro screening and metabolomics as two types of new approach methodologies (NAMs) for chemical risk assessments, yet few high-throughput in vitro toxicometabolomics studies have been reported. A significant challenge is to implement automated sample preparation of the low biomass samples typically used for in vitro screening. Building on previous work, we have developed, characterised and demonstrated an automated sample preparation and analysis workflow for in vitro metabolomics of HepaRG cells in 96-well microplates using a Biomek i7 Hybrid Workstation (Beckman Coulter) and Orbitrap Elite (Thermo Scientific) high-resolution nanoelectrospray direct infusion mass spectrometry (nESI-DIMS), across polar metabolites and lipids. The experimental conditions evaluated included the day of metabolite extraction, order of extraction of samples in 96-well microplates, position of the 96-well microplate on the instrument’s deck and well location within a microplate. By using the median relative standard deviation (mRSD (%)) of spectral features, we have demonstrated good repeatability of the workflow (final mRSD < 30%) with a low percentage of features outside the threshold applied for statistical analysis. To improve the quality of the automated workflow further, small method modifications were made and then applied to a large cohort study (4860 sample infusions across three nESI-DIMS assays), which confirmed very high repeatability of the whole workflow from cell culturing to metabolite measurements, whilst providing a significant improvement in sample throughput. It is envisioned that the automated in vitro metabolomics workflow will help to advance the application of metabolomics (as a part of NAMs) in chemical safety, primarily as an approach for high throughput screening and prioritisation.

Sample Preparation for Lab-on-a-Chip Systems in Molecular Diagnosis: A Review

2021 ◽  
Author(s):  
Mylena Lemes Cunha ◽  
Stella Schuster da Silva ◽  
Mateus Cassaboni Stracke ◽  
Dalila Luciola Zanette ◽  
Mateus Nóbrega Aoki ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Molecular Diagnosis ◽  
Lab On A Chip

A sensitive HPLC-FL method to simultaneously determine febuxostat and diclofenac in rat plasma: assessment of metabolic drug interactions in vitro and in vivo

2020 ◽  
Vol 12 (16) ◽  
pp. 2166-2175 ◽  
Author(s):  
Dong-Gyun Han ◽  
Kyu-Sang Kim ◽  
Seong-Wook Seo ◽  
Young Mee Baek ◽  
Yunjin Jung ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Drug Interactions ◽  
Simultaneous Determination ◽  
Small Volume ◽  
Rat Plasma ◽  
Metabolic Drug ◽  
Simple Sample Preparation

We developed a sensitive, simple and validated HPLC-FL method for simultaneous determination of FEB and DIC in rat plasma. The method requires a relatively small volume of sample, has simple sample preparation and excellent sensitivity.

Comparative Investigations of Structure and Absorptive Capacity of Nano- and Microsized Magnetic-Operated Particles for their Application in Medicine and Biology

2009 ◽  
Vol 152-153 ◽  
pp. 403-406 ◽  
Author(s):  
Yu.G. Yanovsky ◽  
L.Kh. Komissarova ◽  
A.N. Danilin ◽  
E.I. Zaraysky
Keyword(s):  
Hepatitis B ◽  
Absorptive Capacity ◽  
Magnetic Particles ◽  
Cobalt Ferrite ◽  
Virus Hepatitis ◽  
Sorption Efficiency ◽  
Magnetite Fe3o4 ◽  
First Time ◽  
Different Molecular Weight

For the first time, the comparative investigations of structure and sorption efficiency of nano- and microsized magnetic-operated particles in respect to both the antigen and virus hepatitis B and also to the substance-markers (low-, middle- and highmolecular substances) were carried out. The sorption efficiency of magnetic particles: magnetite (Fe3O4), cobalt-ferrite (CoFe2O4), composite ferro-carbon (FeC) to the substances of different molecular weight was evaluated in vitro experiments. The high absorptive capacity to low- and highmolecular substance-markers has been observed for microsized composites of FeC. Absorptive capacity of the nanosized Fe3O4 and CoFe2O4 particles to the substance-markers was low. But the nanosized Fe3O4 and CoFe2O4 particles had a good absorptive capacity to virus of hepatitis B and its antigen (HBsAg). The sorption efficiency results of nano- and microsized magnetic particles correlate with the structure of their surface.

scholarly journals IMPROVEMENT OF POTATO SEED MATERIAL AND ITS DIAGNOSTICS IN SYSTEM OF BIOTECHNOLOGY

2012 ◽  
Vol 14 ◽  
pp. 156-166
Author(s):  
T.M. Oleynik ◽  
K.A. Sloblodyan ◽  
S.A. Slobodyan ◽  
R.V. Gricay
Keyword(s):  
Molecular Diagnosis ◽  
Potato Virus ◽  
Antiviral Drugs ◽  
Field Conditions ◽  
Potato Seed ◽  
Potato Varieties ◽  
In Vitro Plants ◽  
Seed Material ◽  
Potato Virus M

The results of improvement studies of potato varieties by chemotherapy along with the use of antiviral drugs: RNA-ase, acyclovir, izatizon, and hydrochloride as well as data on the molecular diagnosis of X-and M-viruses in vitro plants, resulting from the recovery are presented. 3 lines free from virus X and 4 lines free from potato virus M were allocated. After the testing of variety changeability and its economically valuable characteristics in field conditions one of them will be selected and submitted to the Bank in vitro redeveloped varieties.

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